انتقال ژنتیکی ژن آمیلاز به سویه باکتروئید شکمبه توسط کانژوگاسیون نتیجه آن بهبود آنزیم شکمبه
Subject Areas : Camel
ح. عقبی طلب
1
(Department of Animal Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
ف. مرادیان
2
(Department of Basic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
ق. رحیمی
3
(Department of Animal Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
ح. رحیمیان
4
(Department of Plant Protection, Sari Agricultural Science and Natural Resources University, Sari, Iran)
Keywords: ژن 16S rRNA, سویه باکتروئید, کانژوگاسیون, دستورزی, وکتور انتقالی,
Abstract :
سویههای باکتریهای شکمبهای قابلیت دستکاری برای انجام عملکرد متفاوت از نوع وحشی را دارند. بیشترین تعداد سویههای باکتری شکمبه و روده از خانواده باکتروئیدها هستند که توانایی تغییر ژنتیکی جهت تولید آنزیم را دارند. یکی از دستورزی ژنتیکی باکتری شکمبه که میتواند انجام شود، تولید آنزیم هضم کننده نشاسته جهت افزایش جریان مواد غذایی به شکمبه است. در مطالعه حاضر، سویههای باکتروئید از شکمبه گاو جدا شدند. ازآنالیز ژن 16S rRNA جهت تأیید طبقهبندی گونه باکتروئیدی استفاده شد. ژن آمیلاز در وکتور بیانی pET28A همسانهسازی شد و سپس در وکتور انتقالی pGFK114.1 باکتروئید-اشرشیاکولی زیر همسانهسازی گردید. کانژوگاسیون بین اشرشیاکولی و سویه باکتروئیدی با دو دهنده اشرشیاکولی شامل وکتورهایpGFK114.1 و pRK231 و یک سویه دریافت کننده باکتروئیدی انجام شد. انتقال ژن آمیلاز توسط مارکر ژن مقاومت به آنتی بیوتیک سفوکسیتین در وکتور تأییدگردید. سنجش فعالیت آمیلاز نشان داد که بیان آنزیم در سویه باکتروئید شکمبهای به طور موفقیت آمیزی انجام گرفته است.
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