plant growth regulators

دسترسی آزاد مقاله

1 - اثر مصرف خارجی اسید آبسزیک و سیتوکینین بر گندم (Triticum aestivum)
سیف الله کیا چهارباغي

اسید آبسزیک (ABA) و سیتوکینین CK)) علاوه بر تنظیم فرآیند های مرتبط با پیری در شکل گیری قدرت منبع و مخزن نقش اساسی دارند. این تحقیق به منظور بررسی اثرات مصرف خارجی این دو تنظیم کننده رشد با غلظت 100 میکرو مولار در 9 سطح : 1 - شاهد- 2- Ck در زمان ظهور سنبله 3- Ck در 14 رو چکیده کامل

اسید آبسزیک (ABA) و سیتوکینین CK)) علاوه بر تنظیم فرآیند های مرتبط با پیری در شکل گیری قدرت منبع و مخزن نقش اساسی دارند. این تحقیق به منظور بررسی اثرات مصرف خارجی این دو تنظیم کننده رشد با غلظت 100 میکرو مولار در 9 سطح : 1 - شاهد- 2- Ck در زمان ظهور سنبله 3- Ck در 14 روز بعد از ظهور سنبله 4- ABA در زمان ظهور سنبله 5 - ABA در 14 روز بعد از ظهور سنبله - Ck در زمان ظهور سنبله و ABA در 14 روز بعد از ظهور سنبله 7- ABA در زمان ظهور سنبله و Ck در 14 روز بعد از ظهور سنبله 8- ABA در هر دو زمان 9 - Ckدر هر دو زمان، بر عملکرد، اجزای عملکرد گندم در مراحل رشد دانه در سال زراعی 88-1387 در مزرعه تحقیقاتی دانشگاه آزاد اسلامی واحد کرج در قالب طرح بلوک های کامل تصادفی در 3 تکرار اجرا گردید. نتایج حاصل از مصرف خارجی این دو هورمون در مراحل مختلف رشد زایشی( ظهور سنبله و14 روز بعد از ظهور سنبله) نشان داد که عملکرد دانه، شاخص برداشت و وزن هزار دانه به طور معنی داری تحت تاثیر مصرف خارجی این دو هورمون گرفتند. بیشترین عملکرد دانه به میزان 09/738 (گرم در متر مربع) مربوط به مصرف خارجی سیتوکینین در زمان ظهور سنبله بود. بیشترین شاخص برداشت و تعداد دانه در خوشه با مصرف خارجی سیتوکینین در هر دو زمان به دست آمد. بالاترین وزن هزار دانه مربوط به مصرف خارجی سیتوکینین در زمان ظهور سنبله و اسید آبسزیک در 14 روز بعد از ظهور سنبله مربوط می شود. نتایج حاصل از این تحقیق نشان داد که سیتوکینین از طریق تاثیر بر تقسیم سلولی و اسید آبسزیک از طریق تاثیر بر سرعت پر شدن دانه به طور معنی داری عملکرد دانه را افزایش می دهند. پرونده مقاله

دسترسی آزاد مقاله

2 - تأثیر پرایمینگ بذر با سالیسلیک اسید و محلول‌پاشی برگی براسینواستروئید بر عملکرد و برخی از صفات فیزیولوژیکی رازیانه (Foeniculum vulgare Mill) در شرایط کم‌آبیاری
طاهره مجردی محمدرضا یاورزاده فاطمه شیرزادی

10.30495/jcep.2020.679369

استفاده از تنظیم‌کننده‌های رشد یکی از راه‌های کاهش اثرات نامطلوب تنش خشکی است. به منظور بررسی تأثیر سطوح مختلف تنش کم‌آبیاری، پرایمینگ بذری با سالیسیلیک اسید و محلول‌پاشی برگی براسینواستروئید بر صفات فیزیولوژیکی و عملکرد رازیانه آزمایشی در سال زراعی 1397 در ایستگاه تحقی چکیده کامل

استفاده از تنظیم‌کننده‌های رشد یکی از راه‌های کاهش اثرات نامطلوب تنش خشکی است. به منظور بررسی تأثیر سطوح مختلف تنش کم‌آبیاری، پرایمینگ بذری با سالیسیلیک اسید و محلول‌پاشی برگی براسینواستروئید بر صفات فیزیولوژیکی و عملکرد رازیانه آزمایشی در سال زراعی 1397 در ایستگاه تحقیقاتی دانشگاه آزاد اسلامی واحد بم واقع در استان کرمان آزمایشی به صورت اسپلیت- فاکتوریل در قالب طرح پایه بلوک‌های کامل تصادفی با سه تکرار اجرا شد. کرت‌های اصلی شامل سه سطح آبیاری شامل 50 (به عنوان شاهد)، 75 و 100 میلی‌متر تبخیر از سطح تشتک تبخیر کلاس A بود. فاکتور فرعی شامل پرایمینگ بذر با سالیسیلیک اسید (در دو سطح عدم کاربرد و پرایمینگ بذر با غلظت 1600 میکرو‌مولار) و محلول‌پاشی برگی براسینواستروئید در سه سطح با غلظت‌های صفر ، 8-10 و 7-10مولار در مراحل شش‌برگی و ظهور چتر بود. صفات مورد مطالعه شامل محتوای نسبی آب برگ، محتوای کلروفیل a، b و کل، محتوای کاروتنوئید، آنتوسیانین، قندهای محلول، درصد اسانس و عملکرد دانه بود. طبق نتایج حاصل کم‌آبیاری سبب کاهش محتوای آب نسبی برگ، محتوای کلروفیل، کاروتنوئید و عملکرد دانه و افزایش آنتوسیانین، قندهای محلول و درصد اسانس شد. پرایمینگ با سالیسیلیک اسید و براسینواستروئید تأثیر مثبتی بر اغلب صفات مورد مطالعه داشت. بیشترین میزان کلروفیل کل (54/1) و عملکرد دانه (1281 کیلوگرم در هکتار) در تیمار 50 میلی‌متر و پرایمینگ با سالیسیلیک اسید مشاهده شد. غلظت‌های 7-10 و 8-10 مولار براسینواستروئید به ترتیب سبب افزایش 4/5 و 2/3 درصـدی محتوای آب نسبی برگ شد. پرایمینگ با سالیسیلیک اسید در غلظت‌های صفر، 8-10 و 7-10 مولار براسینواستروئید درصد اسانس را به ترتیب 8، 4/24 و 6/13 درصد افزایش داد. در تیمارهای کم‌آبیاری 50، 75 و 100 میلی‌متر به ترتیب 5/4، 7/6 و 14 درصد افزایش قندهای محلول در اثر پرایمینگ با سالیسیلیک اسید مشاهده شد. با توجه به نتایج، پرایمینگ سالیسیلیک اسید و محلول‌پاشی براسینواستروئید می تواند تا حدودی اثرات منفی ناشی از کم‌آبیاری را کاهش دهد. پرونده مقاله

