• Home
  • Study of Potato (Solanum tuberosum L. Agria Cultivar) Microtuberization and Physiological Properties in Salinity Stress via Tissue Culture Technique

Share To

Article Url


Manuscript ID : JCHR-2302-1694 (R1) Visit : 583 Page: 747 - 758

10.22034/jchr.2023.1981240.1694

Article Type: Original Research

Study of Potato (Solanum tuberosum L. Agria Cultivar) Microtuberization and Physiological Properties in Salinity Stress via Tissue Culture Technique

Subject Areas : Journal of Chemical Health Risks

Nosrat Musavi 1 , Mostafa Ebadi 2 * , Mehdi Khorshidi 3 , Nahid Masoudian 4 , Hossein Hokmabadi 5

1 - Department of Plant Physiology, Damghan Branch, Islamic Azad University, Damghan, Iran
2 - Department of Plant Physiology, Damghan Branch, Islamic Azad University, Damghan, Iran
3 - Assistant Professor, Biology School, Damghan University, Damghan, Iran
4 - Department of Plant Physiology, Damghan Branch, Islamic Azad University, Damghan, Iran
5 - Associate Professor, Agricultural Education and Extension Institute, AREEO, Tehran, Iran

Received: 2023-02-26 Accepted : 2023-06-19 Published : 2025-09-28

Keywords: salinity stress, tissue culture, Physiological properties, Potato,

Abstract :

One of the most important crop plants is potato. Plant tissue culture is a very important technology in plant cultivation. In this investigation, the biochemical characterizes are investigated of potatoes cultivated by the tissue culture method. The lateral and apical buds were separated from the stem. They were sterilized and cultured in Murashige and Skoog culture medium. The concentrations of salt (0, 25, 50, 75 and 100 mM) have been used for salinity. After the growth of buds, the leaves were sampled and the chlorophylls, proline, sugars, protein, and antioxidant enzymes activity such as catalase, superoxide dismutase, guaiacol peroxidase, glutathione reductase and ascorbate peroxidase were evaluated. The results indicate that salt decreased protein and chlorophylls content. Sugars content and fresh weight have also been reduced with salinity, but the content of proline, malondialdehyde, and hydrogen peroxide were increased under salinity stress. Phenolic compounds, anthocyanin content, and antioxidant enzymes activity increased up to 50 mM salinity, but they decreased above this concentration. According to these results, it can be suggested that Agria cultivar potatoes are not recommended in salinity higher than 50 mM.

References:


