Biochemical and Physiological Responses of Tuberose (Polianthes tuberosa L.) Cut Flower to Silver Nanoparticles Treatment
محورهای موضوعی : مجله گیاهان زینتی
Keramatallah Mohammadi
1
,
Saeid Chavoshi
2
,
Hossein Ali Asadi-Gharneh
3
,
Masoud Gomarian
4
,
Behzad Edrisi
5
1 - Department of Horticultural Sciences, Arak Branch, Islamic Azad University, Arak, Iran
2 - Department of Agronomy and Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran
3 - Department of Horticulture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
4 - Department of Agronomy and Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran
5 - Department of Horticultural Sciences, Arak Branch, Islamic Azad University, Arak, Iran|Ornamental Plants Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mahallat, Iran
کلید واژه: postharvest, Vase life, Sucrose, Antioxidant, nano-silver, <i>Polianthes tuberosa</i>,
چکیده مقاله :
Tuberose is an valuable ornamental plant with a high economic value, which is among the five leading cut flowers of the world. The major obstacle hindering the development of cut flower exports, especially tuberoses, is their short postharvest vase life caused by the disruption of the plant’s water relations due to growth and an increase in vase solution microbial load. The quality and longevity of flowers are highly dependent on their stem stability and antioxidant systems. This research investigated the effect of Nano-silver (NS) applied to the cut flowers of tuberoses cv. ‘Dezfuli’ in 24 h pulse treatments in a randomized complete design. The cut flowers were treated with NS at four rates (0 as control, 10, 20, and 30 mg/L) along with 3% sucrose. Then, the treated flowers were fully immersed in deionized water. The results showed that the application of NS treatment positively increased the activity of antioxidant enzymes and improved postharvest conditions in Polianthes tuberosa L. (cut tuberose flower). Moreover, NS reduced the accumulation of malondialdehyde (MDA) in flower stems and also had a positive effect on the rate of chlorophyll increase compared to the control. NS extended the postharvest vase life of the treated flowers versus the control and increased solution uptake and fresh weight of the cut flowers. In this experiment, the 10 mg/L rate of NS exhibited the best results.
گل مریم از با ارزش‌ترین گیاهان زینتی و جزو پنج گل برتر شاخه بریده جهان است که دارای ارزش اقتصادی بالایی میباشد. از مهمترین مشکلات توسعه صادرات گلهای شاخه بریده بویژه مریم به دلیل عمر کم پس از برداشت و به دلیل به هم خوردن روابط آبی گیاه در اثر رشد و افزایش جمعیت میکروبی در محلول گلجایی میباشد. در کشاورزی مدرن در سالهای اخیر از نانو ذرات به خاطر خواص ضد میکروبی در صنعت پس از برداشت در محصولات کشاورزی استفاده میشود. کیفیت و ماندگاری گلها تا حدود زیادی به پایداری ساقه و سیستم آنتیاکسیدانی بستگی دارد. در این پژوهش اثرات نانو نقره بر روی گلهای شاخه بریده مریم رقم ’دزفولی‘ در قالب طرح کاملاً تصادفی به‌صورت تیمار پالسی (24 ساعت) انجام شد. گلهای شاخه بریده در چهار غلظت نانو ذرات نقره (0، 10، 20 و 30 میلیگرم در لیتر) به همراه ساکارز 3 درصد تیمار شدند و سپس گلهای تیمار شده تا انتها در آب دیونیزه شده قرارداده شدند. نتایج نشان داد بکارگیری تیمار نانو ذرات نقره باعث افزایش مثبت فعالیت خصوصیات فیزیولوژیکی و بهبود شرایط پس از برداشت در گلهای شاخه بریده مریم شد. نانو ذرات نقره همچنین باعث کاهش تجمع مالوندیآلدئید در ساقههای گل گردید و موجب تاثیر مثبت بر میزان افزایش کلروفیل نسبت به شاهد شد. بکارگیری نانو نقره منجر به افزایش عمر پس از برداشت گلهای تیمار شده نسبت به شاهد گردیده و باعث افزایش جذب محلول، وزن‌تر و شکوفایی در گلهای شاخه بریده شد. نتایج این تحقیق دلالت بر آن دارد نانو نقره بهعنوان یک تنظیم کننده رشد در پس از برداشت میباشد. در این آزمایش غلظت 10 میلیگرم در لیتر بهترین نتیجه را به همراه داشت.