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3 - اثر کاربرد توام باکتری‌های محرک رشد و سطوح ورمی‌کمپوست بر عملکرد کمّی و کیفی کلزای پاییزه (.Brassica napus L)
رضا منعم علیرضا پازکی علی عبدزادگوهری

مدیریت تلفیقی تغذیه گیاه، راهکاری مناسب برای افزایش بهره‌وری گیاهان زراعی و بهبود محیط زیست به شمار می رود. به منظور بررسی اثر کاربرد باکتری‌های محرک رشد همراه با سطوح مختلف ورمی‌کمپوست بر عملکرد کمّی و کیفی کلزا، آزمایشی در سال&lrm; &lrm;زراعی 94-1393 به صورت مزرعه چکیده کامل

مدیریت تلفیقی تغذیه گیاه، راهکاری مناسب برای افزایش بهره‌وری گیاهان زراعی و بهبود محیط زیست به شمار می رود. به منظور بررسی اثر کاربرد باکتری‌های محرک رشد همراه با سطوح مختلف ورمی‌کمپوست بر عملکرد کمّی و کیفی کلزا، آزمایشی در سال&lrm; &lrm;زراعی 94-1393 به صورت مزرعه‌ای به اجرا درآمد. تیمارهای آزمایش شامل تلقیح و عدم تلقیح (شاهد) بذرهای کلزا با باکتری‌های محرک رشد و کاربرد سطوح مختلف ورمی‌کمپوست به مقدار صفر (شاهد)، 4، 8 و 12 تن در هکتار بودند. نتایج نشان داد که اثرات ساده و برهم‌کنش کاربرد ورمی‌کمپوست و باکتری‌های محرک رشد بر صفات کمّی و کیفی کلزا معنی‌دار بودند. به طوری که با افزایش مقدار کاربرد ورمی‌کمپوست از ۴ تا ۱۲ تن در هکتار، افزایش معنی‌داری در صفات مورد بررسی نسبت به شاهد مشاهده شد. هم چنین، تلقیح بذرها با باکتری‌های محرک رشد نیز باعث افزایش مقدار صفات اندازه‌گیری شده نسبت به شاهد گردید. کاربرد 12 تن ورمی‌کمپوست در هکتار به همراه باکتری‌های محرک رشد ، باعث افزایش تعداد خورجین در بوته، وزن هزار دانه، عملکرد بیولوژیک، عملکرد دانه، شاخص برداشت و عملکرد روغن به ترتیب به میزان 28.63 عدد، 4.68 گرم، 2.10785 کیلوگرم، 3484.2 کیلوگرم، 38.02% و 1795.93 کیلوگرم در هکتار نسبت به شاهد شد. کاربرد 12 تن ورمی‌کمپوست در هکتار به همراه باکتری‌های محرک رشد در سه صفت عملکرد دانه، شاخص برداشت و عملکرد روغن منجر به افزایش به ترتیب 83، 45 و 43 درصد نسبت به شاهد گردید. بنابراین، در خاک‌هایی با کمبود مواد آلی، کاربرد هم‌زمان ورمی‌کمپوست و باکتری‌های محرک رشد می‌تواند سبب افزایش معنی‌داری در عملکرد کمی و کیفی کلزا شود. پرونده مقاله

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4 - تأثیر سطوح مختلف اسید ایندول-3-بوتیریک و اسید نفتالن استیک بر ریشه‌زایی قلمه‌ شاخه‌ی زیتون رقم ماری (Olea europaea cv. Mari)
بهزاد کاویانی محمد رضا صفری مطلق اصغر حسنخواه علیرضا اسلامی

10.30495/iper.2022.1929388.1700

زیتون (Olea europaea) گونه ای درختی است که دانه رست آن رشد کندی دارد و تکثیر از طریق بذر، زمان بر است و احتمال تغییرات ژنتیکی را افزایش می دهد. مناسب ترین روش تکثیر زیتون، استفاده از قلمه ی شاخه می باشد؛ با این وجود قلمه ی نیمه خشبی زیتون، سخت ریشه زا است. هدف از پژوهش چکیده کامل

