فعالیت آنتی اکسیدانی، ترکیبات پلی فنولی و همبستگی بین آنها در کاسبرگ گیاه چای ترش

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

حکیمه علومی ¹ , شهریار شاکری ² , مجید بهزادی ³

1 - Department of Ecology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
2 - Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
3 - Agricultural Research, Education and Extension Organization, Kerman Center, Kerman, Iran

Received: 2015-04-19 Accepted : 2016-01-20 Published : 2016-07-01

Keywords: فلاونوئیدها, آنتوسیانین, چای ترش, ترکیبات فنولی, آنزیم های آنتی اکسیدان,

Abstract :

مقدمه و هدف: عصاره کاسبرگ چای ترش غنی از ترکیبات آنتی اکسیدانی طبیعی می‌باشد. در این پژوهش بررسی همبستگی فعالیت آنتی اکسیدانی و ترکیبات فنولی گیاه چای ترش مورد بررسی قرار گرفت. روش تحقیق: محتوای ترکیبات فنولی کل، فلاونوئیدها و آنتوسیانین ها بصورت اسپکتروفتومتری بررسی شد. فعالیت آنزیم های کاتالاز و پروکسیداز در میلی گرم پروتئین محاسبه شد. فعالیت آنتی اکسیدانی عصاره بر اساس روش فسفومولیبدات، درصد مهارDPPH و قدرت احیا فریک مورد بررسی قرارگرفت. نتایج و بحث: کاسبرگ چای ترش غنی از ترکیبات فنولی، فلاونوئیدها و آنتوسیانین می‌باشد. فعالیت کاتالاز و پراکسیداز معادل 13/3 و 05/0 واحد در میلی گرم پروتئین بود. بر اساس روش DPPH فعالیت آنتی اکسیدانی عصاره کاسبرگ بسیار بالا می‌باشد. آزمون همبستگی بین پارامترهای مورد بررسی انجام شد. نتایج همبستگی وجود رابطه بین محتوای ترکیبات فنولی، فلاونوئیدها، آنتوسیانین‌ها و ظرفیت انتی اکسیدانی کاسبرگ گیاه چای ترش را در سطح احتمال 5%  تایید می‌کند. توصیه‌های کاربردی: عصاره کاسبرگ چای ترش غنی از ترکیبات آنتی اکسیدانی شامل ترکیبات فنولی، فلاونوئیدها و آنتوسیانین می‌باشد. براساس روش های سنجش ظرفت آنتی اکسیدانی، کاسبرگ‌ها فعالیت آنتی اکسیدانی قابل قبولی دارند. بنابراین، عصاره این گیاه بعنوان منبع طبیعی ترکیبات اکسیدان قابل استفاده در صنایع غذایی و داروی گیاهی معرفی می‌گردد.

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