Phytochemical quantification, in vitro antioxidant and antidiabetic potentials of methanol and dichloromethane extracts of Triclisia subcordata (Oliv) leaves
Subject Areas : Phytochemistry: Isolation, Purification, CharacterizationOlubunmi Adenike Akinwunmi 1 * , Damilola Kayode Adekeye 2 , Sulaimon Adeoye Olagboye 3
1 - Department of Chemistry, Faculty of Science, Ekiti State University, PMB 5363, Ado-Ekiti, Ekiti State, Nigeria
2 - Department of Chemistry, Faculty of Science, Ekiti State University, PMB 5363, Ado-Ekiti, Ekiti State, Nigeria
3 - Department of Chemistry, Faculty of Science, Ekiti State University, PMB 5363, Ado-Ekiti, Ekiti State, Nigeria
Keywords: alfa-Amylase, phytochemicals, glucosidase, Antioxidant, <i>Triclisia subcordata</i> (Oliv), Diabetes,
Abstract :
Triclisia subcordata (Oliv) is a useful medicinal plant for the treatments of several diseases in South West, Nigeria. The methanol and dichloromethane extracts from the plant were investigated for the presence of phytochemicals, antioxidants, inhibition of α-glucosidase and α-amylase activities. The determination of the antioxidant potential of the plant extracts was achieved through evaluation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, nitric oxide scavenging activity (NOS), ferric reducing antioxidant potential (FRAP) and total phenolic content (TPC). The qualitative analysis results of the study indicated the presence of alkaloid, flavonoid, phenolics, tannin and saponin in Triclisia subcordata (Oliv) methanol extract (TRME), while only steroids, phenolics and flavonoids were detected in the Triclisia subcordata (Oliv) dichloromethane extract (TRDE). Methanol was seen to possess higher extraction capacity than dichloromethane. The quantitative determination of the extract showed that phenolics (593.7 ± 1.34) mg/100 g and flavonoids (192.6 ± 2.10) mg/100 g were more extractable in TRDE than TRME. The results showed that the plant extracts can be used to scavenge free radicals and hence, possess great antioxidant properties. It was also observed that theplantextracts possess a mild in-vitro α-glucosidase and α-amylase inhibiting activities. Thus, Triclisia subcordata (Oliv) may be used in the treatment and management of diabetes.
Abo, K.A., Lawal, I.O., Ogunkanmi, A., 2011. Evaluation of extracts of Triclisia suboardata Oliv and Heinsia crinita (Afz) G. Taylor for antimicrobial activity against some clinical bacterial isolates and fungi.Afr. J. Pharm. Pharmacol. 5(2), 125-131.
Allam E.A., Alaa, M.N., Amgad, I.M.K., Toshinori, N., Kuniyoshi, D., 2018. Potential activities for constituents from Vicia faba L.Trends Phytochem. Res. 2(1), 21-26.
Asuzu, I.U, Anaga, A.O., 1995. The anti-ulcer effect of methanolic extract of Triclisia subcordata O. leaves in rats. J. Herbs Spices Med. Plants 3(3), 45-53.
Ayoola, M.D., Akinwunmi, K.F., Agboola, O.B., 2017. Anti-diabetic and antioxidant activities of Entandrophragma cylindricum and Triclisia subcordata. Niger J. Nat. Prod. Med. 21(1), 24-31.
Benzie, I.F.F., Strain, J.J., 1999. Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol.299, 15-27.
Boukhanouf, S., Louaileche, H., Perrin, D., 2016. Phytochemical content and in vitro antioxidant activity of Faba bean (V. faba L.) as affected by maturity stage and cooking practice. Int. Food Res. J. 23(3), 954-961.
Bozin, B., Mimica-Dukic, N., Bogavac, M., Suvajdzic, L., Simin, N., Samojlik, I., Couladis, M., 2008. Chemical composition, antioxidant and antibacterial properties of Achiliea becker ex Heimerl S.I. and A. pannonica Scheele essential oils. Molecules 13(9), 2058.
Brunner, J.H., 1984. Direct spectrophotometer determination of saponin. Anal. Chem. 34(396), 1314-1326.