زیتون (Olea europaea) گونه ای درختی است که دانه رست آن رشد کندی دارد و تکثیر از طریق بذر، زمان بر است و احتمال تغییرات ژنتیکی را افزایش می دهد. مناسب ترین روش تکثیر زیتون، استفاده از قلمه ی شاخه می باشد؛ با این وجود قلمه ی نیمه خشبی زیتون، سخت ریشه زا است. هدف از پژوهش حاضر، استفاده از غلظت های 2، 3 و 4 گرم بر لیتر اسید ایندول-3-بوتیریک (IBA) و اسید نفتالن استیک (NAA)، به صورت انفرادی و در ترکیب با یکدیگر، برای تسهیل ریشه‌زایی قلمه ی شاخه ی زیتون رقم ماری بود. بستر کاشت مورد استفاده، ماسه بود. این پژوهش به صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با سه تکرار انجام شد. درصد ریشه‌زایی، تعداد ریشه، طول ریشه، وزن تر و وزن خشک ریشه اندازه گیری شد. مطابق با نتایج این آزمایش، بالاترین درصد ریشه‌زایی (30/83 درصد)، بیشترین تعداد ریشه (09/17)، بالاترین طول ریشه (67/22 سانتی متر) و بیشترین وزن خشک (30/2 گرم) ریشه در قلمه های تیمارشده با 3 ‌گرم بر لیتر NAA همراه با 3 ‌گرم بر لیتر IBA به‌دست آمد. بیشترین وزن تر (83/4 گرم) ریشه در قلمه‌های تیمارشده با 4 ‌گرم بر لیتر NAA همراه با 3 ‌گرم بر لیتر IBA حاصل شد. پایین ترین درصد ریشه زایی، کمترین تعداد ریشه، پایین ترین طول ریشه و کمترین وزن تر و خشک ریشه مربوط به قلمه های شاهد بود. استفاده از تیمار حاوی 3 ‌گرم بر لیتر NAA همراه با 3 ‌گرم بر لیتر IBA برای ریشه‌زایی بهینه‌ی قلمه‌ی ساقه‌ی زیتون توصیه می‌شود. پرونده مقاله

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5 - Effect of explants and growth regulators on direct organogenesis of Dracocephalum kotschyi Boiss. via tissue culture technique
محمود اطرشی کوثر مرادی

Background & Aim: Dracocephalum genus belongs to the Lamiaceae family which has eight species in Iran. Dracocephalum kotschyi Boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. In addition, this species is used in Iranian traditi چکیده کامل

Background & Aim: Dracocephalum genus belongs to the Lamiaceae family which has eight species in Iran. Dracocephalum kotschyi Boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. In addition, this species is used in Iranian traditional medicine. The aim of current study was to examine the various growth regulations and explants on direct organogenesis of Dracocephalum kotschyi using tissue culture technique. Experimental: Various concentrations of BAP, IBA and NAA belong to auxin and cytokinin, as two important plant growth regulators (PGR), with shoot tip explants; hypocotyl and cotyledon were investigated in plant micropropagation of Dracocephalum kotschyi. Results & Discussion: Results indicated that the best treatment and explants on regeneration Dracocephalum kotschyi was MS medium containing 5 mg/L BAP, and 0.2 mg/L NAA on shoot tip explants. Regenerated explants on MS medium containing 1 mg/L BAP and 0.5 mg/L IBA had elongation growth and the elongation growth medium, plantlets were rooted. Rooted plantlets were then transferred to the hardening. After consistency of the regeneration plant seedlings Dracocephalum kotschyi, about 95% of seedling transferred to greenhouse suitable growing. Industrial and practical recommendations: According to the results of this research, we can start to proliferate and produce the effective matter of medicinal plant, such as Dracocephalum kotschyi. The most important benefit of this method is to prevent from extinction and to protect the species out of the environment. پرونده مقاله

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6 - In vitro propagation of orchid Phalaenopsis amabilis (L.) Blume var. Jawa
Mozhdeh Asa Behzad Kaviani

10.30495/ijpp.2020.672571

A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fort چکیده کامل

A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fortified with various concentrations of kinetin (KIN) and indole-3-butyric acid (IBA), either individually or in combination. A combination of 1.00 mg l-1 KIN + 1.00 mg l-1 IBA was found to be suitable for regeneration of most measured characteristics especially maximum PLBs regeneration (30.40/plantlet), leaf number (5.93/plantlet), and root number (8.36/plantlet) from protocorm explants. The maximum number of plantlets (11.66) was calculated on MS medium supplemented with 1.00 mg l-1 KIN + 0.50 mg l-1 IBA, followed by 1.00 mg l-1 KIN + 1.00 mg l-1 IBA (10.33). Plantlets were transplanted to pots filled with cocochips and sphagnum moss (70:30) for acclimatization and transferred to the greenhouse. Upon ex vitro transfer, 90% of plants survived. پرونده مقاله

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7 - Alleviation of the effects of on drought stress Verbascum nudicuale by methyl jasmonate and titanium dioxide nanoparticles
Fatemeh Ghasemlou Hamzeh Amiri Roya Karamian Asghar Mirzaie-asl

10.30495/ijpp.2019.668857

Drought stress causes severe metabolic dysfunctions by formation of oxidative stress that may lead to damage in DNA, inactivation of enzymes, and lipid peroxidation. Plants have developed different morphological, physiological, and biochemical mechanisms to withstand th چکیده کامل

Drought stress causes severe metabolic dysfunctions by formation of oxidative stress that may lead to damage in DNA, inactivation of enzymes, and lipid peroxidation. Plants have developed different morphological, physiological, and biochemical mechanisms to withstand the drought stress. Plant growth regulators (PGRs) are one of the most important endogenous substances involved in the amelioration of tolerance in various plant species. In this study, the possible recovery ability of Verbascum nudicuale plants from drought stress conditions was assessed using methyl jasmonate (MJA), titanium dioxide nanoparticles (TiO2NPs), and their interactions as the PGRs in liquid culture media. Results showed that the growth parameters and photosynthetic pigment content markedly decreased due to polyethylene glycol (PEG)-induced drought stress. The treatment of V. nudicuale plants with TiO2NPs showed a considerably improving effect on pigments synthesis and biomass production during the treatment. However, cultures containing MJA negatively affected the growth parameters and increased the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA). Treatment of plants with PGRs showed a considerable improvement in increasing the synthesis of the phenolic compounds and proline accumulation. Alteration in the activity of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), and polyphenol oxidase (PPO) significantly varied in different treatments. These findings suggest that the recovery treatment with PGRs proved to be very effective in alleviating the adverse effects of drought stress. پرونده مقاله

