Bioinformatics study of alliinase protein sequences in plants

Subject Areas : Ecophysiology & Phytochemistry of Medicinal and Aromatic Plants

Received: 2016-04-19 Accepted : 2016-04-19 Published : 2016-02-20

Keywords: phylogeny, alliinase, allicin, Bioinformatics, Alignment,

Abstract :

Alliinase (EC 4.4.1.4) is a pyridoxal phosphate dependent enzyme which induces the conversion of alliin into allicin. Allicin is an organosulfur compound which has antimicrobial, anticancer, antidiabetic and antioxidant properties. A detailed study on the alliinase and regulation of allicin production are critical for on-site production of allicin for clinical usage. In this study, protein sequences of alliinase were obtained by ExPASy and NCBI and were aligned by using ClustalW. The sequence similarities range from approximately 39 -98%. A phylogenetic tree was built for the 10 alliinase protein sequences using MEGA4 by the Neighbor-Joining Method. Alliaceae and Poaceae families were separated carefully on the tree. Furthermore, EGF alliinase and AAT are two domain of allinase which were found by CD search of NCBI

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