ارزیابی تغییرات فیزیولوژیکی و بیوشیمیایی توت‌فرنگی رقم گاویوتا در پاسخ به قارچ‌های میکوریزا و تریکودرما تحت تیمار سلنیوم

محورهای موضوعی : فصلنامه کیفیت و ماندگاری تولیدات کشاورزی و مواد غذایی

علی لچینانی ¹ , سید جلال طباطبایی ² , امیر بستانی ³ , وحید عبدوسی ⁴ , سعید رضائی ⁵

1 - دانشجوی دکتری تخصصی، گروه علوم باغبانی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - استاد، گروه علوم باغبانی، دانشکده علوم کشاورزی، دانشگاه شاهد ، تهران، ایران
3 - دانشیار، گروه خاک شناسی، دانشکده علوم کشاورزی، دانشگاه شاهد، تهران، ایران
4 - استادیار، گروه علوم باغبانی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
5 - استادیار، گروه گیاهپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

تاریخ دریافت : 1402/02/17 تاریخ پذیرش : 1402/04/20 تاریخ انتشار : 1402/06/21

کلید واژه: قارچ‌های همزیست, کلروفیل, ظرفیت آنتی‌اکسیدانی, پروتئین, آنتوسیانین,

چکیده مقاله :

بررسی حاضر اثر قارچ‌های میکوریزا (Rhizophagus irregularis)، تریکودرما (Trichoderma harzianum) و سلنیوم بر برخی صفات فیزیولوژیکی توت‌فرنگی رقم گاویوتا در آزمایشی به‌صورت فاکتوریل در قالب طرح پایه کاملاً تصادفی در سه تکرار در گلخانه تحقیقاتی دانشکده علوم کشاورزی دانشگاه شاهد در سال 1396 را مورد ارزیابی قرار گرفت. تیمارها شامل قارچ (بودن تلقیح، میکوریزا، تریکودرما) و سلنیوم (0، 5/0، 1، 2 و 4 میلی‌گرم بر کیلوگرم خاک) بود. نتایج نشان داد که استفاده از قارچ‌های همزیست و به ویژه تریکودرما توانست به طور معنی‌داری باعث افزایش میزان pH، مواد جامد محلول کل (TSS)، ظرفیت آنتی‌اکسیدانی و آنتوسیانین میوه و نیز افزایش میزان پروتئین، کلروفیل و کاروتنوئید برگ توت‌فرنگی رقم گاویوتا گردد. همچنین تیمار سلنیوم به ویژه در غلظت‌های کم ( 5/0 و 1 میلی‌گرم بر کیلوگرم خاک) توانست ظرفیت آنتی‌اکسیدانی و آنتوسیانین میوه و همچنین میزان پروتئین و کلروفیل برگ را افزایش دهد. در نهایت می‌توان استفاده از قارچ‌های همزیست به ویژه تریکودرما را در غلظت‌های کم سلنیوم ( 5/0 و 1 میلی‌گرم بر کیلوگرم خاک) در برنامه‌های کشت توت‌فرنگی رقم گاویوتا توصیه نمود.

چکیده انگلیسی:

This study evaluated the effects of Mycorrhiza (Rhizophagus irregularis), Trichoderma (Trichoderma harzianum) and selenium on some physiological traits of Gaviota strawberry as a factorial experiment based on a completely randomized design with three replications in the Shahed University, Faculty of Agricultural Sciences research greenhouse in 2017. The treatments included fungi (No inoculation, Mycorrhiza, Trichoderma) and selenium (0, 0.5, 1, 2 and 4 mg/kg soil). The results showed that the use of symbiotic fungi, especially Trichoderma, could significantly increase the pH, total soluble solids (TSS), antioxidant capacity and anthocyanin of the fruit, as well as increase the amount of protein, chlorophyll and carotenoid of Gaviota strawberry leaves. Furthermore, selenium treatment, especially at low concentrations (0.5 and 1 mg/kg soil), could increase the antioxidant and anthocyanin capacity of the fruit, as well as the amount of protein and chlorophyll in the leaves. Overall, it is possible to recommend the using of symbiotic fungi, especially Trichoderma, along with low concentrations of selenium (0.5 and 1 mg/kg soil) in Gaviota strawberry cultivation programs.

منابع و مأخذ:

1- Giampieri F, Forbes-Hernandez TY, Ga-sparrini M, Alvarez-Suarez JM, Afrin S, Bompadre S, Quiles JL. Mezzetti B, Battino M. Strawberry as a health promoter: an evidence based review. Food & Function. 2015; 6(5): 1386-1398.

2- Afrin S, Gasparrini M, Forbes-Hernan-dez TY, Reboredo-Rodriguez P, Mezzetti B, Varela-López A, Giampieri F, Battino M. Promising health benefits of the stra-wberry: A focus on clinical studies. Journal of Agricultural and Food Chemistry. 2016; 64(22): 4435-4449.

3- Hummerr KE, Hancock J. Strawberry genomics: Botanical history, cultivation, traditional breeding, and new technologies. In: Folta K, Gardiner S. editors. Genetics and Genomics of Rosaceae. Springer Scie-nce + Business Media, New York, 2009; pp. 413-435.

