The Genetic Diversity of Some Citrus Varieties from North of Iran Using Microsatellite Molecular Markers

Subject Areas : Journal of Plant Ecophysiology

Babak Babakhani ¹ ( Department of Biology, Islamic Azad University, Tonkabon Branch, Tonkabon, Iran )
Yalda Naghashi ² ( Department of Biology, Islamic Azad University, Tonkabon Branch, Tonkabon, Iran )
Seyed Afshin Hosseini Boldaji ³ ( Department of Biology, Yadegare- Imam Khomeini (RAH) Branch, Islamic Azad University, Tehran, Iran )
Mahdieh Hoshani ⁴ ( Department of Biology, Islamic Azad University, Tonkabon Branch, Tonkabon, Iran )

Received: 2016-10-29 Accepted : 2019-04-30 Published : 2020-06-21

Keywords: Cluster analysis, genotype, citrus, Category, microsatellite marker,

Abstract :

AbstractThe genetic diversity of 29 varieties of citrus, including varieties of orange, mandarin, bergamot, pomelo, and natural types through using eight microsatellite primers were evaluated. Genomic DNA extracted from the samples using the amplification was reproduced and products using polyacrylamide gel were electrophoresed. A total of 97 bands were scored for marker ISSR, 78 bands were polymorphic bands of 80.22 percent. The highest and the lowest percentage of polymorphic were ISSR-8 primers with 90% and polymorphic ISSR-5 with 73% band, respectively. The average value of PIC was 0.18 in this test. The most of the PIC was reported by primer ISSR-6 and ISSR-8 with 0.27 and the lowest primer was also reported by ISSR-1 with 0.12. Based on the study, dendrogram of cluster resulted from the analysis of UPGMA method with simple matching coefficient; the cultivars were classified into five main groups. In this study, Pomelo was in a separate cluster. The ISSR molecular marker of Satsuma mandarin and clementine Suji Yama were grouped into two distinct groups. Therefore, this molecular marker presents the different levels of diversity at the genome level which can be useful in germplasm management and hereditary stocks. Genetic diversity analysis and kinship relationships in citrus provide useful information for breeding programs, selection, and registration of new varieties.

References:

شفیعی زرگر، ع.، ر. اسلامی زاده. 1384. ارزیابی و شناسایی درختان مرکبات دارای تنوع ژنتیکی بارز در منطقه شمال خوزستان. چهارمین کنگره علوم باغبانی ایران. 17-19 آبان ماه، دانشگاه فردوسی مشهد،76 صفحه.

گلعین ب. 1384. شناسایی و جداسازی ریزماهواره های مرکبات به منظور تشخیص هیبریدها و ارزیابی پرتقال و نارنگی ایرانی. پایان نامه دکترا. دانشکده کشاورزی دانشگاه تهران. 117

نقوی، م. ر.، ب. قره یاضی .، ق. حسینی سالکده. 1388. نشانگرهای مولکولی. انتشارات دانشگاه تهران. 330-334 .

Barkley, N.A., M.L. Roose,   R.R. Krueger and C.T. Federici. 2006. Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs). Theor. Appl. Genet. 112: 1519–1531.

Bernet, G.P., P.F. Mestre, J.A. Pina and M.J. Asins. 2004. Molecular discrimination of lemon cultivars. HortSci. 39: 165-169.

Campos, E.T., M.A.G. Espinosa, M.L. Warburton, A.S. Varela and A.V. Monter. 2005. Characterization of Mandarin (Citrus spp.) using morphological and AFLP markers. Interciencia. 30 (11):687-693.

Dehestani, A., S.k. Kazemitabar and H. Rahimian.  2007. Assessment of Genetic Diversity of Navel Sweet Orange Cultivars Grown in Mazandaran Province Using RAPD Markers. Asi. J. of Plant Sci. 6(7):1119-1124.

Fang, D.Q. and M.L. Roose .1997. Identification of closely related Citrus cultivars with intersimple sequence repeat markers. Theor. Appl. Genet. 95: 408–417.

Ghanbari, A., .N.B. Jelodar and H. Rahimiyan. 2009. Studying genetic diversity in Satsuma (Citrus unshiu) mandarin utilizing microsatellite markers. Int. J. of Agr. Res. 4(2):88-96.

Golein, B., M. Bigonah, M. Azadvar and M. Golmohammadi. 2012. Analysis of genetic relationship between ‘Bakraee’ (Citrus sp.) and some known Citrus genotypes through SSR and PCR-RFLP markers. Sci. Hort. 148:147–153.

Jannati, M., R. Pourjanabad and Z. Salehi. 2009. Genetic diversity analysis of Iranian citrus varieties using micro satellite (SSR) based markers. J of Hort and Forest. 1(7): 120-125.

Malik, S.K., M.R. Rohini, S. Kumar, R. Choudhary, D.  Pal, and R. Chaudhury. 2012. Assessment of genetic diversity in sweet orange [Citrus sinensis (L.) Osbeck] cultivars of India using morphological and RAPD markers. Agri. Res. 1(4):317–324.

Mohammadi, S.A. and B.M.  Prasanna. 2003. Analysis of genetic diversity in crop plants: Salient statistical tools and considerations. Crop Sci. 43: 1235-1248.

Murry, M.G.,  and W.F. Thompson.  1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: 4321-4325.

Rouhi Ghorabaie, H.R., F.R. Ghazvini, B. Golein and A.R. Nabipour. 2010. Identification of some Citrus accessions in a Citrus germplasm utilizing simple sequence repeat markers (SSRs). Hort. Envir. Biotchnol. 51: 343–347.

Shahsavar, A.R., K. Izadpanah, E. Tafazoli and B.E.S. Tabatabaei .2007. Characterization of citrus germplasm including unknown variants by inter-simple sequence repeat (ISSR) markers. Sci. Hort. 112: 310-314.

Soost, R.K. and M.L. Roose .1996. Citrus In: Fruit Breeding. Tree and Tropical Fruits. John Wiley and Sons, pp. 257–323.

Tripolitsiotis, C., N. Nikoloudakis, A. Linos and M. Hagidimitriou. 2013. Molecular Characterization and Analysis of the Greek Citrus Germplasm. Not. Bot. Hort. Agri. 41(2):463-471.

Uzun, A., O. Gulsen, U.  Seday, T. Yesiloglu, Y. Aka-kacar and O. Tuzcu. 2011. Investigation of genetic relationshipsamong trifoliata oranges and their hybrid relatives based on ISSR markers. Rom. Biotechnol. Lett. 4:6430-6438.

Uzun, A., O. Gulsen, T.  Yesiloglu, Y.  Aka-Kacar and O. Tuzcu .2012. Distinguishing grapefruit and pummelo accessions using ISSR markers. Czech J. Genet. Plant Breed. 46: 170–177.