Bioactivity of essential oil from Hertia cheirifolia L. flowers in the control of bacteria

Subject Areas : Essential Oils: Classical and Advanced Techniques for Isolation and Biological Activities

Kaouther Majouli ¹ , Abderraouf Kenani ²

1 - Laboratory of Biochemistry, Research Unit: UR 12ES08 “Cell Signaling and Pathologies”, Faculty of Medicine, University of Monastir, Tunisia
2 - Laboratory of Biochemistry, Research Unit: UR 12ES08 “Cell Signaling and Pathologies”, Faculty of Medicine, University of Monastir, Tunisia

Received: 2017-05-30 Accepted : 2017-04-23 Published : 2017-03-01

Keywords: Hydrodistillation, Antibacterial activity, Essential oil, <i>Hertia cheirifolia</i>,

Abstract :

To date, numerous studies have demonstrated beneficial properties of essential oils in the prevention and treatment of a variety of diseases. The current report evaluated, in vitro, the antibacterial activity of essential oil from the flowers of Hertia cheirifolia L. This oil was obtained by hydrodistillation using a Clevenger apparatus. The antibacterial activity of the water-distilled essential oil of H. cheirifolia L. was evaluated against Gram positive microorganisms, namely Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633, Bacillus licheniformis and Gram negative bacterial strains, namely Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, Salmonella enteric IPC 8039 and Salmonella typhimirium using the microdilution method in sterile 96- well microplates. The numerical values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined. Regarding the obtained results, the highest antibacterial activities of essential oils were found against Staphylococcus aureus ATCC 6538 and Bacillus licheniformis both having an identical MIC value of 0.078 mg/mL. Our study showed that the use of the essential oil of H. cheirifolia flowers could be considered as a natural source to inhibit the growth of tested pathogenic bacteria.

References:

Abrassat, J.L., 1988. A Thousand and One Virtues of Essential Oils, Ed. Guy Tredaniel.

Afsharypuor, S., Jahromy, M.M., Rahiminezhad, M.R., 2000. Investigation of the volatile oil of Hertia angustifolia (DC.) O. Kuntze. Daru J. Pharm. Sci. 8, 7-8.

Abrassat, J.L., 1988. A Thousand and One Virtues of Essential Oils, Ed. Guy Tredaniel.

Afsharypuor, S., Jahromy, M.M., Rahiminezhad, M.R., 2000. Investigation of the volatile oil of Hertia angustifolia (DC.) O. Kuntze. Daru J. Pharm. Sci. 8, 7-8.

Ammar, S., Edziri, H., Mahjoub, M.A., Chatter, R., Bouraoui, A., Mighri, Z., 2009. Spasmolytic and anti-inflammatory effects of constituents from Hertia cheirifolia. Phytomedicine 16, 1156-1161.

Attia, S., Grissa, K.L., Mailleux, A.C., Heuskin, S., Lognay, G., Hance, T., 2012. Acaricidal      activities of Santolina africana and Hertia cheirifolia essential oils against the two-spotted      spider mite (Tetranychus urticae). Pest Manag. Sci. 68, 1069-1076.

Baudoux, D., 2002.  The Aromatherapy: To Be Treated by the Essential Oils, Ed. Amyris,      Brussels, pp. 17-36.

Bruneton, J., 1987. Element of Phytochemistry and Pharmacognosy. (First Ed.), Tech and      Doc, Lavoisier, Paris, pp. 584.

Bruneton, J., 1993. Pharmacognosy, Phytochemistry, Medicinal use the, 2nd ed. Tech and Doc,      Lavoisier, Paris.

Carson, C.F., Mee, B.J., Riley, T.V., 2002. Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage and salt tolerance assays and electron microscopy. Antimicrob. Agents Chemother. 46, 1914-1920.

Cintia, S.G.K., Smania, J.A., Pedrosa, R.C., Ferreira, S.R.S., 2007. Antioxidant and antimicrobial activities of shiitake (Lentinula edodes) extracts obtained by organic solvents and supercritical fluids. J. Food Eng. 80, 631-638.

Clevenger, J.F., 1928. Apparatus for the determination of volatile oil. J. Am. Pharm. Assoc. 17, 341-346.

Cox, S.D., Mann, C.M., Markham, J.L., Bell, H.C., Gustafson, J.E., Warmington, J.R., Wyllie, S.G., 2000. The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). J. Appl. Microbiol. 88, 170-175.

Davidson, P.M., 1997. Chemical preservatives and natural antimicrobial compounds. In: Doyle, M.P., Beuchat, L.R., Montville, T.J., (Eds.), Food Microbiology. ASM, Washington, pp. 520-556.

Dorman, H.J.D., Deans, S.G., 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J. Appl. Microbiol. 88, 308-316.

Franchomme, P., Jollois, R., Penoel, D., 2001.  Aromatherapy Exactly: Encyclopedia of the therapeutic use of aromatic extracts, Ed. Roger Jollois.

Majouli, K., Besbes Hlila, M., Hamdi, A., Flamini, G., Ben Jannet, H., Kenani, A., 2016a. Antioxidant activity and α-glucosidase inhibition by essential oils from Hertia cheirifolia (L.). Ind. Crop Prod. 82, 23-28.

Majoul,i K., Hamdi, A., Msaada, K., Kenani, A., 2016b. A bioactivity guided study on the antibacterial activity of Hertia cheirifolia L. extracts. Microb. Pathogen. 1-6.

Majouli, K., Mahjoub, M.A., Rahim, F., Hamdi, A., Wadoode, A., Besbes Hlila, M., Kenani, A., 2017. Biological properties of Hertia cheirifolia L. flower extracts and effect of the nopol on α-glucosidase. ‎ Int. J. Biol. Macromol. 95, 757-761.

Mohammadhosseini, M., Sarker, S.D., Akbarzadeh, A., 2017. Chemical composition of the essential oils and extracts of Achillea species and their biological activities: A review. J. Ethnopharmacol. 199, 257-315.

Ruberto, G., Baratta, M.T., 2000. Antioxidant activity of selected essential oil components in two lipid model systems. Food Chem. 69, 167-174.

Sanon, A., Garba, M., Auger, J., Huignard, J., 2001. Analysis of the insecticidal activity of methylisothiocyanate on Callosobruchus maculatus (Coleoptera: Bruchidae) and its parasitoid Dinarmus basalis (Rondani) (Hymenoptera: Pteromalidae). J. Stored Prod. Res. 38, 129-138.

Sharifi-Rad, J., Sureda, A., Tenore, G.C., Daglia, M., Sharifi-Rad, M., Valussi, M., 2017. Biological activities of essential oils: From plant chemoecology to traditional healing     systems: A review. Molecules 22, 70.

Sharma, P.R., Dilip, M.M., Muthiah, S., Pal, H.C., Shahi, A.K., Saxena, A.K., Qazi, G.N., 2009. Anticancer activity of an essential oil from Cymbopogon flexuosus. Chem. Biol. Interact. 179, 160-168.

Yanishlieva, N.V., Marinova, E.M., Gordon, M.H., Raneva, V.G., 1999. Antioxidant activity and mechanism of action of thymol and carvacrol in two lipid systems. Food Chem. 64, 59-66.