Evaluation of genetic variation among Indian populations of the pigeonpea cyst nematode, Heterodera cajani, by RAPD

Subject Areas : Plant Protection

Mohammad Abdollahi ¹ , Ajoy Kumar Ganguly ²

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Received: 2011-01-18 Accepted : 2011-01-18 Published : 2009-12-22

Keywords:

Abstract :

One of the most important pathogen attacking pigeonpea root, the nematode Heterodera cajani Koshy, is causing significant economic losses in India. Knowledge of genetic variability present among different geographical populations is important for the selection of suitable control strategies. Molecular diversity among ten populations of H. cajani in India from pigeonpea growing areas is demonstrated by random amplified polymorphic DNA (RAPD). Operon series of decamer primers were used for exploring the polymorphism. The number of amplified fragments per primer varied from 7 (with OPA 16 and OPD 4) to 24 (with OPB 2) whereas the size varied from 300 bp to 3.1 kb. Out of the 32 primers tested 23 revealed 100% polymorphism (OPA 1, 2, 3, 4, 5, 6, 10, OPB 2, 3, 4, 12, OPC 1, 4, 5, 7, 8, 9, 10, 11, 16, OPD 4 and 10). Overall, 451 amplification products were obtained out of which, 434 were polymorphic and 17 were monomorphic. The Jaccard’s coefficient and cluster analysis showed that the nematode population from Indore was distinct from the other populations of H. cajani in India. Samastipur and Pusa R.S. populations showed high degree of similarity in this experiment.

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