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8 - Influence of ascorbic acid on growth and micropropagation of Aloe barbadensis Mill.
Behzad Kaviani Aki Ramezani Sayad Ahmad Majd Mojtaba Khorrami Raad

10.30495/ijpp.2024.1935658.1345

Ascorbic acid (AsA) has different roles in plant metabolism. Current research was done to evaluate the effect of various concentrations of AsA on growth and micropropagation of Aloe barbadensis Mill., an important medicinal and ornamental herb, for the first time. In th چکیده کامل

Ascorbic acid (AsA) has different roles in plant metabolism. Current research was done to evaluate the effect of various concentrations of AsA on growth and micropropagation of Aloe barbadensis Mill., an important medicinal and ornamental herb, for the first time. In this regards, results obtained from applying different concentrations of AsA on variables of aerial part length, length and number of root, number of propagule, brownness of medium, and fresh and dry weights of plants were analyzed after 8 weeks. Control plants showed slower growth in aerial parts than plants treated by AsA. Also, leaves were smaller in control plants. Fresh and dry weights in aerial parts were less in control plants than those of treated with AsA. There was a significant increase in produced numbers of propagules in different treatments compared with control treatment. Average number and length of produced roots in plants treated with AsA were more than those of produced in roots of control plants. In addition, brownness of medium and tissue cultures were reduced in plants treated with AsA, due to existence of different phenolic compounds in these plants. Totally, 80 mg L-1 AsA had the highest effect on induction of growth and development of A. barbadensis Mill. in vitro. پرونده مقاله

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9 - Micropropagation of Phalaenopsis circus via direct organogenesis using protocorm-like bodies explant
Hasan Kiaheirati Davood Hashemabadi Behzad Kaviani

10.30495/ijpp.2023.701446

Phalaenopsis orchid has high economic value in the floriculture industry and is one of the most popular orchids in the world. Tissue culture techniques makes it possible to propagate and conserve this species. The aim of the present study was to evaluate the effect of d چکیده کامل

Phalaenopsis orchid has high economic value in the floriculture industry and is one of the most popular orchids in the world. Tissue culture techniques makes it possible to propagate and conserve this species. The aim of the present study was to evaluate the effect of different concentrations of two plant growth regulators (PGRs), namely N-Phenyl-N′-1,2,3-thiadiazol-5-yl-urea (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D), alone and in combination with each other, on the number of protocorm-like bodies (PLBs), leaves, and roots, along with leaf and root lengths of micropropagated Phalaenopsis circus using leaf explants through organogenesis method. Also, correlations between these traits in the Phalaenopsis circus plantlets under study were calculated. Plantlets produced from PLBs were cultured on MS (Murashige and Skoog) basal media enriched with various levels and combinations of TDZ and 2,4-D. The optimal concentrations of the PGRs for micropropagation of Phalaenopsis circus are reported and discussed. The maximum number of PLBs (75.00) was obtained on the medium enriched with 1.00 mg l–1 2,4-D. The highest length of leaf (5.23 cm) was calculated on the medium enriched with 2.00 mg l–1 2,4-D. Concentration of 1.00 mg l–1 TDZ induced the maximum number (6.07) of leaf. The longest root length (4.15 cm) and the largest number of root (4.93) was obtained in medium augmented with 2.00 mg l–1 2,4-D. پرونده مقاله

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10 - Callus Induction and Organogenesis Capacity from Lamina Explant of Petunia × hybrida F1 Induced by BA and NAA
Behzad Kaviani Danesh Kazemi

Plant growth regulators (PGRs) have an important role in callus induction and organogenesis of plant explants cultured in vitro conditions. Callus has a proper potential for plant regeneration through indirect organogenesis and embryogenesis as well suspension culture, چکیده کامل

Plant growth regulators (PGRs) have an important role in callus induction and organogenesis of plant explants cultured in vitro conditions. Callus has a proper potential for plant regeneration through indirect organogenesis and embryogenesis as well suspension culture, genetic transformation and production of secondary metabolites. In current experiment, leaf explants of Petunia × hybrida F1 were cultured on basal Murashige and Skoog (MS) medium supplemented with different concentrations of N6-benzyladenine (BA) (0.25, 0.50 and 1.00 mg l−1) and ɑ-naphthalene acetic acid (NAA) (0.10, 0.20 and 0.30 mg l−1). The maximum fresh weight (5.16 g), dry weight (0.31 g) and volume of callus (24.50 cm3) was obtained in the medium containing 1.00 mg l−1 BA in combination with 0.30 mg l−1 NAA. Leaf explants did not produce callus in medium without PGRs. Plantlets were produced on all callus grown on the media containing PGRs. The most plantlets number was produced on callus induced on medium enriched with 1.00 mg l−1 BA in combination with 0.30 mg l−1 NAA. The plantlets regenerated in vitro with well-developed shoots and roots were successfully established in pots containing peat and perlite (1:1) and grown in a greenhouse within 4 weeks with a 100% survival rate. The regenerated plants were morphologically identical with mother plants and did not show any detectable phenotypic variation. پرونده مقاله

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11 - A Simple and Rapid Method for Micropropagation of Petunia × hybrida F1 'Opera Supreme Pink Morn'
Shahram Mehri Mohammad Nabi Ilkaee Farzin Saeedzadeh

For efficient regeneration of Petunia × hybrida F1 'Opera Supreme Pink Morn', a simple in vitro micropropagation protocol was developed. Axillary bud explants dissected from 30-day-old in vitro-grown seedlings sprouted from hybrid seeds were cultured on Murashige چکیده کامل

