Inheritance pattern and association study of agronomical traits in 47 elite accessions/genotypes of Opium poppy ( Papaver somniferum L.)

Subject Areas : Phytochemistry: Isolation, Purification, Characterization

Rashmi Lahiri ¹ , - Jyotshana ² , A.K. Verma ³ , Karuna Shanker ⁴ , Raj Kishori Lal ⁵

1 - Genetics and Plant Breeding, CSIR-Central Institute of Medicinal and Aromatic plants, Lucknow, India
2 - Department of Analytical Chemistry, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India
3 - Botanical Survey of India, Central Regional Center, Allahabad, India -211002, India
4 - Department of Analytical Chemistry, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India
5 - Genetics and Plant Breeding, CSIR-Central Institute of Medicinal and Aromatic plants, Lucknow, India

Received: 2019-11-08 Accepted : 2020-02-06 Published : 2020-03-01

Keywords: Accessions, Correlation, Path analysis, Coefficient of variation, <i>Opium poppy</i>, Seed yield,

Abstract :

Opium poppy(Papaver somniferum L.), a member of family Papaveraceae is an important source of several benzylisoquinoline derived alkaloids known to the human race since the ancient civilizations for their therapeutic and narcotic values. In the present study a total number of 47 accessions/genotypes of Opium poppywere procured from various resources and screened for economic yield, variability, heritability, correlation and path coefficient. Among them G-18 was identified as highest seed yielding 10.45 g/plant, G-3 & G-6 (0.092%, 0.086%) as high morphine containing accessions. Maximum Genotypic Coefficient of Variance (GCV) recorded for papaverine content. The estimate of heritability in the broad sense was recorded higher side for morphine content (99.96%) and papaverine (99.85 %) while lower for days to 50% flowering (4.22%). The genotypic and phenotypic correlation coefficient amongst codeine and thebaine content were found highly significant and positively associated with morphine content followed by narcotine content. The path coefficient analysis revealed that the highest direct contribution to seed yield was made by capsule straw yield (0.8730) followed by days to maturity (0.3758).

References:

Bajpai, S., Gupta, A.P., Gupta, M.M., Kumar, S., 2001. Inter-relationships between morphine and codeine in the Indian genetic resources of Opium poppy. J. Herbs Spices Med. Plants. 8(4), 75-81.

Bajpai, S., Gupta, A.P., Gupta, M.M., Sharma, S., Govil, C.M., Kumar, S., 2000. Inter-relation between descriptors and morphine yield in Asian germplasm of Opium poppy(Papaver somniferum). Gen. Res. Crop Ev. 47(3), 315-322.

Bhandari, M.M., Gupta, R., Sharma, P.P., Joshi, A., 1997. Path analysis in Opium poppy(Papaver somniferum L.). Indian J. Genet. Pl. Br. 57(1), 14-18.

Brezinova, B., Macak, M., Eftimova, J., 2009. The morphological diversity of selected traits of world collection of poppy genotypes (genus Papaver). J. Cent. Euro. Agric. 10(2), 183-192.

Demir, S., Başayiğit, L. 2019. Uzaktan Kumanda. J. Indian Soc. 47, 977.

Dewey, D.R. Lu, K., 1959. A correlation and path-coefficient analysis of components of crested wheatgrass seed production 1. Agron. J. 51(9), 515-518.

Ezeaku, I.E., Angarawai, I.I., Aladele, S.E. Mohammed, S.G., 2015. Correlation, path coefficient analysis and heritability of grain yield components in pearl millet (Pennisetum glaucum (L.) R. Br.) parental lines. J. Plant Br. Crop Sci. 7(2), 55-60.

Frick, S., Kramell, R., Kutchan, T. M., 2007. Metabolic engineering with a morphine biosynthetic P450 in Opium poppysurpasses breeding. Metab. Eng. 9(2), 169-176.

Grafius, J.E., 1959. Heterosis in Barley 1. Agron. J. 51(9), 551-4.

Gupta, M.M., Verma, R.K., 1996. Combined thin-layer chromatography-densitometry method for the quantitative estimation of major alkaloids in poppy straw samples. Indian J. Pharm. Sci. 58(4), 161.

Johnson, H.W., Robinson, H.F., Comstock, R.E., 1955. Genotypic and phenotypic correlations in soybeans and their implications in selection 1. Agron. J. 47(10), 477-483.

Kang, M.S., Miller, J.D., Tai, P.Y.P., 1983. Genetic and phenotypic path analyses and heritability in Sugarcane 1. Crop. Sci. 23(4), 643-647.

