Phytochemical analysis and screening of antioxidant, antibacterial and anti-inflammatory activity of essential oil of Premna mucronata Roxb. leaves

Subject Areas : Essential Oils: Classical and Advanced Techniques for Isolation and Biological Activities

Diksha Palariya ¹ , Anmol Singh ² , Anamika Dhami ³ , Anil K. Pant ⁴ , Ravendra Kumar ⁵ , Om Prakash ⁶

1 - Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, U.S. Nagar, 263145 Uttarakhand, India
2 - Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, U.S. Nagar, 263145 Uttarakhand, India
3 - Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, U.S. Nagar, 263145 Uttarakhand, India
4 - Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, U.S. Nagar, 263145 Uttarakhand, India
5 - Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, U.S. Nagar, 263145 Uttarakhand, India
6 - Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, U.S. Nagar, 263145 Uttarakhand, India

Received: 2019-07-20 Accepted : 2019-12-21 Published : 2019-12-01

Keywords: <i>Premna mucronata</i>, Anti-inflammatory, Antibacterial, 3-octanone, Antioxidant,

Abstract :

Premna mucronata Roxb. commonly known as agyon, is a plant of family Lamiaceae. This study dealt with the phytochemical analysis of leaves essential oil of Premna mucronata Roxb. (PMLO) using GC/MS technique and screening of its antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH), metal chelating and reducing power assays, anti-inflammatory activity using in-vitro albumin denaturation assay and antibacterial activity using well diffusion method against pathogenic bacterial strains Escherichia coli and Staphylococcus aureus. The phytochemical analysis of PMLO revealed the presence of over 71 compounds. 3-octanone was found to be the major constituent that accounts for 23.6% of the total oil composition. The other major constituents identified were ethyl-hexanol (13.9%), 1-octen-3-ol (9.6%), linalool (5.5%), methyl salicylate (2.9%) and E-caryophyllene (2.9%). It was found that PMLO possessed antioxidant activity in terms of DPPH (IC50=11.18±0.03 µg/mL), metal chelating (IC50=18.82±0.46 µg/mL), and reducing power activities (IC50=21.69±0.02 µg/mL) and in-vitro anti-inflammatory activity was also shown by essential oil with IC50=30.27±0.005 µg/mL. Antibacterial activity of PMLO at higher concentration (1000ppm) showed zone of inhibition of 14.33±0.58mm against E. coli and 14.66±0.58mm against S. aureus. The essential oil of leave P.mucronata contains 3-Octanone, 1-octen-3-ol, ethyl-hexanol, linalool, and have antioxidant, anti-inflammation and antibacterial acitivities.

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