شناسایی مولکولی گونه های کمپیلوباکتر و ارزیابی پتانسیل بیماریزایی این جدایه ها در گاو، بر اساس وجود ژن های سم کشنده تورمی سلولی CDT
محورهای موضوعی : میکروب شناسی مولکولیسیده ام البنین قاسمیان 1 , حمید محمودی پور 2
1 - استادیار، گروه دامپزشکی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران.
2 - استادیار، گروه پرستاری و مامایی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران.
کلید واژه: سم متسع کننده سلولی, کمپیلوباکتر ژژونی, کمپیلوباکتر کلی, گاو.,
چکیده مقاله :
سابقه و هدف: بسیاری از باکتری‌های گرم منفی توانایی تولید سم کشنده تورمی سلولی را دارند. این سم از سه زیرواحد کدگذاری شده توسط ژن‌های cdtA، cdtB و cdtC ساخته شده است. هدف از این مطالعه تعیین شیوع ژن‌های cdtA، cdtB و cdtC در کمپیلوباکتر ژژونی و کمپیلوباکتر کلی جدا شده از گاو میباشد. مواد و روشها: در این مطالعه مقطعی، 152 نمونه مدفوع گاو جمع آوری شد. همه نمونهها برای جداسازی گونه‌های کمپیلوباکتر با استفاده از روش prêt KB مورد آزمایش قرار گرفتند. سپس نمونه‌های مشکوک کمپیلوباکتر با استفاده از روش توالی‌یابی DNA ژن‌های 16SrRNA احراز هویت شدند. در نهایت، جدایه‌های کمپیلوباکتر برای تشخیص ژن‌های cdtA، cdtB و cdtC مورد ارزیابی قرار گرفتند. یافته‌ها: از میان 152 نمونه 14 نمونه (20/9%) مدفوع گاو از نظر کمپیلوباکتر مثبت بودند. از این تعداد 9 نمونه (30/64%) به عنوان کمپیلوباکتر ژژونی و 5 نمونه (70/35%) به عنوان کمپیلوباکتر کلی تشخیص داده شدند. در سنجش توسط PCR شیوع ژن‌های حدت cdtA، cdtB و cdtC در گاو به ترتیب 29/64، 71/85، 71/85 درصد بود. این دادهها نشان میدهد که ژن‌های cdtA، cdtB و cdtC در بین جدایه‌های کمپیلوباکتر گسترده هستند. تجزیه و تحلیل آماری نشان داد که بین بروز ژن‌های cdtA، cdtB و cdtC و گونه‌های کمپیلوباکتر با نوع میزبان ارتباط معنی داری وجود ندارد. نتیجهگیری: نتایج نشان ميدهد كه اكثر اين جدايهها سمCDT را در سطح و مقدار زياد توليد كردند. بنابراین، سویههای کمپیلوباکتر جدا شده از گاو میتواند عامل عفونی برای انسان در نظر گرفته شود.
Background & Objectives: Many Gram-negative bacteria have the ability to produce cell swelling toxins, which are composed of three subunits encoded by the cdtA, cdtB, and cdtC genes. The aim of this study was to determine the prevalence of cdtA, cdtB, and cdtC in Campylobacter jejuni and Campylobacter coli isolated from cattle. Materials & Methods: In this cross-sectional study, 152 stool samples were collected from cows. All samples were tested for the isolation of Campylobacter species using the prêt KB method. Then, suspected Campylobacter samples were authenticated using DNA sequencing method of 16SrRNA genes. Finally, Campylobacter isolates were evaluated for detection of cdtA, cdtB and cdtC genes. Results: Of the 152 samples, 14 (9.20%) of cow feces were positive for Campylobacter spp. Of these, nine samples (64.30%) were diagnosed with Campylobacter jejuni and five samples (35.70%) were diagnosed with Campylobacter coli. The prevalence of the cdtA, cdtB, and cdtC virulence genes in cattle was 64.29, 85.71, and 85.71%, respectively. These data show that cdtA, cdtB, and cdtC are widespread among Campylobacter isolates. Statistical analysis showed that there was no significant relationship between the occurrence of cdtA, cdtB, and cdtC and Campylobacter species with the host type. Conclusion: Resuls shows that most of these isolates produced CDT toxin at a high level and quantity. Therefore, Campylobacter strains isolated from cattle can be considered infectious agents for humans.
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