تاثیر محلول پاشی با کیتوزان بر عملکرد و اسانس گیاه مرزه (Satureja isophella L) تحت تنش شوری
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)سمیه صالحی 1 , زهرا رضایتمند 2
1 - Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2 - Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Keywords: آنتی اکسیدان, شوری, مرزه, کیتوزان,
Abstract :
مقدمه و هدف:گیاه مرزه Satureja isophella L.)) یکی از گیاهان دارویی متعلق به خانواده نعنا است. تنش شوری در رشد و نمو و کیفیت و کمیت اسانس گیاهان دارویی اثر دارد. نقش کیتوزان نیز به عنوان یک الیسیتور زیستی در بهبود تولید مواد ثانویه گیاهان دارویی عنوان شده است. تحقیق حاضر به منظور بررسی اثر غلظت های مختلف کیتوزان بر برخی شاخص های رشد و کمیت و کیفیت اسانس گیاه مرزه تحت تنش شوری انجام گرفت. روش تحقیق: در این تحقبق گیاه مرزه تحت تیمارهای کیتوزان در سه سطح (4/0، 2/0 و 0) درصد و تیمار با آب نمک (Nacl) در غلظت های (100، 50 و 0) میلی مولار قرار گرفت. پس از دو هفته از تیمار دهی وزن خشک ریشه و ساقه و ارتفاع گیاه و تعداد برگ و شاخه های جانبی گیاه اندازه گیری شد، همچنین تجزیه و شناسایی اجزاءاسانس گیاه بااستفاده از دستگاه GC/MS انجام گرفت. نتایج وبحث: نتایج این تحقیق نشان می دهد که تنش شوری باعث کاهش وزن خشک ریشه و ساقه و ارتفاع گیاه و تعداد برگ و شاخه های جانبی گیاه می شود و تیمار کیتوزان می تواند این کاهش را تعدیل نماید. تیمار متقابل کلرید سدیم در غلظت 50 میلی مولار وهمچنین کیتوزان در غلظت 2/0 در صد اثر معنی داری بر روی افزایش حجم و کیفیت اسانس در گیاه مرزه دارد. که با توجه به نتایج بدست آمده از آنالیز GC/MS 14 ترکیب اصلی در اسانس تشخیص داده شد که بیشترین ترکیبات P-Cymene به میزان 13/3 ، Y-Terpinene به میزان 97/28 و Caryacrolبه میزان 64/59 بدست آمده است. نتایج نشان می دهد که با افزایش تنش شوری در غلظت 100 میلی مولار نمک و غلظت 4/0 کیتوزان بیشترین میزان کارواکرول و در غلظت 50 میلی مولار نمک و 4/0 کیتوزان بیشترین میزان P-Cymene و Y-Terpinene بدست آمده است.
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