Analysis of Soil Populations of Aspergillus flavus Link. from Pistachio Orchards in Iran for Vegetative Compatibility

Subject Areas : Microbiology

M. Houshyar Fard ¹ , H. Rouhani ² , M. Falahati Rastegar ³ , E. Mehdikhani Moghaddam ⁴ , S. Malekzadeh Shafaroudi ⁵ , C. Probst ⁶

1 - Department of Plant Protection, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2 - Department of Plant Protection, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 - Department of Plant Protection, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
4 - Department of Plant Protection, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
5 - Department of Agricultural Biotechnology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
6 - University of Arizona, School of Plant Sciences, Tucson 85721

Received: 2015-10-08 Accepted : 2015-10-08 Published : 2014-09-01

Keywords: Pistachio, Rafsanjan, Aspergillus flavus, Damghan, Vegetative compatibility,

Abstract :

One hundred-thirty soil isolates of A. flavus were collected from commercial pistachio orchards in two main production regions including Rafsanjan (Kerman Province, south-eastern of Iran) and Damghan (Semnan Province, central north of Iran) and assayed for vegetative compatibility groups (VCGs). The sixteen and twenty VCGs were identified for 41 and 37 nit-mutant producing isolates of A. flavus. The variability in morphology was found among A. flavus isolates from different VCGs, but no variability among isolates from the same VCG. VCG diversity for A. flavus isolates from Damghan and Rafsanjan was 54 and 39%, respectively. The high VCG members in small size-isolates of A. flavus from Damghan indicated high genetic variability in fungal population. Because of the small number of sclerotium-producing isolates of A. flavus, we did not determine the relationships between sclerotium production with VCG in two pistachio production regions. This study was the first to determine the strain and VCG diversity of soil isolates of A. flavus from pistachio orchards in Iran