پاسخ بیان ژنcateninβو baxدر بافت کبد موش های مبتلا به سرطان ملانوما به یک دوره تمرینات استقامتی همراه با مصرف عصاره گزنه
محورهای موضوعی : فیزیولوژی تجربی و آسیب شناسیجاوید اسماعیل پور 1 , علیرضا براری 2 , احمد عبدی 3 , حسین عابدنطنزی 4
1 - گروه فیزیولوژی ورزشی، واحد آیت الله آملی ، دانشگاه آزاد اسلامی ، آمل,
2 - گروه فیزیولوژی ورزشی واحد آیت الله آملی، دانشگاه آزاد اسلامی ، آمل، ایران.
3 - گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
4 - گروه تربیت بدنی و علوم ورزشی٬ واحد علوم و تحقیقات٬ دانشگاه آزاد اسلامی٬ تهران٬ ایران.
کلید واژه: عصاره گزنه, آپوپتوز, مسیر سیگنالیWNT/beta-catenin, سرطان ملانوما.,
چکیده مقاله :
زمینه و هدف: هدف از تحقیق حاضر بررسیپاسخ بیان ژن cateninβ و bax در بافت کبد موش های مبتلا به سرطان ملانوما به یک دوره تمرینات هوازی همراه با مصرف عصاره گزنه بود.
مواد و روش ها: در این تحقیق 20 سر موش صحرایی نر بالغ به صورت تصادفی به 4 گروه شامل گروههای: کنترل ، تمرین، عصاره و تمرین+عصاره تقسیم شدند. برنامه تمرین شامل 30 دقیقه دویدن روی تردمیل بدون شیب و با سرعت 16 متر در دقیقه برای هفته اول بود و هر هفته یک متر بر دقیقه اضافه شد تا در هفته هشتم به 22 متر بر دقیقه رسید. یک هفته پس از القا سرطان ملانوما، گروه تجربی میزان mg/kg/day 30 عصاره اتانولی گیاه گزنه را به روش خوراکی و به مدت 8 هفته مصرف کردند. برای اندازه گیری میزان بیان ژن cateninβ و bax از روش RT-PCR استفاده شد.
نتایج: تجزیه و تحلیل دادهها نشان داد بیان ژن Beta-catenin در گروه های تجربی نسبت به گروه کنترل افزایش داشت؛ ولی به سطح معناداری نرسید (p=0.103). همچنین نتایج نشان داد که بیان ژن BAX در گروه های تجربی نسبت به گروه کنترل کاهش معناداری داشت(p=0.026).
نتیجه‌گیری: نتایج نشان می دهد که مصرف عصاره گزنه همراه با تمرینات هوازی از طریق کاهش سطوح BAX و افزایش بتا کاتنین در فعال سازی مسیر سیگنالی WNT/beta-catenin و آپوپتوز محرک ایمنی برای جلوگیری از رشد تومور و پیشرفت مرحله سرطان داشته باشد.
Background & Aim: The aim of this study was to investigate the response of beta-cateninand bax gene expression in liver tissue of mice with melanoma to a period of aerobic exercise with nettle extract.
Materials and methods: In this study, 20 adult male rats were randomly divided into 4 groups including: control, exercise, extract and exercise + extract. The training program consisted of 30 minutes of running on a treadmill without a slope at a speed of 16 meters per minute for the first week, and one meter per minute was added every week until it reached 22 meters per minute in the eighth week. One week after melanoma induction, the experimental group consumed 30 mg / kg / day of nettle ethanolic extract orally for 8 weeks. RT PCR was used to measure the expression of beta-cateninand baxgenes.
Results: Data analysis showed that Beta-catenin gene expression was increased in the experimental groups compared to the control group; But did not reach a significant level (p = 0.103). The results also showed that BAX gene expression was significantly reduced in the experimental groups compared to the control group (p = 0.026).
Conclusion: The results show that consumption of nettle extract along with aerobic exercise by reducing BAX levels and increasing beta-catenin in activating the WNT / beta-catenin signal pathway and apoptosis has an immune stimulus to prevent tumor growth and cancer progression.
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