Evaluation of Sulfonamide Antibiotic Residues of Honey Samples Produced in Different Regions of Qazvin Province by ELISA

Subject Areas : Journal of Chemical Health Risks

Ali Mehrabi ¹ , Razzagh Mahmoudi ² , Hajar khedmati Morasa ³ , Reza Norian ⁴ , Shaghayegh mosavi ⁵ , Zohreh Ahmadi ⁶ , Maryam Kazemi ⁷ , Ahad Alizadeh ⁸

1 - MSc. Food Hygiene and Safety, School of Public Health, Qazvin University of Medical sciences, Qazvin, Iran
2 - Professor, Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
3 - MSc. Food Hygiene and Safety, School of Public Health, Qazvin University of Medical sciences, Qazvin, Iran
4 - Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
5 - PhD Student in Molecular Medicine, Faculty of Medical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran
6 - MSc. Food Hygiene and Safety, School of Public Health, Qazvin University of Medical sciences, Qazvin, Iran
7 - MSc. Food Hygiene and Safety, School of Public Health, Qazvin University of Medical sciences, Qazvin, Iran
8 - Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran

Received: 2020-07-22 Accepted : 2021-05-26 Published : 2022-09-01

Keywords: Honey, Elisa, Antibiotic residues, Sulfonamide, Health hazard,

Abstract :

Honey is a unique food product containing bioactive compounds derived from bees and plantsNowadays, animal food products that may contain antibiotic residues have caused a lot of concern for the consumers. The presence of antibiotic residues in honey may be harmful to human health. One of the most important risks of antibiotic residues in food products is the occurrence of drug resistance in pathogenic bacteria in the body. Regarding the complications caused by these residues, the present research has investigated sulfonamide antibiotic residues in the honey samples consumed in Qazvin province. The present research is a cross-sectional study performed in different regions of Qazvin province in 2019. For this purpose, 80 honey samples were collected from different regions of Qazvin. The samples were transferred to the food safety laboratory of the Health Faculty under proper conditions. First, the samples were tested to check the sulfonamide antibiotic residue by the semi-quantitative ELISA assay. The data were recorded in SPSS 23, and data analysis was done by one-way analysis of variance (ANOVA) and (post hoc) Tukey test at the significant level of P˂0.05. According to the results of the ELISA assay, 10-120 ng of sulfonamide antibiotic residue was found in 23.75% (19 samples) of the samples. The findings showed that the highest and the lowest sulfonamide concentrations were respectively reported as 30.81 and 6.26 ppb, and the average sulfonamide residue was obtained as 14.50 ppb. According to the results, more than 75% of the honey samples collected from Qazvin province are free of sulfonamide or contain a little sulfonamide concentration. The research showed that most of the honey samples collected from different regions of Qazvin province are free of sulfonamide or contain a little sulfonamide concentration. Regarding the important role of honey consumption in health and the wide use of antibiotics in beehives, it is necessary to take the necessary actions to control the quality of this product.

References:

1. Kamkar A., Khodabakhshiyan S., 2017. Determination of the total phenolic, flavonoid and antioxidant activity of Sabalan honey. Journal of Veterinary Research. 72 (1), 53-61.

2. Küçük M., Kolaylı S., Karaoğlu Ş., Ulusoy E., Baltacı C., Candan F., 2007. Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chemistry. 100(2), 526-534.

3. Hasheminia S.M., Jamshidi M., Ostadi Y., 2019. Determination of Fluvalinate residue in honey samples of Damavand region. Food Science and Technology. 15(85), 459-466.

4. Arabsorkhi B., Sereshti H., 2018. Determination of tetracycline and cefotaxime residues in honey by micro-solid phase extraction based on electrospun nanofibers coupled with HPLC. Microchemical Journal. 140, 241-247.

5. Alvarez-Suarez J. M., Tulipani S., Romandini S., Bertoli E., Battino M., 2010. Contribution of honey in nutrition and human health: a review. Mediterranean Journal of Nutrition and Metabolism. 3(1), 15-23.

6. Mahmoudi R., Golchin A., Farhoodi A., 2014. A Review on Antibiotic Residues in Animal-derived Foods in Iran over the Last Thirty Years. Journal of Mazandaran University of Medical Sciences. 24(119), 213-222.