For efficient regeneration of Petunia × hybrida F1 'Opera Supreme Pink Morn', a simple in vitro micropropagation protocol was developed. Axillary bud explants dissected from 30-day-old in vitro-grown seedlings sprouted from hybrid seeds were cultured on Murashige and Skoog (MS) medium supplemented with 36 combinations of 6-benzylaminopurine (BA; 0.00, 0.50, 1.00, 2.00, 3.00 and 5.00 mg l-1) and α-naphthaleneacetic acid (NAA; 0.00, 0.10, 0.50, 1.00, 1.50 and 3.00 mg l-1). The BA alone at 0.50 or 2.00 mg l-1 was found to be best for shoot length, shoot number, node number, and leaf number than other concentrations of BA and BA in combination with NAA. The highest number of shoots (8.44) and the maximum average shoot length (13.16 cm) were recorded on MS medium supplemented with 0.50 mg l-1 BA without NAA. Root length (5.20 cm) and root number (8.77) were the maximum in the medium containing 0.10 mg l-1 NAA. The plantlets regenerated in vitro with well-developed shoots and roots were successfully established in pots containing peat and perlite and grown in a greenhouse within 4 weeks with a 100% survival rate. The regenerated plants were morphologically identical with donor plants and did not show any detectable phenotypic variation. Overall, BA at 0.50 or 2.00 mg l-1 had a better effect on shoot system than other concentrations of BA and BA in combination with NAA. NAA induced more root formation and root growth than BA. پرونده مقاله

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12 - Micropropagation of English Yew, an Ornamental-Medicinal Tree
Ali Sahari Moghadam Behzad Kaviani Ali Mohammadi Torkashvand Vahid Abdossi Alireza Eslami

An in vitro propagation method of English yew (Taxus baccata L.) through organogenesis method using kinetin (Kin) and indole 3-butyric acid (IBA) as plant growth regulators and apical bud as explant is presented. Apical buds excised from mother plants were inoculated on چکیده کامل

An in vitro propagation method of English yew (Taxus baccata L.) through organogenesis method using kinetin (Kin) and indole 3-butyric acid (IBA) as plant growth regulators and apical bud as explant is presented. Apical buds excised from mother plants were inoculated on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of Kin (0.00, 0.50, 1.00 and 2.00 mg l–1) as a cytokinin and IBA (0.00, 0.50, 1.00 and 2.00 mg l–1) as an auxin. Results showed that the highest number of node (6.75) was obtained on MS medium containing 2.00 mg l–1 Kin. The maximum shoot number (5.00) was obtained on MS medium supplemented with 1.00 mg l–1 Kin together with 1.00 mg l–1 IBA. The largest number of root (6.50) was produced on explants grown on medium enriched with 2.00 mg l–1 Kin together with 1.00 mg l–1 IBA. Plantlets were transferred to pots filled with perlite and peat moss in equal proportions for acclimatization. These plantlets were acclimated and successfully established in cultivation beds. . پرونده مقاله

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13 - In Vitro Propagation of Cephalanthera rubra (L.) Rich., an Endangered Orchid, Using 2,4-D, NAA and BA
Mahdi Zargar Azad Behzad Kaviani Shahram Sedaghathoor

Orchids are universally popular due to the wide range of colors, sizes, shapes, and scents in their flowers. The demand for orchids as cut flowers and pot plants is increasing in the flower market. Seed germination of orchids is comparatively low in the wild as they typ چکیده کامل

Orchids are universally popular due to the wide range of colors, sizes, shapes, and scents in their flowers. The demand for orchids as cut flowers and pot plants is increasing in the flower market. Seed germination of orchids is comparatively low in the wild as they typically require fungal symbionts during their germination. Micropropagation of orchids is a major approach to orchid conservation and commercial production. Cephalanthera rubra (L.) Rich., one of Iran’s endangered orchid species, has been cloned using leaf as explant, Murashige and Skoog (MS) as culture medium, and α-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) (both at the concentrations of 0, 0.1, 0.2, 0.3 and 0.4 mg L–1) as well 6-benzyladenine (BA) (at 0, 1, 2, 3 and 4 mg L–1) as plant growth regulators (PGRs). In order to initiate an axenic culture, the disinfection of leaf explants was performed with sodium hypochlorite and mercury chloride. The highest number of shoots (4.33) was obtained in medium enriched with 4 mg L–1 BA. Maximum stem length (4.73 cm), leaf number (5.33) and node number (2.86) was obtained in medium supplemented with 3 mg L–1 BA. The largest number of root (5) and the highest length of root (4.83 cm) was produced on medium augmented with 0.3 mg L–1 NAA. Rooted plantlets were transferred to pots filled with peat and perlite in 1:1 proportion and acclimatized to ambient greenhouse conditions with an average of 90% survival rate. This is the first report on the micropropagation of C. rubra (L.) Rich. پرونده مقاله

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14 - An Efficient In Vitro Propagation, Antioxidant and Antimicrobial Activities of Aphyllorchis Montana (Reichenb.f.)
Ganesan Mahendran

An in vitro plant regeneration protocol was successfully established in Aphyllorchis Montana , a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-TM medium was found to be the best followed by K چکیده کامل

An in vitro plant regeneration protocol was successfully established in Aphyllorchis Montana , a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-TM medium was found to be the best followed by KC medium. After 40 days, all the media turned brown and the growths of the protocorms were arrested. Activated charcoal, 1 g/l in half strength BM-TM was found to be suitable for further development of protocorms. Half strength BM-TM medium was supplemented with different growth regulators either individually or in combinations for multiplication of shoots. Of the five cytokinins tested, TDZ at 6.8 μM was found to be most effective for multiple shoot induction yielding 17.24 ± 0.27 shoots after 10 weeks of culture. Addition of low concentration of NAA (1.3 μM) in MS medium supplemented with the cytokinin TDZ (6.8 μM) favoured shoot multiplication. A mean number of 27.56 ± 0.54 shoots with 3.92 ± 0.11 number of roots were produced per explant. The response of the seed derived protocorm to the different types of organic additives viz., peptone and yeast extract and coconut water was also evaluated. The addition of these organic additives to the medium containing TDZ enhanced the number of shoot regeneration. The plantlets were acclimatized in plastic pots containing sterilized vermiculite. The survival rate was 100 % when maintained in the culture room condition (25 ± 2 °C). Screening of the antibacterial, antioxidant activity and estimation of total phenolics and flavonoid content of methanolic extracts of micropropagated plants were also carried out and compared with that of the wild-grown plants. In all the tests, methanolic extract from wild-grown plants showed higher antioxidant, antimicrobial activity, total phenolics and flavonoid content than in vitro propagated plants. پرونده مقاله

