بررسی اثر فاکتور رشد در استحکام تاندون همراه با تابش پلاسما در خرگوش
محورهای موضوعی :
علیرضا عمرانی فرد
1
,
علیرضا جهاندیده
2
*
,
احمد اصغری
3
,
سعید حصارکی
4
1 - گروه علوم درمانگاهی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه علوم درمانگاهی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
3 - گروه علوم درمانگاهی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
4 - گروه پاتوبیولوژی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
کلید واژه: ترمیم تاندون, پلاسمای سرد اتمسفری, فاکتور دو رشد فیبروبلاست, بافت شناسی, کشش تاندون,
چکیده مقاله :
امروزه درمان تاندون آسیب دیده و بازیابی عملکرد آن در انسان و حیوانات از چالش های پزشکی است . هدف: بررسی درمان همزمان فاکتور دو رشد فیبروبلاست (bFGF) و پلاسمای سرد اتمسفریک (CAP) بر استحکام کششی تاندون با نقیصه تجربی آشیل خرگوش انجام شد. روش کار : در 20 خرگوش تحت جراحی نقیصه تجربی تاندونی مشابه ایجاد شد بعد از بخیه پوست به چهار گروه تقسیم شدند ، در گروه اول یا کنترل آزمایش; 0.2(mL) آب مقطر در بالا و پایین محل آسیب تزریق شد. در گروه دوم یا درمان مستقل با ;bFGF محلول حاوی 10(µg) از bFGF در بالا و پایین ضایعه تزریق شد. در گروه سوم درمان مستقل ;CAP تاندون آشیل طی 21 روز، روزانه به مدت 30 ثانیه در معرض CAP از روی سطح پوست قرار گرفت. گروه چهارم یا درمان ترکیبی CAP)+(bFGF ;بطور همزمان تحت درمان با CAP و bFGF قرارگرفتند. یافته ها : نتایج نشان داد گروه CAP+bFGF نسبت به گروه کنترل و گروه‌های درمان مستقل از لحاظ بافت شناسی پیشرفت ترمیمی قابل توجهی دارد (05/0 >P). آزمون کشش با تحمل نیروی (04/125N) نیوتن مقاومت بالاتری نسبت به سایر گروه‌های آزمایش نشان داد (001/0>P). نتیجه گیری: به نظر میرسد CAP و bFGF به تنهایی تاثیر مشابه و مثبتی بر روند ترمیم و استحکام تاندون دارند ولی استفاده همزمان آنها منجر به تقویت اثر آنها بر فرایند التیام و ساختار تاندون ترمیم شده و در نهایت باعث افزایش قابل توجه استقامت تاندون در برابر کشش میشود.
Nowadays, treatment of tendons injury and restoring their function in humans and animals is one of the medical challenges. This study investigating effect of simultaneous treatment of fibroblast growth factor-2 (bFGF) and cold atmospheric plasma (CAP) on injured Achilles tendon in rabbits in order to evaluate effect on tensile tolerance and histological quality of repaired tendon tissue. For this purpose. In the first experiment group 0.2 ml distilled water injected proximal and distal of injury site for control. The second group bFGF was performed according to the first experiment, with the difference that (10 µg) bFGF in 0.2 ml of distilled water was injected. In the third group CAP Achillis tendon expose to CAP from the skin surface for 30 seconds for 21 consecutive days. fourth group (CAP–bFGF) were treated both CAP and bFGF. According to the obtained results after 120 days simultaneous treatment (CAP–bFGF) group showed significant histological improvement compared to the control group ( P< 0.001 ) and independent treatment groups (P < 0.05). also, it showed higher endurance in tensile test with average bearing force of (125.04 N) compared to other test groups ( P< 0.001 ) Finally, the analysis of the obtained results showed that CAP and bFGF independently improve the healing and strength of tendon, but no significant difference was seen between their effectiveness. whereas simultaneous use of fibroblast growth factor and atmospheric cold plasma radiation leads to enhancement of the therapeutic effect and tendon tensile strength as compared to independent treatments.
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