Kole, P.C., Saha, A., 2013. Studies on variability and heritability for different quantitative characters in fenugreek under different environments. J. Plant Br. Crop Sci. 5(11), 224-228.

Kumar, B., Mali, H., Gupta, E., 2014. Genetic variability, character association, and path analysis for economic traits in menthofuran rich half-sib seed progeny of Mentha piperita L. Biomed. Res. Int. 7, 1-7.

Lahiri, R., Lal, R.K., Srivastava, N., Shanker, K., 2018. Genetic variability and diversity in Indian germplasm of Opium poppy(Papaver somniferum L.). J. Appl. Res. Med. Arom. Pl. 8, 41-46.

Lal, R.K., Sharma, J.R., 1991. Genetics of alkaloids in Papaver somniferum. Planta Med. 57(3), 271-274.

Mather, K., Harrison, B.J., 1949. The manifold effect of selection. Heredity. 3(1), 1.

Mishra, B.K., Mishra, R., Jena, S.N., Shukla, S., (2016). Gene actions for yield and its attributes and their implications in the inheritance pattern over three generations in Opium poppy(Papaver somniferum L.). J. Genet. 95(3), 705-717.

Misra, H.O., Lal, R.K., Gupta, A.K., Kumar, B., Misra, A.N., Sarkar, S., Gupta, V., Singh, S., Gupta, P., Zaim, M., Singh, V.R., 2013. Genetic variability, character association and path analysis for economic traits in Bishop's weed (Ammi visnaga (L.)) Lam. Ind. Crop Prod.49, 593-597.

Muhammad, W., Khan, M.A., Nazir, M., Siddiquah, A., Mushtaq, S., Hashmi, S. S., Abbasi, B. H., 2019a. Papaver somniferum L. mediated novel bioinspired lead oxide (PbO) and iron oxide (Fe2O3) nanoparticles: In-vitro biological applications, biocompatibility, and their potential towards HepG2 cell line. Mater. Sci. Eng. C 103, 109740.

Muhammad, W., Ullah, N., Haroon, M., Abbasi, B.H., 2019b. Optical, morphological and biological analysis of zinc oxide nanoparticles (ZnO NPs) using Papaver somniferum L. RSC Adv. 9(51), 29541-29548.

Mumtaz, A., Sadaqat, H. A., Saeed, M., Yousaf, M. I., Shehzad, A., Ahmed, H.G.M.D., 2017. Genetic behavior of qualitative and seed yield-related traits in Brassica rapa. Zemdirbyste. 104(2), 147-156.

Panse V.G., Sukhatme, P.V., 1967. Statistical Methods for Agricultural Workers. Indian Council of Agricultural Research, New Delhi, India.

Saini H.C., Kaicker U.S., 1982. Model plant architecture through association and path coefficient analysis in Opium poppy. Indian J. Agric. Sci. 52, 744.

Singh, R.K. Chaudhary, B.D., 1979. Biometrical methods in Quantitative Genetic Analysis, Kalyani Publisher, New Delhi.

Singh, S.P., Shukla, S., Yadav, H.K., 2004. Genetic studies and their implication to breed desired plant type in Opium poppy(Papaver somniferum L.). Genetika 36(1), 69-81.

Verma, A.K., Singh, R.R., Singh, S., 2014. Variability in morphological characters and alkaloid profile of EMS induced mutants of Catharanthus roseus (L.) G. Don. Indian J. Genet. 74(3), 362-370.

Vir, O., Gupta, V.P., 2001. Association among yield and yield contributing characters in macrosperma x microsperma derivatives of lentil. Crop Imp. India 28(1), 75-80.

Yadav, H.K., Shukla, S., Rastogi, A., Singh, S.P., 2007. Assessment of diversity in new genetic stock of Opium poppy(Papaver somniferum). Indian J. Agric. Sci. 77(8), 537-539.

Yadav, H.K., Shukla, S., Singh, S.P., 2006. Genetic variability and interrelationship among opium and its alkaloids in Opium poppy(Papaver somniferum L.). Euphytica 150(1-2), 207-214.

Ye, K., Ke, Y., Keshava, N., Shanks, J., Kapp, J.A., Tekmal, R.R., Petros, J., Joshi, H.C., 1998. Opium alkaloid noscapine is an antitumor agent that arrests metaphase and induces apoptosis in dividing cells. Proc. Natl. Acad. Sci. 95(4), 1601-1606.