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15 - Somatic Embryogenesis and Plant Regeneration from Embryonic Axes and Cotyledons Explants of Tea (Camellia sinenesis L.)
B. Kaviani

In the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-D) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (Camellia sinenesis L.). Embryonic axes and cotyledons explants were dissected from the seeds. Explants were cultured on چکیده کامل

In the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-D) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (Camellia sinenesis L.). Embryonic axes and cotyledons explants were dissected from the seeds. Explants were cultured on Murashige and Skoog (MS) medium containing 0, 1 and 5 mM 2, 4-D alone for embryonic axes and 0, 1 and 5 mM 2, 4-D along with 0 and 0.5 mM IBA for cotyledons. Embryos were observed in embryonic axes explants cultured on MS medium containing 1mM 2, 4-D. No somatic embryos were seen on cotyledons explants. پرونده مقاله

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16 - Micropropagation of Rosa canina Through Axillary Shoot Proliferation
Mahboubeh Davoudi Pahnekolayi Ali Tehranifar Leila Samiei Mahmoud Shoor

In vitro propagation of rose has played a very important role in rapid multiplication of species with desirable traits and production of healthy and disease-free plants. Micropropagation using nodal segments of Rosa canina under different combinations of BAP, GA3 and NA چکیده کامل

In vitro propagation of rose has played a very important role in rapid multiplication of species with desirable traits and production of healthy and disease-free plants. Micropropagation using nodal segments of Rosa canina under different combinations of BAP, GA3 and NAA on MS and VS, medium was investigated. The results showed that the highest shoot proliferation was obtained on VS medium containing 2 mg/L BAP without any GA3 and NAA. Furthermore the highest root regeneration obtained in half strength VS medium. The present investigation recommended a practiciable in vitro plant protocol for R. canina as an important step for successful implementation of biotechnological techniques for rose improvement in Iran. پرونده مقاله

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17 - Effect of BAP and NAA on Micropropagation of Caladium bicolor (Aiton) Vent., an Ornamental Plant
Shima Seydi Naser Negahdar Raziye Taghizadeh Andevari Mohammad Hossein Ansari Behzad Kaviani

Leaf explants of kaladium dwubarwneCaladium bicolor(Aiton) Vent., were cultured in vitro on MS media supplemented with 25 different concentrations of BAP and NAA in order to determine the appropriate concentrations for micropropagation. All combinations induced callus f چکیده کامل

Leaf explants of kaladium dwubarwneCaladium bicolor(Aiton) Vent., were cultured in vitro on MS media supplemented with 25 different concentrations of BAP and NAA in order to determine the appropriate concentrations for micropropagation. All combinations induced callus formation on explants. Callus production on leaves explants grown on control medium was very low. The medium enriched with 4 mg l-1 BAP + 0.5 mg l-1 NAA was the most effective for callus formation. The highest number of shoots (6.43 per explant) and roots (5.56 per explant) were regenerated on media containing 1 mg l-1 BAP + 0.5 mg l-1 NAA and 3 mg l-1 BAP + 0.5 mg l-1 NAA, respectively. The regenerated plantlets were grown in a greenhouse and acclimatized successfully. پرونده مقاله

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18 - The Effect of Different Concentrations of Plant Growt Regulators on Micropropagation of Kalanchoe blossfeldiana cv. White
Behzad Kaviani Davood Hashemabadi Mohaddeseh Kordi

Shoot tips from actively growing, greenhouse maintained plants of Kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on Murashige and Skoog (MS) basal medium supplemented with NAA and BA, both in concentrations of 0.00, 0.50, 1.00 چکیده کامل

Shoot tips from actively growing, greenhouse maintained plants of Kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on Murashige and Skoog (MS) basal medium supplemented with NAA and BA, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. Results showed that the maximum plantlets height (7.012 cm), node number (4.516), root number (8.860) and root length (10.160 cm) were obtained in MS medium containing 1 mg l-1 BA + 1 mg l-1 NAA. Maximum shoot number (5.886), leaf number (8.980) and proliferation index (1.791) were calculated in medium supplemented with 1 mg l-1 BA + 0.5 mg l-1 NAA. Minimum plantlets height (1.988 cm), node number (1.283), root number (2.720), root length (3.016 cm), shoot number (1.221), leaf number (2.015) and proliferation index (0.405) were obtained in medium without BA and NAA (control). Fresh and dry weights of plantlets were calculated, too. About 85% of the micropropagated plantlets were established successfully in acclimatization medium containing peat, perlite and sand (1:1:1). Regenerated plantlets were morphologically identical with mother plants. پرونده مقاله

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19 - Effect of Pre-Harvest Application of Gibberellic Acid and Ethephon on the Quality of Table Grape
M. Zahedi S. Mortazavi N. Moallemi V. Abdossi

In this study the effects of plant growth regulators including; GA3 (0 and 50 mg/l) and ethephon (0 and 500 mg/l) were studied on the quality properties of two grape cultivars (Perlette and Yaghuti). At the harvest time, some physicochemical characteristics such as frui چکیده کامل

