Study of the antileukemic activity of Mimosa caesalpiniifolia Benth. ethanolic extract and fractions

Subject Areas : Phytochemistry: Isolation, Purification, Characterization

Gabriele Taumaturgo Mororó ¹ , José Roberto de Oliveira Ferreira ² , Michel Mualén de Morais Alves ³ , Nayana Bruna Nery Monção ⁴ , Laís Campos Teixeira de Carvalho-Gonçalves ⁵ , Antônia Maria das Graças Lopes Citó ⁶ , Paulo Michel Pinheiro Ferreira ⁷ , Fernando Aécio de Amorim Carvalho ⁸ , Juan Carlos Ramos Gonçalves ⁹

1 - Núcleo de Pesquisas em Plantas Medicinais, Universidade Federal do Piauí, Teresina-PI, Brasil
2 - Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Piauí, Teresina-PI, Brasil
3 - Núcleo de Pesquisas em Plantas Medicinais, Universidade Federal do Piauí, Teresina-PI, Brasil
4 - Departamento de Química, Universidade Federal do Piauí, Teresina-PI, Brasil
5 - Centro de Biotecnologia, Universidade Federal da Paraíba, João Pessoa-PB, Brasil
6 - Departamento de Química, Universidade Federal do Piauí, Teresina-PI, Brasil
7 - Departamento de Biofísica e Fisiologia, Laboratório de Cancerologia Experimental, Universidade Federal do Piauí, Teresina-PI, Brasil
8 - Núcleo de Pesquisas em Plantas Medicinais, Universidade Federal do Piauí, Teresina-PI, Brasil
9 - Núcleo de Pesquisas em Plantas Medicinais, Universidade Federal do Piauí, Teresina-PI, Brasil

Received: 2018-02-24 Accepted : 2018-09-20 Published : 2018-09-01

Keywords: Medicinal Plants, Cytotoxicity, Leukemia, Betulinic acid, <i>M. caesalpiniifolia</i> Benth,

Abstract :

Mimosa caesalpiniifolia Benth. is a native plant to northeastern Brazil, traditionally used in folk medicine, with several pharmacological activities reported including antibacterial, anti-inflammatory, and antitumor. The present study evaluated the antileukemic potential of M. caesalpiniifolia Benth. ethanolic extract (EtOH) and its n-hexanic (HexF) and dichloromethane (DCMF) fractions. Previous analysis by our team revealed the constituents of high relative abundance in EtOH, HexF, and DCMF, like phytol (11.7%), lupeol (14.7%), and betulinic acid (70.3%), respectively. In the MTT cell viability test, EtOH, HexF, and DCMF induced dose-dependent cytotoxicity in human chronic myeloid cells (K562), with IC50 of 153.6 ± 0.1, 118.40 ± 0.2, and 40.0 ± 0.1 μg/mL, respectively (p <0.05). Additionally, DCMF (6-800 μg/mL) presented minor toxicity against normal human erythrocytes and murine macrophage cells. DCMF induced similar antileukemic effects (IC50=64.2 ± 5.0 μg/mL) against human acute myeloid cells (HL-60). However, it did not exert antitumor activity on murine sarcoma (S180) cells (p >0.05).

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