ارزیابی تنوع ژنتیکی برخی جمعیت‌های هندی نماتد سیستی لوبیای سودانی Heterodera cajani با استفاده از RAPD
محورهای موضوعی : گیاه پزشکیمحمد عبدالهی 1 , آجی کومار گانگولی 2
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کلید واژه: تنوع ژنتیکی, Genetic variation, RAPD, India, لوبیای سودانی, Heterodera cajani, هندوستان, Pigeonpea, Cyst nematode,
چکیده مقاله :
یکی از مهم‌ترین عوامل خسارت‌زای لوبیای سودانی، نماتد سیستی Heterodera cajani Koshy است که در هندوستان خسارت اقتصادی قابل توجهی به این محصول و برخی حبوبات دیگر وارد می‌کند. اطلاع از تنوع ژنتیکی جمعیت‌های این عامل خسارت‌زا در مناطق جغرافیایی مختلف، به انتخاب استراتژی‌های مؤثر در کنترل آن کمک بسیاری خواهد کرد. در تحقیق حاضر، با استفاده از روش RAPD، تنوع ژنتیکی ده جمعیت از نماتد H. cajani به دست آمده از مناطق مختلف تحت کشت لوبیای سودانی ارزیابی گردید. در این مطالعه از پرایمرهای ده تایی به عنوان سری‌های آغازکننده برای بررسی چندشکلی ملکول DNA استفاده گردید. تعداد قطعات تکثیر یافته به ازای هر پرایمر از 7 (در مورد OPA 16 و OPD 4) تا 24 (در مورد OPB 2) متغیر بود در حالی که اندازه‌ها بین 300 جفت باز تا 1/3 کیلو باز تغییر نشان دادند. از 32 پرایمر مورد آزمایش، 23تای آنها (OPA 1, 2, 3, 4, 5, 6, 10 و OPB 2, 3, 4, 12 و OPC 1, 4, 5, 7, 8, 9, 10, 11, 16 و OPD 4, 10) 100 درصد چندشکلی از خود نشان دادند. در مجموع 451 قطعه تکثیر گردید که از این تعداد 434 چندشکلی و 17 تک‌شکلی بودند. ضریب جاکارد و تجزیه خوشه‌ای داده‌های به دست آمده نشان داد که جمعیت به دست آمده از منطقه ایندور از جمعیت سایر مناطق متمایز است. جمعیت‌های به دست آمده از منطقه سمستیپور و ایستگاه منطقه‌ای پوسا بیشترین خویشاوندی را دارا بودند.
One of the most important pathogen attacking pigeonpea root, the nematode Heterodera cajani Koshy, is causing significant economic losses in India. Knowledge of genetic variability present among different geographical populations is important for the selection of suitable control strategies. Molecular diversity among ten populations of H. cajani in India from pigeonpea growing areas is demonstrated by random amplified polymorphic DNA (RAPD). Operon series of decamer primers were used for exploring the polymorphism. The number of amplified fragments per primer varied from 7 (with OPA 16 and OPD 4) to 24 (with OPB 2) whereas the size varied from 300 bp to 3.1 kb. Out of the 32 primers tested 23 revealed 100% polymorphism (OPA 1, 2, 3, 4, 5, 6, 10, OPB 2, 3, 4, 12, OPC 1, 4, 5, 7, 8, 9, 10, 11, 16, OPD 4 and 10). Overall, 451 amplification products were obtained out of which, 434 were polymorphic and 17 were monomorphic. The Jaccard’s coefficient and cluster analysis showed that the nematode population from Indore was distinct from the other populations of H. cajani in India. Samastipur and Pusa R.S. populations showed high degree of similarity in this experiment.
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