مقایسه تاثیر تمرینات مقاومتی، استقامتی و ترکیبی بر بیان ژن آدیپونکتین در بافت‌های ماهیچه دست و پای موش های نر نژاد ویستار
تاثیر تمرینات مقاومتی، استقامتی و ترکیبی بر بیان ژن آدیپونکتین در بافت‌های ماهیچه
محورهای موضوعی : تشخیص مولکولی نشانگر های بیوشیمیایی و ژنتیکی
zahra malekian
1
,
طاهره باقرپور
2
,
نعمت اله نعمتی
3
1 - دانشجوی دکتری، گروه تربیت بدنی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
2 - استادیار گروه تربیت بدنی،واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
3 - دانشیار گروه تربیت بدنی،واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
کلید واژه: آدیپونکتین, تمرین ترکیبی, تمرین استقامتی و تمرین مقاومتی,
چکیده مقاله :
زمینه و هدف : آدیپونکتین نوعی آدیپوکاین است که در متابولیسم قند و لیپید ها نقش دارد، این آدیپوکاین علاوه بر بافت چربی توسط بافت های دیگر مانند عضلات و کبد نیز ترشح می شود. مطالعه حاضر با هدف بررسی مقایسه اثر تمرینات استقامتی، مقاومتی و ترکیبی بر سطح بیان ژن آدیپونکتین در ماهیچه‌های دست و پای موش‌های صحرایی نر نژاد ویستار طراحی شد. مواد و روش¬ها : مطالعه حاضر پژوهشی تجربی چندگروهی است که در آن 40 سر موش نر سالم نژاد ویستار در چهار گروه ده‌تایی کنترل، تمرین مقاومتی، استقامتی و ترکیبی تقسیم شدند. هر گروه به مدت 8 هفته تمرین‌های مختص خود را انجام دادند. در نهایت ماهیچه دست و پا برای اندازه گیری ژن آدیپونکتین مورد بررسی قرار گرفتند. نتایج : نتایج آنالیزها نشان داد که میزان بیان ژن آدیپونکتین در بین تمام گروه‌ها با هم تفاوت داشت (001/0 > P). میزان بیان نسبی ژن آدیپونکتین در تمام گروه‌های تمرینی نسبت به گروه کنترل افزایش معناداری داشت (001/0 > P). همچنین، در مقایسه گروه‌های تمرینی با هم، تمرین‌های ترکیبی بیشترین تاثیر و تمرین مقاومتی کمترین تاثیر را اعمال کردند. در مقایسه درون گروهی، بیان ژن آدیپونکتین در تمرین مقاومتی در ماهیچه دست بیشتر از پا بود (047/0 = P)، اما در تمرین استقامتی بیان ژن در ماهیچه پا بیشتر بود (029/0 = P). این دو ماهیچه در هنگام تمرین ترکیبی با هم تفاوت معناداری نداشتند (086/0 = P). نتیجه گیری : بر اساس نتایج این مطالعه، تمام شیوه‌های تمرین‌های ورزشی می‌توانند بیان ژن آدیپونکتین را افزایش دهند، اما تمرین ترکیبی، استقامتی و مقاومتی به ترتیب بیشترین تاثیر را دارند.
Background & Aim: Adiponectin is a type of adipokine that is involved in sugar and lipid metabolism, this adipokine is in addition to the tissue that it is like and can become. The present study was designed to compare the effect of endurance, resistance and combined exercises on the level of adiponectin gene expression in the hand and leg muscles of Wistar rats. Materials & methods: The current study is an experimental research in which 40 healthy male Wistar rats were divided into four ten groups of control, resistance training, endurance and combined. Each group did their own exercises for 8 weeks. Finally, hand and foot muscles were examined to measure the adiponectin gene. Results: The results of the analyzes showed that the level of adiponectin gene expression was different among all groups (P < 0.001). The relative expression of adiponectin gene in all training groups increased significantly compared to the control group (P < 0.001).Also, comparing exercise groups together, combined exercises had the greatest effect and resistance exercise had the least effect. In intra-group comparison, adiponectin gene expression was higher in hand muscle than leg muscle during resistance training (P = 0.047), but gene expression was higher in leg muscle during endurance training (P = 0.029). These two muscles were not significantly different during combined training (P = 0.086). Conclusion: According to the results of this study, all types of sports training can increase the expression of adiponectin gene, but combined, endurance and resistance training have the greatest effect, respectively.
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