اثر ازتوباکتر و سطوح کود شیمیایی نیتروژن روی صفات آگروفیزیولوژیک و عملکرد ژنوتیپ‌های گلرنگ (Carthamus tinctorius L.) در شرایط متفاوت رطوبتی

الموضوعات : اکوفیزیولوژی گیاهان زراعی

عباس سلیمانی فرد ¹ (گروه زراعت، پردیس علوم و تحقیقات خوزستان، دانشگاه آزاد اسلامی، اهواز، ایران. گروه زراعت، واحد اهواز، دانشگاه آزاداسلامی، اهواز، ایران)
مانی مجدم ² (گروه زراعت، واحد اهواز، دانشگاه آزاداسلامی، اهواز، ایران)
شهرام لک ³ (گروه زراعت، واحد اهواز، دانشگاه آزاداسلامی، اهواز، ایران)
مجتبی علوی‌فاضل ⁴ (گروه زراعت، واحد اهواز، دانشگاه آزاداسلامی، اهواز، ایران)

تاريخ الإرسال : 16 الأربعاء , ربيع الأول, 1441 تاريخ التأكيد : 01 الخميس , محرم, 1442 تاريخ الإصدار : 18 الجمعة , جمادى الثانية, 1443

الکلمات المفتاحية: تنش خشکی, عملکرد دانه, کلروفیل, تعداد غوزه در بوته,

ملخص المقالة :

این پژوهش با هدف بررسی صفات فنولوژیک، فیزیولوژیک و عملکرد دانه شش ژنوتیپ گلرنگ در شرایط تنش خشکی و بدون تنش در دو آزمایش مستقل، هر یک به صورت آزمایش‌های فاکتوریل در قالب طرح بلوک های کامل تصادفی با سه تکرار طی دو سال زراعی 95- 1394 و 96-1395 در ایستگاه تحقیقات کشاورزی سرابله اجرا شدند. یکی از آزمایش‌ها تحت شرایط بدون تنش آبیاری بر اساس نیاز گیاه و آزمایش دیگر به صورت تنش خشکی بدون هیچ گونه آبیاری و صرفاً با استفاده از نزولات آسمانی انجام گردید. میزان کل بارش در طی دوره رشد گیاه در سال‌های 95-1394 و 96-1395 به ترتیب 410/7 و 388/5 میلی‌متر بودند که با توزیع نامناسب در طی فصل رشد اتفاق افتادند. تیمارهای آزمایشی، فاکتور اول شامل شش ژنوتیپ گلرنگ و فاکتور دوم شامل تلقیح بذر با ازتوباکتر همراه با مصرف کود شیمیایی اوره در چهار سطح بدون مصرف هیچ منبع کودی (شاهد)، تلقیح بذر با ازتوباکتر، تلقیح بذر با ازتوباکتر+ 50 درصد نیتروژن از منبع اوره و 100 درصد نیتروژن از منبع کود شیمیایی اوره بر اساس نتایج آزمایش خاک، بودند. نتایج نشان داد اثر منبع نیتروژن و ژنوتیپ در هر دو شرایط تنش خشکی و بدون تنش بر روی کلیه صفات مورد بررسی در این آزمایش معنی‌دار بود. در شرایط تنش خشکی بیشترین عملکرد دانه متعلق به ژنوتیپ سینا در تیمارهای کاربرد 100 درصد کود شیمیایی نیتروژن و تیمار تلفیقی ازتوباکتر+ 50درصد مصرف کود شیمیایی نیتروژن (به ترتیب با میانگین 1408 و 1336 کیلوگرم در هکتار) برآورد شدند، که اختلاف معنی‌داری با یکدیگر نداشتند. در شرایط بدون تنش بیشترین عملکرد دانه به تیمار 100 درصد مصرف کود شیمیایی نیتروژن و ژنوتیپ PI-306974 با میانگین 2185 کیلوگرم در هکتار اختصاص داشت. در نتیجه، در شرایط بدون تنش ژنوتیپ PI-306974 با مصرف 100 درصد کود شیمیایی نیتروژن جهت حصول حداکثر عملکرد گلرنگ و تحت شرایط تنش خشکی کشت ژنوتیپ سینا با کاربرد 50 درصد کود شیمیایی نیتروژن به همراه ازتوباکتر می‌تواند در منطقه آزمایش به کار روند.

