تاثیر نایسین A و بنزوات سدیم بر رفتار لیستریامونوسیتوژنز و برخی از پارامترهای میکروبی و شیمیایی در فیله ماهی فیتوفاگ (Hypophtalmichtysmolitrix) نگه‌داری شده در دمای 4 درجه سلسیوس
محورهای موضوعی :
علوم و صنایع غذایی
رضا صفری
1
(پژوهشکده اکولوژی دریای خزر، ساری، ایران.)
محمد رضا سعیدی اصل
2
(دانشگاه آزاد اسلامی، واحد سبزوار، گروه علوم و صنایع غذایی، سبزوار، ایران)
تاریخ دریافت : 1390/03/31
تاریخ پذیرش : 1390/09/25
تاریخ انتشار : 1390/09/01
کلید واژه:
ماهی فیتوفاگ,
نایسین A,
بنزوات سدیم,
لیستریامونوستیوژنز,
چکیده مقاله :
در این مطالعه تاثیر نایسین A و بنزوات سدیم بر تعداد لیستریامونوستیوژنز، برخی پارامترهای میکروبی (باکتری های مزوفیل، باکتری های سرماگرا و باکتری های لاکتیک) و پارامترهای شیمیایی (عدد پراکسید و TVN) در فیله ماهی فیتوفاگ نگه داری شده در دمای 4 درجه سلسیوس در زمان های صفر، 3، 6، 9 و 12 روز مورد ارزیابی قرار گرفت. به نسبت وزن اولیه فیله، مقدار تلقیح اولیه باکتری مشخص شده (4 واحد لگاریتمی) و پس از اسپری نمودن سوسپانسیون باکتریایی و جذب آن به سطح فیله، نمونه ها به مدت 15 دقیقه در محلول بنزوات سدیم 2% قرار داده شده و بعد از قرار گرفتن در دمای 4 درجه سلسیوس به مدت 10 دقیقه، محلول نایسین A با غلظت 15/0 گرم بر کیلوگرم ماهی بر روی نمونه ها اسپری گردید. نتایج نشان داد که در نمونه دارای ترکیب نایسین A و بنزوات سدیم تعداد لیستریا مونوسیتوژنز از 12/4 به 66/3 واحد لگاریتمی کاهش یافت. این در حالیست که در نمونه‌های فاقد ماده نگه دارنده تعداد لیستریا مونوسیتوژنز تلقیح شده از 43/4 به 14/5 واحد لگاریتمی افزایش داشته است. جمعیت باکتری های مزوفیل در نمونه‌های حاوی ماده نگه دارنده (بصورت ترکیبی) و نمونه‌های فاقد نگه دارنده به تریتب از 39/4 به 79/6 و از 48/4 به 93/7 واحد لگاریتمی افزایش داشته است. جمعیت باکتری های سرماگرا در نمونه‌های حاوی ماده نگه دارنده و نمونه‌های فاقد ماده نگه دارنده از 16/4 به 72/6 و از 34/4 به 92/7 واحد لگاریتمی و جمعیت باکتری های لاکتیک نیز در تیمارهای ذکر شده به ترتیب از 74/2 به 08/4 و 9/2 به 78/4 واحد لگاریتمی افزایش داشته است. افزایش مقدار عدد پراکسید و TVN در تیمار نایسین و بنزوات سدیم در مقایسه با نمونه های تیمار تفاوت نشان داد. نتیجه گیری کلی نشان می دهد که اگرچه مواد نگه دارنده مورد استفاده در این تحقیق به هنگام استفاده در محیط کشت آزمایشگاهی، جمعیت لیستریا مونوسیتوژنز را به صفر می رسانند ولی به هنگام تلقیح باکتری در بافت ماهی، پارامترهای مختلف بر اثرات ضدمیکروبی مواد نگه دارنده تأثیرگذار می باشند.
چکیده انگلیسی:
The effect of nisin A and sodium benzoate on Listeria monocytogenes, as well as some microbial (mesophilic, psychrotrophic and lactic acid bacteria) and chemical (peroxide and TVN) in silver carp (Hypophtalmichtys molitrix) fillet during storage period (0, 3, 6, 9 and 12 days) at 4˚C were evaluated. For this, Listeria monocytogenes (4 logCFU/g) was inoculated to the fillets and were dipped into 2% sodium benzoate solution for 15 min and left to stand for 10 min at 4˚C. Subsequently nisin A was added to the fillet (0/15 g/kg) and samples were kept at 4˚C while packaged in vacuum condition. The results showed that, application of nisin A and sodium benzoate decrease the number of Listeria monocytogenes from 4/12 to 3/66. However, in control groups the number of bacterium was increased from 4/43 to 5/14. Moreover, the number of mesophilic bacteria in treatment and control groups was increased from 4/39 to 6/79 and 4/48 to 7/93, respectively. The number of psychrotrophic bacteria in treatment and control groups was increased from 4/16 to 6/72 and 4/34 to 7/92, respectively. The similar result was achieved for lactic acid bacteria in which the number of these bacteria was increased from 2/74 to 4/08 and 2/9 to 4/78, respectively. Moreover, different peroxide value and TVN for treatment and control groups was achieved. In conclusion, application of nisin A and sodium benzoate showed different inhibitory effects on Listeria monocytogenes in culture media and silver carp.
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