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  • تاثیر تنش شوری بر میزان فعالیت آنتی‌اکسیدان‌ها و رشد گیاهچه ژنوتیپ‌های گلرنگ

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کد مقاله : 535968 بازدید : 73 صفحه: 10 - 21

نوع مقاله: پژوهشی

تاثیر تنش شوری بر میزان فعالیت آنتی‌اکسیدان‌ها و رشد گیاهچه ژنوتیپ‌های گلرنگ

محورهای موضوعی : زراعت

تاریخ دریافت : 1393/06/20 تاریخ پذیرش : 1394/11/17 تاریخ انتشار : 1396/06/15

کلید واژه: آسکوربات پراکسیداز, گلرنگ, وزن خشک, سوپراکسیددیسموتاز,

چکیده مقاله :

به منظور ارزیابی اثر تنش شوری بر فعالیت آنزیم‎های آنتی اکسیدان و رشد گیاهچه ژنوتیپ های گلرنگ آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی در سه تکرار انجام شد. فاکتورهای آزمایشی شامل شش ژنوتیپ گلرنگ (اراک، اصفهان، خراسان، کوسه C111، AC-stirling و Saffire) و پنج سطح شوری شامل محلول 0 (شاهد)، 50 ، 100، 150 و 200 میلی مولار نمک طعام بود. نتایج آزمایش نشان داد که با افزایش شدت تنش شوری میزان فعالیت دو آنزیم آسکوربات پراکسیداز و سوپر اکسید‌دیسموتاز افزایش یافت اما در سطح شوری 200 میلی مولار نمک، فعالیت این آنزیم ها کاهش پیدا کرد. تحت تأثیر تنش شوری، بیشترین فعالیت آنزیم‎های آنتی‎اکسیدان سوپراکسید دیسموتاز و آسکوربات پراکسیداز در ژنوتیپ اصفهان و کمترین فعالیت این دو آنزیم در ژنوتیپ AC-Stirling مشاهده شد. برهمکنش تنش شوری و ژنوتیپ بر صفات گیاهچه‎ای گلرنگ معنی‎دار نبود اما ژنوتیپ‎های گلرنگ Saffire دارای بیشترین طول ساقه‎چه، وزن خشک ریشه‎چه و طول ریشه‎چه بر سایر ارقام برتری داشتند.

چکیده انگلیسی:

Salt stress is one of the major a biotic stresses especially in arid and semi-arid regions. This investigation was conducted to evaluate the effects of the salt stress on APX and SOD activities in safflower genotypes based in a factorial experiment as a randomized complete block design (CRD) with three replications. The experimental factors were six genotypes of safflower (Arak, Isfahan, Khorasan, C111, AC-stirling and Saffire) and five levels salt stress (0(control), 50, 100, 150 and 200Mm NaCl). There was a significant difference between genotypes in activity of SOD and APX enzymes under salt stress, with notable superiority of Isfahan genotype over other genotypes. Maximum activity of SOD was 99.16 uni/g Fw for Isfahan genotype under 150 Mm NaCl. In general, it seems that Isfahan genotype had more tolerance to salinity and AC-stirling was sensitive to salt stress. The results showed that Isfahan genotype had the greatest APX activity with 91unit/gFW while AC-stirling has the lowest content with 58unit/g FW at control treatment. The activity of both enzymes increased with salt stress, that this increase was dependent on genotype. APX and SOD activity decreased at the 200 Mm NaCl concentrations. The reason for declining in enzyme activity may be returned to enzyme protein damage by high salt stress or adaptation of plants to salt stress. Analysis of seedling traits showed that similar to antioxidants context, the genotypes of Isfahan and Ac-sterling had the most and the least values for all of the studied traits. The result showed that Isfahan genotype could be proposed for salt breeding programs in safflower.

منابع و مأخذ:


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