Camilo, C.J., Alves Nonato, C.d.F., Galvão-Rodrigues, F.F., Costa, W.D., Clemente, G.G., Sobreira Macedo, M.A.C., Galvão Rodrigues, F.F., da Costa, J.G.M., 2017. Acaricidal activity of essential oils: a review. Trends Phytochem. Res. 1(4), 183-198.
Daziel, J.M., 1937. The Useful Plants of West Tropical Africa. The Crown Agents for the Colonies, London.
Dey, T.B., Chakraborty, S., Jain, K.K., Sharma, A., Kuhad, R.C., 2016. Antioxidant phenolics and their microbial production by submerged and solid state fermentation process: A review. Trends Food Sci. Technol. 53, 60-74.
Dhakal, S., Aryal, P., Aryal, S., Bashyal D., Khadka, D., 2016. Phytochemical and antioxidant studies of methanol and chloroform extract from leaves of Azadirachta indica A.Juss. in tropical region of Nepal. J. Pharmacognosy Phytother. 8(12), 203-208.
Dib, I., Tits, M., Angenot, L., Wauters, J.N., Assaidi, A., Mekhfi, MH., Aziz, M., Bnouham, M., Legssyer, A., Frederich, M., Ziyyat, A., 2017. Antihypertensive and vasorelaxant effects of aqueous extract of Artemisia campestris L. from Eastern Morocco. J. Ethnopharmacol. 206, 224-235.
Du, G., Sun, L., Zhao, R., Du, L., Song, J., Zhang, L., He, G., Zhang, Y., Zhang, J., 2016. Polyphenols: Potential source of drugs for the treatment of ischaemic heart disease. Pharmacol. Ther. 162, 23-34.
Du, L., Shen, Y., Zhang, X., Prinyawiwatkul, W., Xu, Z., 2014. Antioxidant-rich phytochemicals in miracle berry (Synsepalum dulcificum) and antioxidant activity of its extracts. Food Chem. 153, 279-284.
Fridovich, I., 1986. Biological effects of the superoxide radical. Arch. Biochem. Biophys. 247(1), 1-11.
Garat, D.C., 1964. The Quantitative Analysis of Drugs, Chapman and Hall, Tokyo, Japan,
Hodzic, Z., Pasalic, H., Memisevic, A., Scrabovic, M., Saletovic, M. Poljakovic, M., 2009. The influence of total phenols content on antioxidant capacity in the whole grain extracts. Eur. J. Sci. Res. 28, 471-477.
Harbone, J.B., 1973. Phytochemical Methods, a Guide to Modern Technique of Plant Analysis. Chapman and Hall, London.
http://www.waiwiki.org/index.php?title=File:Triclisia_subcordata_2.jpg, accessed on line on 5/11/2019.
Huang, D., Ou, B., Prior, R.L., 2005. The chemistry behind antioxidant capacity assays. J.Agric. Food Chem. 53(6), 1841-1856.
Indumathi, C., Durgendevi, G., Nathyavani, S., Gayathri, K., 2014. Estimation of terpenoid content and its antimicrobial property in Enicostemma litorrale. Int. J. Chemtech. Res. 6(9), 4264-4267.
Irvine, F.R., 1961. “Woody Plants of Ghana”, Oxford University Press, London.
Keerthana, G., Kalaivani, M.K., Sumathy, A., 2013. In-vitro alpha amylase inhibitory and anti-oxidant activities of ethanolic extract of croton bonplandianum.Asian J. Pharm. Clin. Res. 6(4), 32-36.
Kim, S.K., Jung, S.M., Ahn, K.H., Jeon, H.J., Lee, D.H., Jung, K.M., Jung, S.Y., Kim, D.K., 2005. Identification of three competitive inhibitors for membrane-associated, Mg2+-dependent and neutral 60kDa sphingomyelinase activity. Arch. Pharm. Bull. 28(4), 1300-1303.
Li, W., 2016. Cytotoxicity effects and apoptosis induction by cycleanine and tetrandrine. Planta Medica: Nat. Prod. Med. Plant Res. 30(9), 1533-1539.
Mahdavi, B., 2017. Chemical compositions of essential oils from Etlingera brevilabrum: A comparative analysis using GC×GC/TOFMS. Trends Phytochem. Res. 1(1), 15-22.
