تاثیر یک دوره تمرین هوازی به همراه مصرف مکمل ویتامین C بر گیرنده لپتین و شاخص های استرس اکسیداتیو در بافت قلب موشهای ماده صحرایی مبتلا به آلزایمر
تاثیر تمرین هوازی , ویتامین C بر گیرنده لپتین و استرس اکسیداتیو در بافت قلب موشهای آلزایمر
محورهای موضوعی : فیزیولوژی تجربی و آسیب شناسی
اسیه سید
1
,
مرضیه فلاحیان
2
,
فروغ تجلی
3
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سیده ام البنین قاسمیان
4
1 - گروه فیزیولوژی ورزشی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران
2 - گروه فیزیولوژی ورزشی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران.
3 - گروه فیزیولوژی ورزشی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران.
4 - گروه دامپزشکی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران * .
کلید واژه: آلزایمر, آنزیم‌های ضد اکسایشی, استرس اکسیداتیو, ورزش هوازی, ویتامین C,
چکیده مقاله :
زمینه و هدف: آنزیم‌های ضد اکسایشی اولین خط دفاعی در برابر حمله‌ی انواع رادیکال‌های فعال اکسیژن می‌باشد. آنتی‌اکسیدان‌ها از طریق واکنش با رادیکال‌های آزاد و گونه‌های فعال باعث کاهش یا جلوگیری از استرس اکسیداتیو می‌شوند. مطالعه حاضر با هدف تاثیر یک دوره تمرین هوازی به همراه مصرف مکمل ویتامین C بر گیرنده لپتین، وزن چربی احشایی، BMI و آنزیم‌های ضد اکسایشی در بافت مغز موشهای صحرایی مبتلا به آلزایمر، صورت گرفت. مواد و روش ها: در این مطالعه تجربی، 45 سر موش صحرایی سالمند ماده به طور تصادفی به شش گروه کنترل مبتلا به آلزایمر، مکمل ویتامین C، تمرین هوازی، تمرین هوازی + ویتامین C و کنترل سالم، تقسیم شدند. شاخص‌های لپتین، وزن چربی احشایی و BMI پس از اتمام مداخله و شاخص‌های MDA، CAT و SOD پس از استخراج بافت مغز اندازه‌گیری شدند. نتایج: در گروه¬های مداخله مقادیر گیرنده لپتین به طور معنی¬داری بالاتر (001/0=P)، وزن چربی احشایی و BMI کمتر از گروه کنترل سالم بود (001/0=P). پس از هشت هفته تمرین هوازی و مصرف مکمل ویتامین C، مقادیر MDA به میزان معنی‌داری در گروه تمرین+مکمل کاهش پیدا کرد و مقادیر CAT و SOD افزایش پیدا کرد (05/0>P). نتیجه گیری: به نظر می‌رسد تمرین هوازی همراه با مکمل ویتامین C موجب افزایش بیان گیرنده لپتین، کاهش وزن چربی احشایی و BMI در موش های صحرایی مبتلا به بیماری آلزایمر می گردد و همچنین مقادیر MDA را کاهش و مقادیر CAT و SOD را افزایش می دهد.
Background & Aim: Antioxidant enzymes serve as the primary defense against the onslaught of various reactive oxygen radicals. Antioxidants mitigate or prevent oxidative stress by interacting with free radicals and active species. The present study aimed to determine the effect of a course of aerobic exercise comined with vitamin C supplementation on leptin receptor, visceral fat weight, body mass index (BMI), and antioxidant enzymes in the brain tissue of rats with Alzheimer's. Materials & Methods: In this experimental study, 45 elderly female rats were randomly assigned to six groups, including controls with Alzheimer's disease, vitamin C supplementation, aerobic exercise, aerobic exercise + vitamin C, and healthy controls. Leptin, visceral fat weight, and BMI after the intervention, as well as malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) indices, were measured after heart tissue extraction. Results: In the intervention groups, leptin receptor values were significantly higher (P=0.001), while visceral fat weight and BMI were lower than in the healthy control group (P=0.001). After eight weeks of aerobic exercise and vitamin C supplementation, MDA levels decreased significantly in the exercise+supplement group (P<0.05) and values of CAT and SOD were significantly increased compared to other groups (P<0.05). Conclusion: It seems that aerobic exercise combined with vitamin C supplements increases leptin receptor expression and decreases visceral fat weight and BMI in rats with Alzheimer's disease. Moreover, it decreases MDA levels and increases CAT and SOD levels.
1. Pini L, Pievani M, Bocchetta M, Altomare D, Bosco P, Cavedo E, et al. Brain atrophy in Alzheimer’s disease and aging. Ageing research reviews. 2016;30:25-48.
