القاء چاقی، عصاره سیر و استویا به‌همراه فعالیت هوازی، بر بیان ژن سمافورین4D و گیرنده‌ی پلکسین-B2 در موش‌های نر صحرائی
محورهای موضوعی : فیزیولوژی تجربی و آسیب شناسی
ملیحه اردکانی زاده
1
,
سید جواد ضیاءالحق
2
1 - دانشگاه دامغان، گروه علوم ورزشی
2 - گروه فیزیولوژی ورزشی واحد شاهرود، دانشگاه آزاد اسلامی ، شاهرود، ایران.
کلید واژه: چاقی, عصاره سیر, عصاره استویا, سمافورینD4, پلکسینB2.,
چکیده مقاله :
زمینه و هدف: ازدیرباز، میوه سیر و برگ استویا در کنار فعالیت بدنی هوازی، به‌عنوان یک ضد التهاب بیان شده‌اند، ازطرفی به‌تازگی سمافورین‌ها به‌عنوان پروتئین‌های اثرگذار بر التهاب سیستم عصبی مرکزی شناسائی شده‌اند. مواد و روش‌ها: تعداد 35 سر رت نژاد ویستار نر چاق شده (شاخص لی) بصورت تصادفی به 7 گروه (پنج تایی)؛ کنترل سالم، چاق، سیر، استویا، هوازی، سیر+هوازی، و استویا+هوازی تقسیم شدند. تمرینات هوازی شامل30 دقیقه/روز، پنج روز/هفته بود، و عصاره سیر و استویا با غلظت 250 میلی‌گرم/کیلوگرم به آب مصرفی روزانه اضافه شد. جهت مطالعات بیان ژن سمافورین 4Dو گیرنده آن بنام پلکسینB2 به روش real-time PCR بافت مغز به آزمایشگاه ارسال شد. از آزمون آنالیز واریانس یک‌طرفه و آزمون تعقیبی توکی جهت تعیین اختلاف بین گروه‌ها استفاده گردید (05/0p≤). نتایج: مقادیر بیان ژن سمافورینD4 گروه چاق نسبت به گروه کنترل سالم افزایش معنی‌دار داشت، و همه گروه‌ها غیر از گروه استویا+هوازی، کاهش معنی‌داری در میزان این ژن نشان دادند. مقادیر پلکسینB2 گروه استویا+هوازی از مقادیر گروه کنترل چاق نیز فراتر بود، و گروه سیر با/بدون فعالیت هوازی مقادیر کمتری نسبت به گروه کنترل سالم و چاق تجربه کردند (05/0>p). نتیجه گیری: به‌نظر می‌رسد که القاء چاقی موجب افزایش بیان ژن سمافورین4D و گیرنده پلکسینB2 می‌گردد، و عصاره سیر و ترکیب آن با فعالیت هوازی اثرات مفیدی بر کاهش التهاب سیستم عصبی مرکزی ایفا می‌کنند.
Background and aim: For a long time, garlic fruit and stevia leaves have been expressed as an anti-inflammatory along with aerobic physical activity, on the other hand, semaphorins have recently been identified as proteins that affect central nervous system inflammation. Materials & Methods: the number of 35 obese male Wistar rats (Lee's index) randomly divided into 7 groups (n=5); healthy control, obese, garlic, stevia, aerobic, garlic+aerobic, and stevia+aerobic. Aerobic exercises consisted of 30 M/D, five D/W, and garlic and stevia extract with a concentration of 250 mg/kg was added to the water consumed daily. In order to study the expression of semaphoring-4D gene and its receptor called plexin-B2, brain tissue was sent to the laboratory by real-time PCR method. One-way analysis of variance and Tukey's post hoc test were used to determine the difference between groups (p≤0.05). Results: The expression levels of semaphoring-D4 gene in the obese group increased significantly compared to the healthy control group, and all groups showed a significant decrease in the amount of this gene, except for the aerobic+stevia group. The values of plexin-B2 in the aerobic+stevia group exceeded the values of the obese control group, and the garlic group with/without aerobic activity experienced lower values than the healthy and obese control group (p<0.05). Conclusion: It seems that induction of obesity increases the expression of semaphoring-4D gene and plexin-B2 receptor, and garlic extract and its combination with aerobic activity have beneficial effects on reducing central nervous system inflammation.
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