Aarts, J.F.T. 1957. Over de houdbaarheid van snijbloemen. Meded de Landbouw hogeschool te Wageninge, 57: 1-62.
Abeles, F.B. and Biles, C.L. 1991. Characterization of peroxidase in lignifying peach fruit endocarp. Plant Physiology, 95: 269-273.
Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24: 1-15.
Baker, J.E., Lalang, C.Y., Lieberman, M. and Hardonberg, R. 1977. Delay of senescence in carnation by a rhizobitoxine analog and sodium benzoate. Horticultural Science, 12: 28–39.
Boonyanitlpony, P., Kumar, P., Kositsup, B. and Baruah, S. 2011. Effect of zinc oxide nanoparticles on roots of rice (Oryza sativa L.). World Journal of Environmental Biosciences, 27: 172-176.
da Silva, L.C., Oliva, M.A., Azevedo, A.A. and De Araujo, J.M. 2006. Responses of restinga plant species to pollution from an iron pelletization factory. Water, Air, and Soil Pollution, 175(1): 241-256.
Elgimabi, M.N. and Ahmed, O.K. 2009. Effects of bactericides and sucrose-pulsing on vase life of rose cut flowers (Rosa hybrida). Botany Research International, 2 (3): 164-168.
Feng, Q.L., Wu, J., Chen, G.Q., Cui, F.Z., Kim. T.N. and Kim, J.O. 2000. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. Journal of Biomedical Materials Research, 52: 662-668.
Gerailoo, S. and Ghasemnezhad, M. 2011. Effect of salicylic acid on antioxidant enzyme activity and petal senescence in ‘Yellow Island’ cut rose flowers. Journal of Fruit and Ornamental Plant Research, 19: 183-193.
Goldwasser, Y., Herschhorn, J., Plakhine, D., Klefeld, Y. and Rubin, B. 1999. Biochemical factors involved in vetch resistance to Orobanche aegyptiaca. Physiological and Molecular Plant Pathology, 54: 87-96.
Hafis, C., Romero-Puertas, M.C. and Sandalio, L.M. 2011. Antioxidative response of Hordeum maritimum L. to potassium deficiency. Acta Physiologiae Plantarum, 33: 193–202.
Halevy, A.H. and Mayak, S. 1979. Senescence and postharvest physiology of cut flowers. Part 1. Horticultural Reviews, 1: 204-236.
Hatami, M. and Ghorbanpour, M. 2014. Defense enzyme activities and biochemical variations of Pelargonium zonale in response to nanosilver application and dark storage. Turkish Journal of Biology, 38: 130-139.
Heath, R. and Packer, L. 1969. Photoperoxidation in isolated chloroplast, kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemmistry, 125: 189-198.
Hettarachchi, M.P. and Balas, J. 2005. Postharvest handling of cut kniphofia (Kniohofia uvaris oken ‘Flamenco’) flowers. Acta Horticulturae, 669: 359-365.
Hoque, T.S., Hossain, M.A., Mostofa, M.G., Burritt, D.J., Fujita, M. and Tran, L.S.P. 2016. Methylglyoxal: An emerging signaling molecule in plant abiotic stress responses and tolerance. Frontiers in Plant Science, 7 (Article 1341): 1-11.
Hossain, M.A., Bhattacharjee, S., Armin, S.M., Qian, P., Xin, W., Li, H.Y., Burritt, D.J., Fujita, M. and Tran, L.S.P. 2015. Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: Insights from ROS detoxification and scavenging. Frontiers in Plant Science, 6: 420. https://doi.org/10.3389/fpls.2015.00420
Hung, K.T. and Kao, C.H. 2005. Nitric oxide counteracts the senescence of rice leaves induced by hydrogen peroxide. Botanical Bulletin- Academia Sinica Taipei, 46: 21–28.
Ichimura, K. 1998. Improvement of postharvest life in several cut flowers by the addition of sucrose. Japan Agriculture Reserch Quartery, 32: 275-280.