2. Niu H, Álvarez-Álvarez I, Guillén-Grima F, Aguinaga-Ontoso I. Prevalence and incidence of Alzheimer's disease in Europe: A meta-analysis. Neurología (English Edition). 2017;32(8):523-32.
3. McGill-Carter T. Market analysis Alzheimer’s disease 2020. J Psychiatry. 2020;22(6):21-2.
4. Calvo‐Ochoa E, Arias C. Cellular and metabolic alterations in the hippocampus caused by insulin signalling dysfunction and its association with cognitive impairment during aging and Alzheimer's disease: studies in animal models. Diabetes/metabolism research and reviews. 2015;31(1):1-13.
5. Emmerzaal TL, Kiliaan AJ, Gustafson DR. 2003-2013: a decade of body mass index, Alzheimer's disease, and dementia. Journal of Alzheimer's Disease. 2015;43(3):739-55.
6. Chen WW, Zhang X, Huang WJ. Role of physical exercise in Alzheimer's disease. Biomedical reports. 2016;4(4):403-7.
7. Powers SK, Goldstein E, Schrager M, Ji LL. Exercise training and skeletal muscle antioxidant enzymes: An update. Antioxidants. 2022;12(1):39.
8. Zabel M, Nackenoff A, Kirsch WM, Harrison FE, Perry G, Schrag M. Markers of oxidative damage to lipids, nucleic acids and proteins and antioxidant enzymes activities in Alzheimer's disease brain: a meta-analysis in human pathological specimens. Free Radical Biology and Medicine. 2018;115:351-60.
9. Tsukiyama Y, Ito T, Nagaoka K, Eguchi E, Ogino K. Effects of exercise training on nitric oxide, blood pressure and antioxidant enzymes. Journal of clinical biochemistry and nutrition. 2017;60(3):180-6.
10. Pehlivan FE. Vitamin C: An antioxidant agent. Vitamin C. 2017;2:23-35.
11. Zhou T, Prather ER, Garrison DE, Zuo L. Interplay between ROS and antioxidants during ischemia-reperfusion injuries in cardiac and skeletal muscle. International journal of molecular sciences. 2018;19(2):417.
12. Ammar A, Trabelsi K, Boukhris O, Glenn JM, Bott N, Masmoudi L, et al. Effects of aerobic-, anaerobic-and combined-based exercises on plasma oxidative stress biomarkers in healthy untrained young adults. International Journal of Environmental Research and Public Health. 2020;17(7):2601.
13. Lu K, Wang L, Wang C, Yang Y, Hu D, Ding R. Effects of high-intensity interval versus continuous moderate‑intensity aerobic exercise on apoptosis, oxidative stress and metabolism of the infarcted myocardium in a rat model. Molecular medicine reports. 2015;12(2):2374-82.
14. Bouzid MA, Hammouda O, Matran R, Robin S, Fabre C. Influence of physical fitness on antioxidant activity and malondialdehyde level in healthy older adults. Applied Physiology, Nutrition, and Metabolism. 2015;40(6):582-9.
15. Cermakova P, Eriksdotter M, Lund L, Winblad B, Religa P, Religa D. Heart failure and Alzheimer′ s disease. Journal of internal medicine. 2015;277(4):406-25.
16. Spanos F, Liddelow SA. An overview of astrocyte responses in genetically induced Alzheimer’s disease mouse models. Cells. 2020;9(11):2415.
17. Nobakht M, Hoseini SM, Mortazavi P, Sohrabi I, Esmailzade B, Roosh NR, et al. Neuropathological changes in brain cortex and hippocampus in a rat model of Alzheimer’s disease. Iranian biomedical journal. 2011;15(1-2):51.
18. Eskandari RE, Homaee HM, Moradi L. Effect of Eight Weeks Aerobic Exercise and Vitamin-D Supplementation on Osteocalcin and Alkaline Phosphatase Gene Expression in Male Rats Poisoned with Hydrogen Peroxide. Journal of Shahid Sadoughi University of Medical Sciences. 2021.
19. ElBakry RH, Tawfik SM. Histological study of the effect of potassium dichromate on the thyroid follicular cells of adult male albino rat and the possible protective role of ascorbic acid (vitamin C). Journal of Microscopy and Ultrastructure. 2014;2(3):137-50.
20. Uysal N, Agilkaya S, Sisman AR, Camsari UM, Gencoglu C, Dayi A, et al. Exercise increases leptin levels correlated with IGF-1 in hippocampus and prefrontal cortex of adolescent male and female rats. Journal of chemical neuroanatomy. 2017;81:27-33.
21. Polak J, Klimcakova E, Moro C, Viguerie N, Berlan M, Hejnova J, et al. Effect of aerobic training on plasma levels and subcutaneous abdominal adipose tissue gene expression of adiponectin, leptin, interleukin 6, and tumor necrosis factor α in obese women. Metabolism. 2006;55(10):1375-81.