In this study the effects of plant growth regulators including; GA3 (0 and 50 mg/l) and ethephon (0 and 500 mg/l) were studied on the quality properties of two grape cultivars (Perlette and Yaghuti). At the harvest time, some physicochemical characteristics such as fruit cluster weight, fruit diameter, length, volume and L/D ratio, flesh firmness, fresh weight, TA, pH, TSS and fruit color were measured at the harvest time. Results showed that fruits treats with GA3 had the highest, cluster weight, fruit diameter, length, volume and L/D ratio compared to ethephon, although pH and flesh firmness showed no differences between the treatments. None of treatments affected negatively the quality of the fruit in term of TSS. Using of GA3+Ethephon treatment significantly increased cluster weight, length, volume, L/D ratio and fresh weight in both cultivars. Ethephon had no significant effect on the fruit size, but promoted berry softening and its effect was different for studied quality parameters. پرونده مقاله

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20 - Effect of Salicylic Acid, Citric Acid and Ascorbic Acid on Post-harvest Quality and Vase Life of Gerbera (Gerbera jamesonii) Cut Flowers
Mona Mehdikhah Rasoul Onsinejad Mohammad Nabi Ilkaee Behzad Kaviani

Cut gerbera (Gerbera jamesonii) flowers are sensitive to microbial contamination and have short vase life. The effect of salicylic acid, citric acid and ascorbic acid (50, 100 and 200 mg l-1) was evaluated on vase life of gerbera flowers. Changes in vase life, water abs چکیده کامل

Cut gerbera (Gerbera jamesonii) flowers are sensitive to microbial contamination and have short vase life. The effect of salicylic acid, citric acid and ascorbic acid (50, 100 and 200 mg l-1) was evaluated on vase life of gerbera flowers. Changes in vase life, water absorption, and bacterial population in stem and vase solution, also biochemical characteristics such as protein concentration, lipid peroxidation level and enzymes activity such as superoxide dismutase and peroxidase were measured and compared with the control. Results showed that the maximum vase life (11.31 and 11.21 days) was achieved in 100 mg l-1 of both citric acid and salicylic acid, respectively. The vase life of control cut flowers was 5.80 days. Most solution uptake (0.907 ml g-1 F.W) was obtained in 100 mg l-1 of citric acid, too. The least bacterial colonies in stem end (151.00) and vase solution (66.33) was obtained in 100 mg l-1 citric acid. Differences between the content of bacterial colonies in vase solution containing 200 mg l-1 citric acid and 100 and 200 mg l-1 salicylic acid was not significant with 100 mg l-1 citric acid. The lowest content (46.04 and 46.21 nmol g-1 F.W.) of lipid peroxidation or MDA content was obtained from cut flowers treated with 200 mg l-1 of citric acid and 100 mg l-1 salicylic acid, respectively. Maximum activity of the peroxidase (0.063 mmol g-1 F.W.) and superoxide dismutase (40.80 nmol g-1 F.W.) enzymes was observed in 200 mg l-1 of citric acid. پرونده مقاله

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21 - Effect of Gibberellic Acid Pulsing and Sucrose Continuous Treatment on Some Qualitative Characteristics of Cut Rose Flower cv. Velvet
Zahra Ahmadi Rahim Naghshiband Hassani

The aim of this study was the better assessment of the relationship ofpulsing treatment of gibberellic acid as an anti-senescence agent and holdingin sucrose as a carbohydrate supply resource on improving some qualitativeand physiological attributes of cut rose flower c چکیده کامل

The aim of this study was the better assessment of the relationship ofpulsing treatment of gibberellic acid as an anti-senescence agent and holdingin sucrose as a carbohydrate supply resource on improving some qualitativeand physiological attributes of cut rose flower cv. Velvet. Hence, anexperiment involving the pulsing treatment with gibberllic acid (GA3) at 0,20, 40 and 60 mg L-1, for 24 hours, and holding them in sucrose at 0, 2 and3% with 250 mg L-1 of 8- HQS as an antimicrobial agent for all holdingtreatments was conducted. The study was performed as a factorial experimentbased on a randomized completely design (RCD) with three replication foreach combination treatment. Applying GA3 pulse treatment alone at all concentrationsincreased significantly vase life and its effect enhanced withsucrose holding at 2 and 3% compared with control. The effect of GA3pulsing in increasing of stem relative fresh weight (RFW) and solutionuptake (SU) was hastened by sucrose holding treatment at 2 and 3%.Highest amount of flower opening and petal water content during vase lifeperiod was observed in 60 mg L-1 of GA3 pulsing and sucrose 3% holdingtreatment which had been longer flower diameters and greater petals.Gibberellic acid pulsing alone and along with sucrose holding treatment atall concentrations caused to prevention of leaf chlorophyll degradationcompared with control. In conclusion, GA3 pulsing at 40 mg L-1 along withsucrose 2% holding treatment had a significant effect on improving vase lifeand other qualitative attributes of cut rose flower cv. Velvet. پرونده مقاله

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22 - تأثیر مقادیر مختلف کلرمکوات کلرید و تراکم بوته در کنترل خوابیدگی گندم
محمد صادقی حمیدرضا میری

افزایش تراکم بوته با هدف بهبود عملکرد دانه ممکن است سبب خوابیدگی (ورس) درگندم شود، که این خود به کاهش کارایی فنوسنتز و در نتیجه به کاهش عملکرد دانه منجر می شود. در یک تحقیق مزرعه ای با آزمایش فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با سه تکرار در محل ایستگاه مرکز تحقی چکیده کامل