4- Hummerr KE, Bassil N, Njuguna W. Fragaria. In: Kole C. editor. Wild Crop Re-latives: Genomic and Breeding Resources, Temperate Fruits. 2011; pp. 17-43.

5- Tan H, Barret M, Rice O, Dowling DN, Burke J, Morrissey JP, O’Gara F. Long-term agrichemical use leads to alterations in bacterial community diversity. Plant, Soil and Environment. 2012; 58: 452-458.

6- Pahalvi HN, Rafiya L, Rashid S, Nisar B, Kamili AN. Chemical Fertilizers and Their Impact on Soil Health. In: Dar GH, Bhat RA, Mehmood MA, Hakeem KR. editors. Microbiota and Biofertilizers, Springer, Cham. 2021.

7- Bharti N, Suryavanshi M. Quality control and regulations of biofertilizers: Current scenario and future prospects. In: Rakshit A, Meena VS, Parihar M, Singh HB, Singh AK. editors. Biofertilizers, Woodhead Pub-lishing. 2021; pp. 133-141.

8- Mitter EK, Tosi M, Obregón D, Dunfield KE, Germida JJ. Rethinking crop nutrition in times of modern microbiology: Innov-ative biofertilizer technologies. Frontiers in Sustainable Food Systems. 2021; 5: 606-815.

9- Van der Heijden MGA, Martin FM, Sel-osse MA, Sanders IR. Mycorrhizal ecology and evolution: the past, the present, and the future. New Phytologist. 2015; 205(4): 1406-1423.

10- Singh D, Thapa S, Geat N, Mehriya ML, Rajawat MVS. Biofertilizers: Mechan-isms and application. In: Rakshit A, Meena VS, Parihar M, Singh HB, Singh AK. editors. Biofertilizers, Woodhead Publish-ing. 2021; pp. 151-166.

11- Figueiredo AF, Boy J, Guggenberger G. Common mycorrhizae network: A review of the theories and mechanisms behind und-erground interactions. Frontiers in Fungal Biology. 2021; 2: 735299.

12- Salehi H, Javadi T, Ghaderi N. The effect of mycorrhiza on the quality charact-eristics of strawberry fruit in different cult-ure environments in soilless culture. The 12th Congress of Horticultural Sciences of Iran. 2021; 5 Sep, Rafsanjan, Iran. [In Persian]

13- Tyśkiewicz R, Nowak A, Ozimek E, Jaroszuk-Ściseł J. Trichoderma: The curr-ent status of its application in agriculture for the biocontrol of fungal phytopathogens and stimulation of plant growth. Interna-tional Journal of Molecular Sciences. 2022; 23(4): 2329.

14- Kumar S, Diksha L, Sindhu SS, Kumar R. Biofertilizers: An ecofriendly techno-logy for nutrient recycling and environm-ental sustainability. Current Research in Microbial Sciences. 2022; 3: 100094.

15- Schiavon M, Lima LW, Jiang Y, Haw-kesford MJ. Effects of Selenium on Plant Metabolism and Implications for Crops and Consumers. In: Pilon-Smits E, Winkel L, Lin ZQ. editors. Selenium in Plants. Plant Ecophysiology, Springer, Cham. 2017.

16- Lanza MGDB, Reis AR. Roles of selenium in mineral plant nutrition: ROS scavenging responses against abiotic stre-sses. Plant Physiology and Biochemistry. 2021; 164: 27-43.

17- Zahedi SM, Abdelrahman M, Sadat Hosseini M, Fahadi Hoveizeh N, Tran LSP. Alleviation of the effect of salinity on growth and yield of strawberry by foliar spray of selenium-nanoparticles. Environm-ental Pollution. 2019; 253: 246-258.

18- Narváez-Ortiz WA, Martínez-Hernán-dez M, Fuentes-Lara LO, Benavides-Men-doza A, Valenzuela-García JR, González-Fuentes JA. Effect of selenium application on mineral macro- and micronutrients and antioxidant status in strawberries. Journal of Applied Botany and Food Quality. 2018; 91: 321-331.

19- Javaherdashti M, Ghasemnezhad M, Smizadeh Lahiji H, Shiri MA. Comparison of nutritional value and antioxidant compo-unds of Some winter pumpkin (Cucurbita Sp) species fruits in Iran. Advances in Envi-ronmental Biology. 2012; 6(10): 2611-2616

20- Brand-Williams, W, Cuvelier ME, Ber-set C. Use of free radical method to evaluate antioxidant activity. LWT-Food Science Technology. 1995; 28(1): 25-30.

21- Ghasemnezhad M, Zareh S, Shiri MA, Javdani J. The arils characterization of five different pomegranate (Punica granatum) genotypes stored after minimal processing technology. Journal of Food Science and Technology. 2015; 52(4): 2023-2032. 

22- Shiri MA, Ghasemnezhad M, Fattahi Moghaddam J, Ebrahimi R. Enhancing and maintaining nutritional quality and bioa-ctive compounds of ‘Hayward’ kiwifruit: Comparison the effectiveness of different CaCl₂ spraying times. Journal of Food Processing and Preservation. 2016b; 40(5): 850-862.