22. Khorshidi D, Azizbeigi K, Abedi B. Effect of progressive aerobic training on leptin, insulin, cortisol and testosterone in obese sedentary men. Scientific Journal of Kurdistan University of Medical Sciences. 2014;19(4):118-27.
23. Akbarpour M. The effect of aerobic training on serum adiponectin and leptin levels and inflammatory markers of coronary heart disease in obese men. Biology of sport. 2013;30(1):21-7.
24. Saghebjoo M, Fathi R, TALEBI GE, HOSSEINI KA, GHANBARI NA, Hedayati M. Obestatin and the regulation of energy balance in physical activity. 2011.
25. Lichtenstein MB, Andries A, Hansen S, Frystyk J, Støving RK. Exercise addiction in men is associated with lower fat-adjusted leptin levels. Clinical Journal of Sport Medicine. 2015;25(2):138-43.
26. Camarda SRdA, Tebexreni AS, Páfaro CN, Sasai FB, Tambeiro VL, Juliano Y, et al. Comparison of maximal heart rate using the prediction equations proposed by Karvonen and Tanaka. Arquivos brasileiros de cardiologia. 2008;91:311-4.
27. Ranji P, Agah S, Heydari Z, Rahmati-Yamchi M, Alizadeh AM. Effects of Lactobacillus acidophilus and Bifidobacterium bifidum probiotics on the serum biochemical parameters, and the vitamin D and leptin receptor genes on mice colon cancer. Iranian journal of basic medical sciences. 2019;22(6):631.
28. Moosavirad SA, Rabbani M, Sharifzadeh M, Hosseini-Sharifabad A. Protective effect of vitamin C, vitamin B12 and omega-3 on lead-induced memory impairment in rat. Research in Pharmaceutical Sciences. 2016;11(5):390.
29. Zhang X-Y, Xu Z-P, Wang W, Cao J-B, Fu Q, Zhao W-X, et al. Vitamin C alleviates LPS-induced cognitive impairment in mice by suppressing neuroinflammation and oxidative stress. International immunopharmacology. 2018;65:438-47.
30. Fatemi Tabatabaei SR, Shahriari A, Abaszadeh M. Effect of vitamin c on the obesity indices of high fat fed diet rats. Jundishapur Scientific Medical Journal. 2014;13(1):32-42.
31. Garcia-Diaz D, Campion J, Milagro F, Boque N, Moreno-Aliaga MJ, Martinez JA. Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes. Journal of Molecular Endocrinology. 2010;45(1):33-43.
32. Ide K, Yamada H, Umegaki K, Mizuno K, Kawakami N, Hagiwara Y, et al. Lymphocyte vitamin C levels as potential biomarker for progression of Parkinson's disease. Nutrition. 2015;31(2):406-8.
33. Monacelli F, Acquarone E, Giannotti C, Borghi R, Nencioni A. Vitamin C, aging and Alzheimer’s disease. Nutrients. 2017;9(7):670.
34. Jolitha A, Subramanyam M, Devi SA. Modification by vitamin E and exercise of oxidative stress in regions of aging rat brain: studies on superoxide dismutase isoenzymes and protein oxidation status. Experimental gerontology. 2006;41(8):753-63.
35. Cobley JN, McHardy H, Morton JP, Nikolaidis MG, Close GL. Influence of vitamin C and vitamin E on redox signaling: Implications for exercise adaptations. Free Radical Biology and Medicine. 2015;84:65-76.
36. Yan Z, Spaulding HR. Extracellular superoxide dismutase, a molecular transducer of health benefits of exercise. Redox biology. 2020;32:101508.
37. Hollander J, Fiebig R, Gore M, Ookawara T, Ohno H, Ji L. Superoxide dismutase gene expression is activated by a single bout of exercise in rat skeletal muscle. Pflügers Archiv. 2001;442:426-34.
38. Okutsu M, Call JA, Lira VA, Zhang M, Donet JA, French BA, et al. Extracellular superoxide dismutase ameliorates skeletal muscle abnormalities, cachexia, and exercise intolerance in mice with congestive heart failure. Circulation: Heart Failure. 2014;7(3):519-30.
39. Radák Z, Asano K, Inoue M, Kizaki T, Oh-Ishi S, Suzuki K, et al. Superoxide dismutase derivative prevents oxidative damage in liver and kidney of rats induced by exhausting exercise. European journal of applied physiology and occupational physiology. 1996;72:189-94.
40. Tuon T, Valvassori SS, Lopes-Borges J, Luciano T, Trom CB, Silva LA, et al. Physical training exerts neuroprotective effects in the regulation of neurochemical factors in an animal model of Parkinson's disease. Neuroscience. 2012;227:305-12.