افزایش تراکم بوته با هدف بهبود عملکرد دانه ممکن است سبب خوابیدگی (ورس) درگندم شود، که این خود به کاهش کارایی فنوسنتز و در نتیجه به کاهش عملکرد دانه منجر می شود. در یک تحقیق مزرعه ای با آزمایش فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با سه تکرار در محل ایستگاه مرکز تحقیقات کشاورزی فارس (زرقان) تأثیر چهارتراکـم بوته 200، 300، 400 و 500 بوته در مترمربع و سه غلظت صفر، 1500 و 3000 گرم در هکتار کلرمکوات کلرید (CCC) بر خوابیدگی و برخی صفات مورفولوژیک گندم رقم روشن (از ارقام محلی پابلند و حساس به خوابیدگی) مورد بررسی قرار گرفت. نتایج نشان داد که افزایش تراکم بوته از 200 به 500 بوته در مترمربع باعث افزایش ارتفاع بوته، کاهش وزن دانه، افزایش عملکرد دانه و افزایش خوابیدگی تا 70% شد و تأثیر معنی داری بر سایر صفات نداشت. همچنین کاربرد CCC باعث کاهش ارتفاع بوته، کاهش میزان ورس، کاهش وزن خشک و میزان فتوسنتز بعد از گرده افشانی شد، اما تأثیر معنی داری بر سایر صفات نداشت. در مجموع نتایج نشان داد که در تیمار تراکم 400 بوته در متر مربع و غلظت 3000 گرم کلر مکوات کلرید کمترین میزان خوابیدگی در رقم گندم روشن مشاهده شد و با توجه به عملکرد مناسب دانه، این تیمار قابل توصیه می‌باشد. پرونده مقاله

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23 - The Effects of Foliar Application of Salicylic Acid and Ascorbic Acid on Morpho-Physiological Traits of Pistachio Seedlings under Drought Stress
Elham Ebrahimpour Bahman Panahi Alireza Talaie Iraj Tavassolian

10.22034/jon.2020.1873805.1063

This study was carried out to investigate the effects of foliar spray of salicylic acid and ascorbic acid on some morpho-physiological traits of Pistacia vera cv. Badami-zarand under drought stress. The experiment was conducted during 2017-2018 in the greenhouse conditi چکیده کامل

This study was carried out to investigate the effects of foliar spray of salicylic acid and ascorbic acid on some morpho-physiological traits of Pistacia vera cv. Badami-zarand under drought stress. The experiment was conducted during 2017-2018 in the greenhouse condition, in a factorial randomized complete block design with three replications on pot plants. The soil texture of experimental pots was sandy-loam. Moreover, the three factors were considered as follows: first factor, drought stress treatments at three levels; control (100% usable moisture content), moderate stress (60% usable moisture content), and severe stress (30% usable moisture content); second factor, salicylic acid, at three levels (0, 0.5mM, and 1mM); and ascorbic acid at three levels (0, 0.5mM, and 1mM). At the end of experiment related physiological, morphological, and biochemical traits related to 6-month-old seedlings were assessed including the fresh and dry weight of leaf, stem and root, number of leaves, leaf thickness, diameter and length of stem, length of root, relative water content, rate of electrolyte leakage, rate of proline accumulation, rate of glycine betaine accumulation, chlorophyll a content, chlorophyll b content, and total chlorophyll. Generally, the results indicated that the value of morphological traits showed a significant reduction in stress treatments. In this experiment, it was found that the application of salicylic acid and ascorbic acid can also reduce the adverse effects of moderate drought stress. Furthermore, it was identified that the response of plant to salicylic acid treatment is similar to ascorbic acid. In general, the application of salicylic acid with a concentration of 0.5mM and ascorbic acid with concentrations of 0.5mM and 1mM is recommended to improve the adaptation of pistachio seedlings under moderate stress. پرونده مقاله

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24 - In Vitro Callogenesis and Regeneration of Cucumber Plants from Hypocotyl and Cotyledon
ABOUZAR ASADI

10.30486/roce.2023.709432

In Vitro Callogenesis and Regeneration of Cucumber Plants from Hypocotyl and Cotyledon Abouzar Asadi1* 1- Assistant Professor, Plant Improvement and Seed Production Center and Department of Plant Genetic and Production Engineering, Azad University, Isfahan, Iran. چکیده کامل

In Vitro Callogenesis and Regeneration of Cucumber Plants from Hypocotyl and Cotyledon Abouzar Asadi1* 1- Assistant Professor, Plant Improvement and Seed Production Center and Department of Plant Genetic and Production Engineering, Azad University, Isfahan, Iran. *Corresponding email address: asadi.abzr@gmail.com Received: 5 November October 2022 Accepted: 18 January 2023 Abstract In vitro culturing of cotyledon and hypocotyl can serve various purposes such as gene transfer and asexual reproduction. This study aimed to regenerate plants from cotyledon and hypocotyl explants of cucumber cultivars, the Iranian landrace Isfahan and Beta Alpha F1 hybrid. The experiment was carried out in the tissue culture laboratory of Azad University of Isfahan in 2023. It employed a factorial completely randomized design with two genotypes and 10 media in the three replicate dishes. The media consisted of MS supplemented with BAP (1, 2, 3, and 4 mg/l) combined with NAA (0.05, 0.1, 0.2, and 0.5 mg/l). The results showed that callus was successfully induced in both cotyledon and hypocotyl explants in both genotypes. The highest percentages of regeneration from cotyledons were observed in 3 mg/l BAP and 0.5 mg/l NAA and 4 mg/l BAP and 0.1 mg/l NAA, with 65% and 60%, respectively, for the Isfahan genotype and in 1 mg/l BAP and 0.2 mg/l NAA and 1 mg/l BAP and 0.5 mg/l NAA, both exhibiting a 60% regeneration rate for Beta Alpha. Furthermore, when Isfahani and Beta-alpha’s hypocotyl explants were cultured in MS supplemented with 2 mg/l BAP and 0.1 mg/l NAA, and 1 mg/L BAP and 0.2 mg/L NAA had 30% and 55% rate of regeneration, respectively. The present findings indicate that induced callus or shoot regeneration depended on genotype and explants. Each genotype requires specific callogenesis and regenerated media to achieve desirable results. Overall, the results suggest that cotyledons in the Isfahan genotype and hypocotyls in the Beta-alpha variety exhibited better regeneration when tested with these plant growth regulators. پرونده مقاله

فهرست مقالات plant growth regulators

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