ارزیابی نانو و میکروکپسولهای سیلی‌مارین در شرایط شبیهسازی شده دستگاه گوارش برای رسانش هدفمند در حیوان
Subject Areas : Camelس. یوسف‌دوست 1 , ف. صمدی 2 , س.م. جعفری 3 , س.س. رمضان‌پور 4 , ف. گنجی 5 , س. حسنی 6
1 - Department of Animal and Poultry Physiology, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
2 - Department of Animal and Poultry Physiology, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
3 - Department of Food Materials and Process Design Engineering, Faculty of Food Science and Technology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
4 - Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
5 - Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
6 - Department of Animal and Poultry Physiology, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
Keywords: فراصوت, خارمریم, نانو و میکروکپسول, شرایط شبیهسازی شده دستگاه گوارش,
Abstract :
هدف اصلی این مطالعه، مقایسه سرعت رهایش نانو و میکروکپسولهای حاوی عصاره خارمریم در شرایط شبیهسازی شده معدی و رودهای حیوان بود. عصاره با روش امولسیفیکاسیون/ ژلهای شدن داخل آلژینات سدیم کپسوله گردید. اندازه ذره، توان زتا، شاخص پراکندگی، و مورفولوژی نانوکپسولها به کمک تفرق نور پویا و میکروسکوپ الکترونی عبوری بررسی شدند. به علاوه، اثر فراصوت بر خواص نانوکپسول و پروفایل رهایش نانو و میکروکپسولهای حاوی عصاره خار مریم مورد ارزیابی قرار گرفت. نتایج نشان داد که فراصوت با کاهش اندازه کپسولها از 5/657 نانومتر به 1/169 نانومتر منجر به ایجاد ذرات یکنواخت با شاخص پراکندگی پایین گردید. راندمان کپسولاسیون نانوکپسولها 61 درصد محاسبه شد. نانو و میکروکپسولهای آلژینات با محافظت از پلیفنولها در شرایط اسیدی معدی به ترتیب منجر به راهایش 10 و 12 درصد شدند. نانوکپسولها، در محیط شبیهسازی شده روده محتویات خود را سریعتر و به میزان بیشتری آزاد کردند. در مجموع، نانوکپسولهای آلژینات حاوی عصاره خارمریم با راهایش بیش از 90 درصد در محیط شبیهسازی شده روده، با موفقیت تهیه شدند که از این مورد میتوان در اهداف رسانش هدفمند برای مدلهای حیوانی استفاده کرد.
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