  1. Food and Agriculture Organization. 2019 b. Food and Agricultural Organization of the United Nation, FAO Statistical Database. fromhttp: //www. fao. org/ faostat/en/#data. (Website).
    2.
  2. 2. Ahmed S., Zhou X., Pang Y., Xu Y., Tong C., Bao J.S., 2018. Genetic diversity of potato genotypes estimated by starch physicochemical properties and microsatellite markers. Food Chem. 257, 368–375.
    3.
  3. Calliope S.R., Lobo M.O., Sammán N.C., 2018. Biodiversity of Andean potatoes: Morphological, nutritional and functional characterization. Food Chem.238, 42–50.
    4.
  4. Sekmen A.H., Turkana L., Takiob S., 2007. Differential responses of antioxidative enzymes and lipid peroxidation to salt stress in salt-tolerant Plantago maritime and salt-sensitive Plantago media. Physiol Plant. 131, 399-411.
    5.
  5. Kaya M.D., Okc U.G., Atak M.C.¸ Ikili Y., Kolsarici O., 2006. Seed Treatments to Overcome Salt and Drought Stress During Germination in Sunflower (Helianthus annuus L.). European journal of agronomy. 24, 291–295.
    6.
  6. Hassani F., Moslemkhany C., Tahernezhad Z., 2021. Investigation of agronomic characteristics and yield stability of some medium-late maturing potato genotypes. Iranian Journal of Seed Science and Research. 8(3), 311-323.
    7.
  7. Hannachi C., Debergh P., Zid E., Messai A., Mehouachi T., 2004. Tubérisation sous stress saline de vitroplants de pomme de terre (Solanum tuberosum L.). Biotechnol Agron Soc Environ. 8(1), 9-13.
    8.
  8. Sudhersan S., Manuel J., Ashkanani J., Al-Ajeel A., 2012. In vitro screening of potato cultivars for salinity tolerance. American-Eurasian Journal of Sustainable Agriculture. 6(4), 344–348.
    9.
  9. Zaman M.S., Ali G.M., Muhammad A., 2015. In vitro screening of salt tolerance in potato (Solanum tuberosum L.) varieties. Sarhad Journal of Agriculture. 31(2), 106–113.
    10.
  10. Munira S., Hossain M.M., Zakaria M., 2015. Evaluation of potato varieties against salinity stress in Bangladesh. International Journal of Plant and Soil Science. 6(2), 73–81.
    11.
  11. Murshed R., Najla S., Albiski F., Kassem I., Jbour M., Al-Said H., 2015. Using growth parameters for in-vitro screening of potato varieties tolerant to salt stress. Journal of Agricultural Science and Technology. 17(2), 483–494.
    12.
  12. Roy S.J., Negrao S., Tester M., 2014. Salt resistant crop plants. Curr Opin Biotechnol.26, 115–124.
    13.
  13. Jarsma R., Boer A.H., 2018. Salinity Tolerance of Two Potato Cultivars (Solanum tuberosum L.) Correlates with Differences in Vacuolar Transport Activity. Front Plant Sci. 1-12.
    14.
  14. Toma R.S., 2022. Minitubers production of four potato (solanum tuberosum L.) Cultivars by tissue culture technique. Iraqi Journal of Agricultural Sciences. 53(5), 1058- 1066.
    15.
  15. Hassanpanah D., 2022. Introduction of suitable nutrient solution for mini tuber production of vegetable cultivars. Applied Potato Science. 5(2), 1-6.
    16.
  16. Lichtenthaler H.K., 1987. Chlorophyll and carotenoids: pigments of photosynthetic bio membranes. Method Enzym. 148, 350-382.
    17.
  17. Gao W.J., 2000. The exprimental technology of plant physiology. Word Book Press, Xian. 89- 258.
    18.
  18. Alexieva V., Sergiev I., Mapelli S., Karonov E., 2001. The effect of drought and ultraviolet radiation on growth and stress markeer in pea and wheat. Plant Cell Environ. 24, 1337- 1344.
    19.
  19. Wagner G.J., 1979. ­Content and vacuol/­extra vacuole distribution of neutral sugar, free amino acid and anthocyanins in protoplast. Plant Physiology. 64, 88- 93.
    20.
  20. Bates L.S., Waldern R.P., Tare I.D., 1973. Rapid determination free proline for water stress studies. Plant Soil. 29, 205- 207.
    21.
  21. Roberts E.J., Martin L.F., 1959. Progress in determining organic non-sugars of sugarcane juice that affect sugar refining, Bone Char Research Project, Inc. 67-99.
    22.
  22. Heath R.L., Packer L., 1968. Photo peroxidation in isolated chloroplast. kinetics and stoichiometry of fatty acid peroxidation. Archive of Biochemistry and Biophysics. 125, 189-198.
    23.
  23. Bradford M.M., 1979. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Bichem. 72, 248-254.
    24.
  24. Dhindsa R.S., Plump-dhindsa P., Thorpe T.A., 1981. Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase. J Exp Bot. 32, 93-101.
    25.
  25. Maehly A.C., Chance B., 1959. The assay of catalase and peroxidase. Methods of Biological Analysis. 1, 357-425.
    26.