• 23- Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Bioche-mistry. 1976; 72(1-2): 248-54.


• 24- Duc NH, Mayer Z, Pek Z, Helyes L, Posta K. Combined inoculation of arbuscu-lar mycorrhizal fungi, Pseudomonas fluor-escens and trichoderma For enhancing defense enzymes and yield of three pepper cultivars. Applied Ecology and Environm-ental Research. 2017; 15(3): 1815-1829.


• 25- Yuan S, Li M, Fang Z, Liu Y, Shi W, Pan B, Shen Q. Biological control of tob-acco bacterial wilt using Trichoderma har-zianum amended bioorganic fertilizer and the arbuscular mycorrhizal fungi Glomus mosseae. Biological Control. 2016; 92: 164-171.


• 26- Sarkar J, Ray A, Chakraborty B, Cha-kraborty U. Antioxidative changes in Citrus reticulata induced by drought stress and its effect on root colonization by arbuscular mycorrhizal fungi. European Journal of Biological Research. 2016; 6(1): 1-13.

27- Zahangeer Alam M, McGee R, Anamul Hosque M, Jalal Ahamad G, Carpenter-Boggs L. Effect of arbuscular mycorrhizal fungi, selenium and biochar on pho-tosynthetic pigments and aqntioxidant enzyme activity under arsenic stress in Mung Bean (Vigna radiata). Physiologist. 2019; 10: 193:1-13.

• 28- Beladel B, Nedjimi B, Mansouri A, Tahtat D, Belamri M, Tchanchane A, Kh-elfaoui F, Benamar MEA. Selenium content in wheat and estimation of the selenium daily intake in different regions of Algeria. Applied Radiation and Isotopes. 2013; 71: 7-10.


• 29- Pu Z, Wei G, Liu Z, Chen L, Guo H, Li Y, Li Y, Dai S, Wang J, Li W, Jiang Q, Wei Y, Zheng Y. Selenium and anthocyanins share the same transcription factors R2R3-MYB and bHLH in wheat. Food Chemistry, 2021; 356; 129699.

30- Pilon-Smits EAH, Winkel LHE, Lin ZQ. Selenium in Plants: Molecular, Phy-siological, Ecological and Evolutionary Aspects. Plant Ecophysiology. 2017; pp. 324.

31- Bolandnazar SA, Neyshaboury MR, Aliasgharzad N, Chapazadeh N. Effects of mycorrhizal colonization on growth param-eters of onion under dofferent irrigation and soil coditions. Pakistan Journal of Biology Sciences. 2007; 10(9): 1491-1495.

32- Asensio D, Rapparini F, Peñuelas J. AM fungi root colonization increases the prod-uction of essential isoprenoids vs none-ssential isoprenoids especially under droug-ht stress conditions or after jasmonic acid application. Phytochemistry. 2012; 77: 149-161.

33- Johnson D, Leake JR, Ostle N, Ineson P, Read DJ. In situ 13CO₂ pulselabelling of upland grassland demonstrates a rapid path-way of carbon flux from arbuscular mycorr-hizal mycelia to the soil. New Phytologist. 2002; 153: 327-334.

34- Begum N, Qin C, Ahanger MA, Raza S, Khan MI, Ashraf M, Zhang L. Role of arbu-scular mycorrhizal fungi in plant growth regulation: Implications in abiotic stress tolerance. Frontiers in Plant Science. 2019; 10. 

35- Feng RW, Wei CY. Antioxidative me-chanisms on selenium accumulation in Pteris vittata L., a potential selenium phy-toremediation plant. Plant Soil and Environment. 2012; 58, 105-110.

• 36- Pennanen A, Hartikainen H, Lukkari K, Ollilainen V. Acclimation of Lactuca sativa to increased UV irradiation at various selenium levels. Photosynthesis Research. 2001; 69: 30.


• 37- Namdar M, Arvin MJ, Bahremand N. Effect of selenium on growth, physiological and biochemical indices of garlic plant (Allium sativum) under cadmium toxicity. Plant Process and Function. 2019; 8(30): 137-153. [In Persian]


• 38- Hawrylak-Nowak B. Enhanced seleni-um content in sweet basil (Ocimum basili-cum ) by foliar fertilization. Vegetable Crops Research Bulletin. 2008; 69: 63-72.

39- Mohammadi Kashka F, Pirdashti H, Ya-ghoubian Y. Inoculation with Trichoderma virens and Piriformospora indica for im-proving the morphological and physiologi-cal traits related to grain yield of rice under different rates of phosphorus fertilizer. Journal of Crop Ecophysiology. 2018; 11(4): 857-874. [In Persian]

 

40- Shekari L, Kamelmanesh M, Mozafari-yan M, Hasanuzzaman M, Sadeghi F. Role of selenium in mitigation of cadmium tox-icity in pepper grown in hydroponic con-dition. Journal of Plant Nutrition. 2017; 40(6): 761-772.