7. Bogdanov S., 2006. Contaminants of bee products. Apidologie. 37(1), 1-18.

8. Peres G.T., Rath S., Reyes F.G.R., 2010. A HPLC with fluorescence detection method for the determination of tetracyclines residues and evaluation of their stability in honey. Food Control. 21(5), 620-625.

9. Li J., Chen L., Wang X., Jin H., Ding L., Zhang K., Zhang H., 2008. Determination of tetracyclines residues in honey by on-line solid-phase extraction high-performance liquid chromatography. Talanta. 75(5), 1245-1252.

10. Bogdanov S., Haldimann M., Luginbühl W., Gallmann P., 2007. Minerals in honey: environmental, geographical and botanical aspects. Journal of Apicultural Research. 46(4), 269-275.

11. Ali F., Hossein N.V., Bahareh I., 2014. Survey on Tetracycline and Oxytetracycline antibiotic residues in

 

 

honeys produced in some honey hives in Khuzestan province using HPLC method in 2012. Iranian Veterinary Journal. 10(43), 65-73.

12. Dmitrienko S.G., Kochuk E.V., Apyari V.V., Tolmacheva V.V., Zolotov Y.A., 2014. Recent advances in sample preparation techniques and methods of sulfonamides detection–a review. Analytica Chimica Acta. 850, 6-25.

13. Wise R., 2002. Antimicrobial resistance: priorities for action. Journal of Antimicrobial Chemotherapy. 49(4), 585-586.

14. Samanidou V., Nisyriou S., 2008. Multi‐residue methods for confirmatory determination of antibiotics in milk. Journal of Separation Science. 31(11), 2068-2090.

15. Kaya S. E., Filazi A., 2010. Determination of antibiotic residues in milk samples. Kafkas Univ Vet Fak Derg, 16 (Suppl-A), S31-S35.

16. Jakšić S., Ratajac R., Prica N., Apić J., Ljubojević D., Stošić M.Ž., Baloš M.Ž., 2018. Methods of determination of antibiotic residues in honey. Journal of Analytical Chemistry. 73(4), 317-324.

17. Saridaki-Papakonstadinou M., Andredakis S., Burriel A., Tsachev I., 2006. Determination of tetracycline residues in Greek honey. Trakia Journal of Sciences. 4(1), 33-36.

18. Näumann G., Mahrt E., Himmelreich A., Mohring A., Frerichs H., 2012. Traces of contamination–well preserved in honey. Journal für Verbraucherschutz und Lebensmittelsicherheit. 7(1), 35-43.

19. Gunes M., Gunes N., Cibik R., 2009. Oxytetracycline and sulphonamide residues analysis of honey samples from Southern Marmara Region in Turkey. Bulgarian Journal of Agricultural Science. 15(2), 163-167.

20. Dluhošová S., Borkovcová I., Kaniová L., Vorlová L., 2018. Sulfonamide Residues: Honey Quality in the Czech Market. Journal of Food Quality. 2939207.

21. Gunes N., Cibik R., Gunes M.E., Aydin L., 2008. Erythromycin residue in honey from the Southern Marmara region of Turkey. Food Additives and Contaminants. 25(11), 1313-1317.

22. Bonvehí J.S., Gutierrez A.L., 2009. Residues of antibiotics and sulfonamides in honeys from Basque Country (NE Spain). Journal of the Science of Food and Agriculture. 89(1), 63-72.

23. Guillén I., Guardiola L., Almela L., Núñez-Delicado E., Gabaldón J.A., 2017. Simultaneous determination of nine sulphonamides by LC-MS for routine control of raw honey samples. Food Analytical Methods. 10(5), 1430-1441.

24. Korkmaz S.D., Kuplulu O., Cil G.I., Akyuz E., 2017. Detection of sulfonamide and tetracycline antibiotic residues in Turkish pine honey. International Journal of Food Properties. 20 (sup1), S50-S55.

25. Galarini R., Saluti G., Giusepponi D., Rossi R., Moretti S., 2015. Multiclass determination of 27 antibiotics in honey. Food Control. 48, 12-24.

26. Aarestrup F.M., Wegener H.C., Collignon P., 2008. Resistance in bacteria of the food chain: epidemiology and control strategies. Expert Review of Anti-infective Therapy. 6(5), 733-750.

27. Aalipour F., Mirlohi M., Jalali M., 2013. The study of the antibiotic consumption pattern in the production of animal originated food in Iran and its comparison to other countries. J Health Syst Res. 1572-1584.