Micropropagation of English Yew, an Ornamental-Medicinal Tree
محورهای موضوعی : مجله گیاهان زینتی
Ali Sahari Moghadam
1
(
Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
)
Behzad Kaviani
2
(
Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran
)
Ali Mohammadi Torkashvand
3
(
Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
)
Vahid Abdossi
4
(
Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
)
Alireza Eslami
5
(
Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran
)
کلید واژه: plant growth regulators, Forest trees, <i> In vitro</i> multiplication, Threatened ornamental plant,
چکیده مقاله :
An in vitro propagation method of English yew (Taxus baccata L.) through organogenesis method using kinetin (Kin) and indole 3-butyric acid (IBA) as plant growth regulators and apical bud as explant is presented. Apical buds excised from mother plants were inoculated on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of Kin (0.00, 0.50, 1.00 and 2.00 mg l–1) as a cytokinin and IBA (0.00, 0.50, 1.00 and 2.00 mg l–1) as an auxin. Results showed that the highest number of node (6.75) was obtained on MS medium containing 2.00 mg l–1 Kin. The maximum shoot number (5.00) was obtained on MS medium supplemented with 1.00 mg l–1 Kin together with 1.00 mg l–1 IBA. The largest number of root (6.50) was produced on explants grown on medium enriched with 2.00 mg l–1 Kin together with 1.00 mg l–1 IBA. Plantlets were transferred to pots filled with perlite and peat moss in equal proportions for acclimatization. These plantlets were acclimated and successfully established in cultivation beds. .
یک روش تکثیر درون‌شیشه‌ای سرخدار انگلیسی (Taxus baccata L.) طی روش اندام‌زایی با استفاده از کینتین (Kin) و ایندول-3-بوتیریک اسید (IBA) به عنوان تنظیم‌کننده‌های رشد گیاهی و جوانه رأسی به عنوان ریزنمونه ارائه می‌شود. جوانه‌های رأسی بریده‌شده از گیاهان مادری روی محیط موراشیگ و اسکوگ (MS) مکمل‌شده با غلظت‌ها و ترکیب‌های مختلف Kin (1، 0.5، 0 و 2 میلی‌گرم بر لیتر) به عنوان یک سیتوکینین و IBA (1، 0.5، 0 و 2 میلی‌گرم بر لیتر) به عنوان یک اکسین قرار گرفتند. نتایج نشان داد که بیشترین تعداد گره (6.75) روی محیط MS حاوی 2 میلی‌گرم بر لیتر Kin به دست آمد. بیشترین تعداد سرشاخه (5) روی محیط MS مکمل‌شده با 1 میلی‌گرم بر لیتر Kin همراه با 1 میلی‌گرم بر لیتر IBA به دست آمد. بیشترین تعداد ریشه (6.50) روی ریزنمونه‌های رشدیافته روی محیط غنی‌شده با 2 میلی‌گرم بر لیتر Kin همراه با 1 میلی‌گرم بر لیتر IBA تولید شد. گیاهچه‌ها به گلدان‌های پرشده با پرلایت و پیت موس به نسبت مساوی برای سازگاری منتقل شدند. این گیاهچه‌ها سازگار شدند و به طور موفقیت‌آمیزی در بسترهای کشت استقرار یافتند.
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