المصادر:

Akbari, P., A. Ghalavand, and S.A.M. Modarres Sanavy. 2010. Effects of different nutrition systems and biofertilizer (PGPR) on phenology period yield and yield components of sunflower (Helianthus annuus). Journal of Plant Production. 2(3): 119-134. (In Persian).

Akbari, S., M. Kafi, S. Rezvan, and S. Beidokhti. 2016. The effects of drought stress on yield, yield components and antioxidant of two garlic (Allium sativum ) ecotypes with different planting densities. Journal of Agroecology. 8(1): 95-106. (In Persian).

Ali, B. 2015. Bacterial auxin signaling comparative study of signaling: comparative study of growth induction in Arabidopsis thaliana and Triticum aestivum. Turkish Journal of Botany. 39: 1-9.

1995. AOAC (Association of Official Analytical Chemists). Official methods of analysis, 16th Edition. AOAC International, Gaithersburg, MD.

Arnon, A.N. 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal. 23: 112- 121.

Ashrafi, E., and K.H. Razmjoo. 2010. Effect of irrigation regimes on oil content and composition of safflower (Carthamus tinctorius) cultivars. Journal of the American Oil Chemists' Society. 87(5): 499-506.

Bagheri, H., B. Alibi, and M.R. Azimi Moghaddam. 2012. Effect of atrazine anti-transpiration application on improving physiological traits, yield and yield components of safflower under rainfed condition. Journal of Crops Improvement. 14 (2):1-16. (In Persian).

Ben Hamed, K., A. Castagna, E. Salem, A. Ranieri, and C. Abdelly. 2007. Sea fennel (Crithmum maritimum L.) under salinity conditions: a comparison of leaf and root antioxidant Plant Growth Regulation. 53: 185-194.

Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantites of protein utilizing the principle of protein-dye binding. Annals of Biochemistry. 72: 248-254.

Enayatgholizadeh, M.R., G. Fathi, and M. Razaz. 2011. Evaluation grain yield and yield component of three cultivars to drought stress different levels of nitrogen. Journal of Crop Ecophysiology. 5(1): 1-14. (In Persian).

Faraji, A. 2010. Seed yield in three species of brassica effect of rainfall and photothermal quotient in rainfed conditions of Gonbad. Seed and Plant Production Journal. 26(2): 109-121. (In Persian).

Fernanda, P., A.P. Bortolheiro, and O.A. Marcel-Silva. 2017. Physiological response and productivity of safflower lines under water deficit and rehydration. Annals of the Brazilian Academy of Sciences. 89(4): 3051-3066.

Filgueiras, L.M.B., and C.H.S.G. Meneses. 2015. Efeito das bactérias promotoras de crescimento de plantas na proteção contra o estresse hídrico. Journal of Biology and Pharmacy and Agricultural Management. 11: 21-30.

Flemmer, A.C., M.C. Franchini, and L.I. Lindström. 2015. Description of safflower (Carthamus tinctorius) phenological growth stages according to the extended BBCH scale. Annals of Applied Biology. 166: 331-339.

Franklin, A., G.F. Enéas, F. Claudivan, T.P. José, D. André, N. Azevedo, and C. Elton. 2010. Pretreatment with H₂O₂ in maize seeds: effects on germination and seedling acclimation to salt stress. Brazilian Journal of Plant Physiology. 22(2): 103-112.

Fuladvand, M., and A. Yadavi. 2015. Effect of plant density, rate and split application of nitrogen fertilizer on quality characteristics and nitrogen use efficiency of safflower under weed competition. Iranian Journal of Field Crops Research. 13(2): 358-368. (In Persian).

Ghorbanzadeh Neghab, M., G.H. Rassam, and A.R. Dadkhah. 2014. Evaluation of yield and seed fatty acid compositions of safflower (Carthamus tinctorius L.) cultivars in Shirvan region. Iranian Journal of Medicinal and Aromatic Plants. 30(3): 382-390. (In Persian).

Glick, B.R. 2014. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiological Research. 169: 30-39.

Goldani, M., and P. Rezvani. 2007. The effects of different irrigation regimes and planting dates on phenology and growth indices of three chickpea cultivars in Mashhad. Journal Agricultural Science Nature Resource. 14(1): 63-74. (In Persian).

Gururani, M.A., C.H. Upadhyaya, V. Baskar, J. Venkatesh, and A. Nookaraju. 2015. Plant growth-promoting rhizobacteria enhance abiotic stress tolerance in Solanum tuberosum through inducing changes in the expression of ROS-scavenging enzymes and improved photosynthetic performance. Journal of Plant Growth Regulation. 34(2): 245-258.

