اثر تیمار مزمن با پاناکس جینسینگ بر تغییرات هیستوپاتولوژیک ناحیه شکنج دندانه ای هیپوکمپ موش های صحرایی مدل صرع پیلوکارپین
محورهای موضوعی : فیزیولوژی تجربی و آسیب شناسی
مهین گنج خانی
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رضا بیگناه
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1 - دانشگاه علوم پزشکی زنجان
2 - دانشگاه علوم پزشکی زنجان
کلید واژه: صرع مدل پیلوکارپین, پاناکس جینسینگ, هیستوپاتولوژی, هیپوکامپ,
چکیده مقاله :
زمینه و هدف: با توجه به اینکه جوانه زدن فیبرهای خزه ای به عنوان شاخص مهم در ارزیابی شدت صرع لوب گیجگاهی در مدلهای حیوانی محسوب می شود، هدف از مطالعه حاضر بررسی اثرات جینسینگ بر تغییرات هیستو پاتولوژیک ناحیه شکنج دندانه ای هیپوکامپ موشهای صحرائی صرعی شده با پیلوکارپین است.
مواد و روش ها: درمطالعه تجربی حاضر70 سر موش صحرایی بالغ نر نژاد ویستار (Wistar) به طور تصادفی به گروه های مختلف تقسیم شدند. در 4 گروه به منظور مدلسازی صرع، از لیتیوم- پیلوکارپین(mg/kg 127) بصورت تزریق داخل صفاقی استفاده شد. سپس به سه گروه از گروه های صرعی شده، بترتیب جینسینگmg/kg 150 ، والپروات سدیم mg/kg 200 (بعنوان کنترل مثبت) و جینسینگ به همراه والپروات سدیم به مدت سی روز داده شد. پس از اتمام دوره درمان و انجام بیوپسی از هیپوکامپ، جهت ارزیابی تعداد سلولها، رنگ آمیزی نیسل و برای بررسی جوانه زدن نابجای آکسونی، رنگ آمیزی Timm’s انجام گرفت. تجزیه و تحلیل داده ها با استفاده از نرم افزار SPSS و آزمونهای آماری ANOVA انجام شد.
نتایج: مشاهده شد که تیمار با جینسینگ بطور معناداری (p<0.05) در مقایسه با گروه Sham، به بهبود آسیب ناحیه هیپوکامپ کمک نموده است.
نتیجه گیری: تیمار با جینسینگ اثر بهتری در مقایسه با والپروات سدیم بر بهبود و کاهش عوارض ناشی از صرع در هیپوکامپ دارد. بنظر می رسد که جینسینگ، مانع تخریب نورون ها و تولید فیبرهای خزه ای نابجا در صرع شده است.
Objective: Because mossy fiber sprouting is considered an important indicator in assessing the severity of temporal lobe epilepsy in animal models, the purpose of the present study is to investigate the protective effects of ginseng and sodium valproate on histopathologic changes in the dentate gyrus region of the hippocampus in pilocarpine-induced epileptic rats
Methods: In the present experimental study, 70 adult male Wistar rats were randomly divided into different groups. In 4 groups, lithium pilocarpine (127 mg/kg) was used as an intraperitoneal injection to model epilepsy. Then, three groups of epileptic groups were given ginseng 150 mg/kg, sodium valproate 200 mg/kg (as a positive control), and ginseng plus sodium valproate for thirty days, respectively. At the end of the treatment period and after hippocampal biopsy, Nissl staining was performed to evaluate cell number and Timms staining was performed to examine aberrant axonal sprouting. Data analysis was performed using SPSS software and ANOVA statistical tests.
Results: It was observed that ginseng treatment significantly (p<0.05) helped ameliorate the hippocampal damage compared to the epileptic non treatment group.
Conclusion: Ginseng treatment has a better effect than sodium valproate on improving and reducing complications of epilepsy in the hippocampus. It seems that ginseng prevents the destruction of neurons and the production of abnormal mossy fibers in epilepsy.
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