Mbahi, M.A., Mbahi, A.M., Umar, I.A., Ameh, D.A., Joseph, I., Amos, P.I., 2018. Phytochemical screening and antimicrobial activity of the pulp extract and fractions of Ziziphus mauritiana. Biochem. Anal. Biochem. 7(2), 1-6.
Moss, G.P., 1989. The Working Party of the IUPAC-IUB Joint Commission on Biochemical Nonmenclature. “Nomenclature of steroids, recommendations 1989”. Pure Appl. Chem. 61(10), 1783-1822.
Mohadjerani, M., Asadollahi, A., 2019. Veronica crista-galli Steven and Veronica persica Poir. as anticancer and antioxidant plants in-vitro. Trends Phytochem. Res. 3(1), 61-66.
Mohammadhosseini, M., Venditti, A., Sarker, S.D., Nahar, L., Akbarzadeh, A., 2019. The genus Ferula: Ethnobotany, phytochemistry and bioactivities-A review. Ind. Crop Prod. 129, 350-394.
Mohammadhosseini, M., Sarker, S.D., Akbarzadeh, A., 2017. Chemical composition of the essential oils and extracts of Achillea species and their biological activities: A review. J. Ethnopharmacol. 199, 257-315.
Mohammadhosseini, M., 2017. The ethnobotanical, phytochemical and pharmacological properties and medicinal applications of essential oils and extracts of different Ziziphora species. Ind. Crop Prod. 105, 164-192.
Oladosu-Ajayi, R.N., Dienye, H.E.; Ajayi, C.T., Agha, I.U., 2016. Vernonia amygdalina (Bitter leaf) extracts as preservative for catfish, Clarias gariepinus. IJNF. 3(1), 102-108.
Posedek, A., 2007. Natural antioxidants and antioxidant capacity of Brassica vegetables: a review. SOSSTA. 40, 1-11.
Rabeta, M.S., Nur, F.R., 2013. Total phenolic content and ferric reducing antioxidant power of the leaves and fruits of Garcinia atrovirdis and Cynometra cauliflora.Int. Food Res. J. 20(4),1691-1696.
Sarker, S.D., Nahar, L., 2018. Evidence-based phytotherapy: what, why and how? Trends Phytochem. Res. 2(3), 125-126.
Sellappan, S., Akoh, C.C., Krewer, G., 2002. Phenolic compounds and antioxidant capacity of georgia-grown blueberries and blackberries.J. Agric. Food Chem 50(8), 2432-2438.
Singh, R., 2015. Medicinal plants: A review. J. Plant Sci. Special Issue: Medicinal Plants. 3(1), 50-55.
Singleton, V.L., Rossi, J.A., 1956. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticul. 16(3), 144-158.
Sofowora, A., 1993. Phytochemical screening of medicinal plants and traditional medicine in Africa 2nd Edition Spectrum Books Limited, Nigeria.
Tiwari, P., Kumar, B., Kaur, M., Kaur, G., Kaur, H., 2011. Phytochemical screening and extraction: A review. IPS 1(1), 98-106.
Trease, G.E., Evans, W.C., 1989. Textbook of Pharmacognosy. 12th Ed. Balliere, Tindall, London, UK.
Trease, G., Evans S.M., 1993. Medicinal Plants and Traditional Medicines in Africa, 2nd Ed., Spectrum Books.
Uche, F.I., Drijfhout, F.P., McCullagh, J., Richardson, A., Li, W.W. (2016). Cytotoxicity effects and apoptosis induction by bisbenzylisoquinoline alkaloids from Triclisia subcordata. Phytother. Res. 30(9), 1533-1539.
Uche, F.I., Abed, M.N., Abdullah, M.I., Drijfhout, F.P., McCullagh, J., Claridge, T.W., Richardson, A., Li, W.W., 2017. Isochondodendrine and 2′-norcocsuline: additional alkaloids from Triclisia subcordata induce cytotoxicity and apoptosis in ovarian cancer cell lines. RSC Adv. 7(70), 44154-44161.
Xiao, Z., Storms, R., Tsang, A., 2006. A quatitative starch-iodine method for measuring alpha amylase and glucoamylase activities. Anal. Biochem. 351(1), 146-148.