  26. Chen G.X., Asada K., 1989. Ascorbate peroxidase in tea leaves - occurrence of 2 isozymes and the differences in their enzymatic and molecular properties. Plant and Cell Physiology. 30, 987-998.
    27.
  27. Upadhyaya A., Snkhla D., Davis T.D., Sankhla N., Smith B.N., 1985. Effected of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves. J Plant Physiol. 121, 453-461.
    28.
  28. Klapheck S., Zimmer I., Cosse H., 1990. Scavenging of hydrogen peroxidein the indosperm of Ricinus communis by ascorbate peroxidase. Plant Cell Physiol. 31, 1005-1013.
    29.
  29. Bajguz A., Hayat S., 2008. Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiol. 10, 1016-1020.
    30.
  30. Hussein M.M., Balbaa L.K., Gaballah M.S., 2007. Salicylic Acid and Salinity Effects on Growth of Maize plants. J of Agri and Biol Sci. 3(4), 321-328.
    31.
  31. Pioter Grazyana K., 2005. Antioxidant defence in leaves of C3- C4 plant under salinity stress. Physiology Plantarum. 125, 31- 40.
    32.
  32. David W., Bollivar S., Beale I., 1996. The chlorophyll Biosynthetic Enzyme Mg-protoporphyrin IX Monomethyl Ester (Oxidative Cyclase). Plant Physio. l (112), 105-114.
    33.
  33. Fadaladeen L.H., Toma R.S., Saheen A.A., Ahmed H.B., 2022. A Rapid Micropropagation Protocol for Sweet Potato (Ipomoea batatas L.) Via Tissue Culture Technique. Diyala Agricultural Sciences Journal. 14(1), 31-39.
    34.
  34. Masoudi-Sadaghiani F., Abdollahi Mandoulakani B., Zardoshti M.R., Rasouli-Sadaghiani M.H., Tavakoli A., 2011. Response of proline, soluble sugars, photosynthetic pigments and antioxidant enzymes in potato (Solanum tuberosum L.) to different irrigation regimes in greenhouse condition. Australian journal of crop sciences. 5(1), 55-60.
    35.
  35. Rojas-Paadilla C.R., Vasquez-Villalobos V.J., Vital C.E., Rojas J.C., Rios N.H., Ninaquispe V.P., Espinoza M.S., 2019. Phenolic compounds in native potato (Solanum tuberosumL.) cooking water, with potential antioxidant activity. Food Sci Technol Campinas. 39(1), 66-71.
    36.
  36. Kim J., Soh S.Y., Bae H., Nam S.Y., 2019. Antioxidant and phenolic contents in potatoes (Solanum tuberosumL.) and micropropagated potatoes. Appl Biol Chem.62, 17-25.
    37.
  37. Lee S.H., Oh S.H., Hwang I.G., Kim H.Y., Woo K.S., Woo S.H., Kim S.H., Lee J., Jeong H.S., 2016. Antioxidant Contents and Antioxidant Activities of White and Colored Potatoes (Solanum tuberosum L.). Prev Nutr Food Sci. 21(2), 110–116.
    38.
  38. Mamiya K., Tanabe K., Onishi N., 2020. Production of potato (Solanum tuberosum L.) microtubers using plastic culture bags. Plant Biotechnology. 37, 233–238.
    39.
  39. Sreenivasulu N., Sopory S.K., Kavi Kishor P.B., 2007. Deciphering the regulatory mechanisms of abiotic stress tolerance in plants by genomic approaches. Gene. 388, 1-13.
    40.
  40. Azevedo-Neto A.D., Ptisco J.T., Eneas-Filho J., Abreu C.F.B., Gomez-Filho E., 2006. Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environ Exp Bot. 56, 87-94.
    41.
  41. Maas E.V., Hoffman G.J., 1977. Crop salt tolerance-current assessment. J Irrig Drainage. 103, 115-134.
    42.
  42. Tränkner M.; Tavakol E.; Jákli B., 2018. Functioning of potassium and magnesium in photosynthesis, photosynthate translocation and photoprotection. Physiol Plant. 163, 414–431.
    43.
  43. Fadladeen, L.H., Toma, R.S., 2020. Embryo Culture and in Vitro Clonal Propagation of Oak (Quercus aegilops L.). The Iraqi Journal of Agricultural Science. 51(1), 347-355.
    44.
  44. Hassani F., Moslemkhani K., Kheiri F., Hosseininejadian J., Mobasser S., Rezvani E., Gharakhani A., 2018. Testing Value for Cultivation and Use(VCU) of foreign Potato cultivars. Seed and Plant Certification and Registration Institute (Research Report).
    45.
  45. Munthali C., Kinoshita R., Onishi K., Rakotondrafara A., Mikami K., Koike M., Tani M., Palta J., Aiuchi D., 2022. A Model Nutrition Control System in Potato Tissue Culture and Its Influence on Plant Elemental Composition. Plants. 11, 2718.
    46.
  46. Rahnama H., Ebrahimzadeh H., 2006. Antioxidant Isozymes Activities in Potato Plants (Solanum tuberosum L.) Under Salt Stress. Journal of Sciences. 17(3), 225-230.
    47.
  47. Aghaei K., Ehsanpour A.A., Komatsu S., 2009. Potato responds to salt stress by increased activity of antioxidant enzymes. Journal of Integrative Plant Biology. 51(12), 1095-1103.
    48.

Related articles

The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2025

Home| Login| About Us| Contact Us|
[فارسی] [العربية] [fa] [ar]