مطالعه بیوانفورماتیکی توالی‌های پروتئین آلیناز در گیاهان
محورهای موضوعی : اکوفیزیولوژی و فیتوشیمی گیاهان دارویی و معطر
کلید واژه: آلیسین, آلیناز, بیوانفورماتیک, فیلوژنی, همردیفی,
چکیده مقاله :
آلیناز (EC 4.4.1.4) آنزیمی وابسته به پیریدوکسال فسفات است که تبدیل آلین به آلیسین را انجام می‌دهد. آلیسین ترکیبی ارگانوسولفوره با ویژگی‌های ضد‌میکروبی، ضد‌سرطانی، ضد‌دیابتی و آنتی‌اکسیدانی است. مطالعه بر روی آلیناز و درک چگونگی تنظیم تولید آلیسین برای تولید در محل آن جهت مصارف کلنیکی ضروری است. در این مطالعه، توالی‌های پروتئین آلیناز گیاهی از سایت ExPASy و NCBI دریافت‌شدند و با ClustalW همردیفی صورت‌پذیرفت. نتایج این همردیفی شباهت 39-98 درصدی را میان توالی‌ها نشان‌داد. درخت فیلوژنی برای این توالی‌های پروتئینی با استفاده از نرم‌افزار MEGA4 و با روش نزدیکترین همسایه رسم‌شد. خانواده‌های Alliacea و Poaceae به خوبی در این درخت از هم تفکیک شده‌بودند. همچنین به کمک ابزارCD search موجود در سایت NCBI، EFG alliinase و AAT به عنوان دو دومین این پروتئین شناخته‌شدند.
Alliinase (EC 4.4.1.4) is a pyridoxal phosphate dependent enzyme which induces the conversion of alliin into allicin. Allicin is an organosulfur compound which has antimicrobial, anticancer, antidiabetic and antioxidant properties. A detailed study on the alliinase and regulation of allicin production are critical for on-site production of allicin for clinical usage. In this study, protein sequences of alliinase were obtained by ExPASy and NCBI and were aligned by using ClustalW. The sequence similarities range from approximately 39 -98%. A phylogenetic tree was built for the 10 alliinase protein sequences using MEGA4 by the Neighbor-Joining Method. Alliaceae and Poaceae families were separated carefully on the tree. Furthermore, EGF alliinase and AAT are two domain of allinase which were found by CD search of NCBI
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