Hassanpour Lescokelaye, K., J. Ahmadi, J. Daneshyan, and S. Hatami. 2015. Changes in chlorophyll, protein and antioxidant enzymes on durum wheat under drought stress. Journal of Crop Breeding. 15: 76- 87. (In Persian).

Heshmati, S., A. Amini Dehaghi, and K. Fathi Amirkhiz. 2017. Effects of biological and chemical phosphorous fertilizer on grain yield, oil seed and fatty acids of spring safflower in water deficit conditions. Iranian Journal of Field Crop Science. 48: 159-169. (In Persian).

Jalilian, A., R. Ghobadi, and A. Farnia. 2010. Response of some photosynthesis system traits and leaf relative water content of corn [SC704] on different amounts of nitrogen fertilizer in different irrigation regimes. 5^th New Ideas in Agriculture Islamic Azad University of Isfahan. 14- 18. (In Persian).

Kim, Y.H., A.L. Khan, M. Waqas, and J.K. Shim. 2014. Silicon application to rice root zone influenced the phytohormonal and antioxidant responses under salinity stress. Plant Growth Regulation. 33: 137- 149.

Kutcher, H.R., S.S. Malhi, and K.S. Gill. 2005. Topography and management of nitrogen and fungicide affects diseases and productivity of canola. Agronomy Journal. 97(2): 533-541.

Lin, Y.L., Y.Y. Cho, W.D. Huang, and C.H. Kao. 2012. Effect of nitrogen deficiency on antioxidant status and Cd toxicity in rice seedlings. Plant Growth Regulation. 64 (3): 263-273.

Manivannan, P., C.A. Jaleel, B. Sankar, A. Kishorekumer, R. Somasundaram, G.M.A. Lakshmanan, and R. Panneerselvam. 2015. Growth, biochemical and surfaces, modification and proline metabolism in (Helianthus annus ) as induced by drought stress. Journal of Plant Nutrition. 59: 141-149.

Mirzaei, A., R. Naseri, S.M. Torab Miri, A. Soleymanifard, and A. Fathi. 2018. Response of yield and yield components of chickpea (Cicer arietinum ) cultivars to the application of plant growth promoting rhizohbacteria and nitrogen chemical fertilizer under rainfed conditions. Journal of Crop Ecophysiology. 44(4): 775-790. (In Persian).

Mirzakhani, M. 2017. Response of safflower (Carthamus tinctorius) agronomic characteristics to time, rate and methods of nitrogen applications. Journal of Crop Ecophysiology. 11(4): 875-888. (In Persian).

Moafi, R., and A.A. Teymiri. 2009. The effect of different amounts of N fertilizer in different sowing dates on phenological characterictics, grain yield and of canola (Hyola 401). Journal of Plant Ecophysiology. 1(3): 29-44. (In Persian).

Mohammadi, S. 2014. Evolution of grain yield and its components relationships in bread wheat genotypes under full irrigation and terminal water stress conditions using multivariate statistical analysis. Iranian Journal of Field Crops Research. 12(1): 99-109. (In Persian).

Mohammadi, S., H. Madani, and A. Rokhzadi. 2014. Effects of super absorbent application on yield and phonological traits of safflower cultivars in delay and spring sowing time at rain fed conditions in Sanandaj, Iran. New Finding in Agriculture. 9(1): 75-88. (In Persian).

Mojadam, M. 2017. Effect of drought stress on physiological characteristics and seed yield of sunflower at different levels of nitrogen. Electronic Journal Crop Production. 9: 121-136. (In Persian).

Movludi, A. 2015. Effect of nitrogen application on some characteristics of drought tolerance in spring barley. Journal of Crop Production. 8(3): 95-114.

Movludi, A., A. Ebadi, S. Jahanbakhsh, M. Davari, and G. Parmoon. 2013. Effects of water stress and nitrogen application on chlorophyll fluorescence and cell membrane stability in spring barley. The First National Conference on Planning and Environmental Protection, 21Feb. 2013. Islamic Azad University Hamedan (In Persian).

Namvar, A., and T. Khandan. 2015. Inoculation of rapeseed under different rates of inorganic nitrogen and sulfur fertilizer: Impact on water relations, cell membrane stability, chlorophyll content and yield. Archives of Agronomy and Soil Science. 61: 1137-1149.

