ارزیابی نانو و میکروکپسول‌های سیلی‌مارین در شرایط شبیه‌سازی شده دستگاه گوارش برای رسانش هدفمند در حیوان

Subject Areas : Camel

س. یوسف‌دوست ¹ (Department of Animal and Poultry Physiology, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)
ف. صمدی ² (Department of Animal and Poultry Physiology, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)
س.م. جعفری ³ (Department of Food Materials and Process Design Engineering, Faculty of Food Science and Technology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)
س.س. رمضان‌پور ⁴ (Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)
ف. گنجی ⁵ (Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran)
س. حسنی ⁶ (Department of Animal and Poultry Physiology, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)

Received: 2018-05-20 Accepted : 2019-08-29 Published : 2019-06-01

Keywords: فراصوت, خارمریم, نانو و میکروکپسول, شرایط شبیه‌سازی شده دستگاه گوارش,

Abstract :

هدف اصلی این مطالعه، مقایسه سرعت رهایش نانو و میکروکپسول­های حاوی عصاره خارمریم در شرایط شبیه­سازی شده معدی و روده­ای حیوان بود. عصاره با روش امولسیفیکاسیون/ ژله­ای شدن داخل آلژینات سدیم کپسوله گردید. اندازه ذره، توان زتا، شاخص پراکندگی، و مورفولوژی نانوکپسول­ها به کمک تفرق نور پویا و میکروسکوپ الکترونی عبوری بررسی شدند. به علاوه، اثر فراصوت بر خواص نانوکپسول و پروفایل رهایش نانو و میکروکپسول­های حاوی عصاره خار مریم مورد ارزیابی قرار گرفت. نتایج نشان داد که فراصوت با کاهش اندازه کپسول­ها از 5/657 نانومتر به 1/169 نانومتر منجر به ایجاد ذرات یکنواخت با شاخص پراکندگی پایین گردید. راندمان کپسولاسیون نانوکپسول­ها 61 درصد محاسبه شد. نانو و میکروکپسول­های آلژینات با محافظت از پلی­فنول­ها در شرایط اسیدی معدی به ترتیب منجر به راهایش 10 و 12 درصد شدند. نانوکپسول­ها، در محیط شبیه­سازی شده روده محتویات خود را سریعتر و به میزان بیشتری آزاد کردند. در مجموع، نانوکپسول­های آلژینات حاوی عصاره خارمریم با راهایش بیش از 90 درصد در محیط شبیه­سازی شده روده، با موفقیت تهیه شدند که از این مورد می­توان در اهداف رسانش هدفمند برای مدل­های حیوانی استفاده کرد.

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