Namvar, A., R. Seyed Sharifi, T. Khandan, and M. Jafari Moghadam. 2013. Organic and inorganic nitrogen fertilization effects on some physiological and agronomical traits of chickpea (Cicer arietinum ) in irrigated condition. Journal of Central European Agriculture. 14: 28–40.

Naseri, R., and A. Mirzaaei. 2010. Response of yield and yield components of safflower (Carthamus tinctorius ) to seed inoculation with azotobacter and azospirillum and different nitrogen levels under dry land conditions. American-Eurasian Journal Agriculture and Environment Science. 9(4): 445-449.

Niknam, V., N. Razavi, H. Ebrahimzadeh, and B. Sharifizadeh. 2006. Effect of NaCl on biomass proline and protein contents and antioxidant enzymes in seedling and calli of two Triginella species. Biologia Plantarum. 50: 591-596.

Omidi, A.H., H. Khazaei, P. Monneveux, and F. Stoddard. Effect of cultivar and water regime on yield and yield components in safflower (Carthamus tinctorius L.). Turkish Journal of Field Crops. 17: 10-15.

Ortiz, N., A. Armada, A. Duque, A. Roldan, and R. Azcon. 2015. Contribution of arbuscular mycorrhizal fungi and or bacteria to enhancing plant drought tolerance under natural soil conditions: Effectiveness of autochthonous or allochthonous strains. Journal of Plant Physiology. 174: 87–96.

Rahbarian, P., G. Afsharmanesh, and M.H. Shirzadi. 2010. Effects of drought stress and manure on relative water content and cell membrane stability in dragonhead (Dracocephalum moldavica). Plant Ecophysiology. 2: 9-13. (In Persian).

Segele, Z.T., and P.J. Lamb. 2005. Characterization and variability of Kiremt rainy season over Ethiopia. Meteorology of Atmospheric Physics. 89: 153–180.

Shahsavari, M.R. Determination of duration of developmental stages of sofea safflower cultivar by temperature and day length. Iranian Journal of Field Crops Research. 10(3): 555-562. (In Persian).

Shiyab, S.M., M.A. Shatnawi, R.A. Shibli, and M.W. Akash. 2013. Growth, nutrient acquisition and physiological responses of hydroponic grown tomato to sodium chloride salt induced stress. Journal Plant Nutrition. 36 (4): 665-676.

Soomro, M., H. Markhand, and B.A. Soomro. 2011. Screening Pakistani cotton for drought tolerance. Pakistan Journal of Botany. 44: 383-388.

Taleshi, K., A. Shokoh-Far, M. Rafiee, G. Noormahamadi, and T. Sakinejhad. 2012. Safflower yield respond to chemical and biotic fertilizer on water stress condition. World Applied Sciences Journal. 20: 1472-1477.

Tarighaleslami, M., M. Kafi, A. Nezami, and R. Zarghami. 2017. Examining interactions of chilling and draught stresses on chlorophyll (SPAD), RWC, electrolyte leakage and seed performance in three hybrid varieties of maize. Journal of Crop Breeding. 9(23): 131-156. (In Persian).

Turan, M., M. Gulluce, R. Çakmak, and F. Şahin. 2013. Effect of plant growth-promoting rhizobacteria strain on freezing injury and antioxidant enzyme activity of wheat and barley. Journal of Plant Nutrition. 36: 731-748.

Vardharajula, S., S.Z. Ali, M. Grover, G. Reddy, and V. Bandi. 2011. Drought-tolerant plant growth promoting Bacillus spp.: Effect on growth, osmolytes, and antioxidant status of maize under drought stress. Journal of Plant Interactions. 6: 1–14.

Walker, V., O. Couillerot, A. Von Felten, F. Bellvert, J. Jansa, M. Maurhofer, and G. Comte. 2011. Variation of secondary metabolite levels in maize seedling roots induced by inoculation with azospirillum, pseudomonas and glomus consortium under field conditions. Plant and Soil. 356: 151– 163.

Wange, W.B., Y.H. Kim, H. Lee, K.Y. Kim, X.P. Deng, and S.S. Kwak. 2009. Analysis of antioxidant enzymes activity during germination of alfalfa under salt and drought stresses. Plant Physiology Biochemstery. 47: 570- 577.

Yousefpoor, Z., A. Yadavi, H. Balouchi, and H. Farajee. 2014. Evaluation of some physiological, morphological and phonological characteristics in sunflower (Helianthus annuus L.) influenced by biological and chemical sources of nitrogen and phosphorus. Agroecology. 6(3): 508-519. (In Persian).