Ichimura, K. and Goto, R. 2002. Effect of gibberellin A3 on leaf yellowing and vase life of cut Narcissus tazzeta var Chinensis flowers. Journal of Japanese Society of Horticulturae, 69 (4): 423–427.
Ichimura, K., Yoshioka, S. and Yumoto-Shimizu, H. 2008. Effects of silver thiosulfate complex (STS), sucrose and combined pulse treatments on the vase life of cut snapdragon flowers. Environmental Control in Biology, 46: 155-162.
Knee, M. 2000. Selection of biocides for use in floral preservatives. Postharvest Biology and Technology, 18: 227–234.
Kochert, G. 1978. Carbohydrate determination by the phenol sulfuric acid method, in Helebust. In: Handbook physiological methods. (Ed. Craig, J. S.) 96-97. Cambridge University Press, Cambridge.
Kumar, N., Pal, M., Singh, A., Sairam, R.K. and Srivastava, G.C. 2010. Exogenous proline alleviates oxidative stress and increase vase life in rose. Scientia Horticulturae, 127: 79-85.
Li, H.B., Li, H.M., Liu, J., Luo, Z., Joyce, D.C. and He, S. 2017. Nano-silver treatments reduced bacterial colonization and biofilm formation at the stem-ends of cut gladiolus ‘Eerde’ spikes. Postharvest Biology and Technology, 123: 102–111. Https: //doi.org/10.1016/j. postharvbio.2016.08.014.
Liu, R. and Lal, R. 2015. Potentials of engineered nanoparticles as fertilizers for increasing agronomic productions. Science of the Total Environment, 514: 131–139.
Liu, J., He, S., Zhang, Z., Cao, J., Lv, P., He, S., Chenge, G. and Joyce, D.C. 2009a. Nano-silver pulse treatments inhibit stem-end bacteria on cut gerbera cv. Ruikou flowers. Postharvest Biology and Technology, 54: 59-62.
Liu, J., Zhang, Z., He, S., Cao, J., Lv, P. and Joyce, D.C. 2009b. Effects of postharvest nano-silver treatments on cut flowers. International Society for Horticultural Science (ISHS) Postharvest Quality of Ornamentals conference, Odense, Denmark, August 11–14th.
Lu, P., Cao, J., He, Sh., Liu, J., Li, H., Chenga, G., Dinga, Y. and Joycec, D.C. 2010. Nano-silver pulse treatments improve water relations of cut rose cv. Movie Star flowers. Postharvest Biology and Technology, 57: 196–202.
Madadzadeh, N., Hassanpour Asil, M. and Roein, Z. 2014. Effect of essential oils and silver nanoparticles (SNP) on vase life of Alstroemeria cut flowers (cv. Sukari). Iranian Journal of Horticultural Science, 45: 65-78. (In Persian)
Mayak, S., Kofranek, A.M. and Tirosh, T. 1978. The effect of inorganic salts on geophytes plants. Acta Horticulturae, 325: 131–137.
Naidu, S.N. and Reid, M.S. 1989. Postharvest handling of tuberose (Polianthes tuberosa L.). Acta Horticulturae, 261: 313–318.
Naing, A.H. and Kim, C.K. 2020. Application of nano-silver particles to control the postharvest biology of cut flowers: A review. Scientia Horticulturae, 270: 109463. https://doi.org/10.1016/j.scienta.2020.109463
Naing, A.H., Win, N.M., Han, J.S., Lim, K.B. and Kim, C.K., 2017. Role of nano-silver and the bacterial strain Enterobacter cloacae in increasing vase life of cut carnation ‘Omea’. Frontiers in Plant Science, 8: 1590. https://doi.org/10.3389/fpls. 2017.01590.
Nalousi, M., Hatamzadeh, A., Azadi, P., Mohsenpour, M. and mizadeh, L.H. 2019. A procedure for indirect shoot organogenesis of Polianthes tuberosa L. and analysis of genetic stability using ISSR markers in regenerated plants. Scientia Horticulturae, 244: 315-321.
Priyadarshini, S., Deepesh, B., Zaidi, M.G.H., Pardha Saradhi, P., Khanna, P.K. and Arora, S. 2012. Silver nanoparticle-mediated enhancement in growth and antioxidant status of Brassica juncea. Applied Biochemistry and Biotechnology, 167: 2225–2233.
Rabiza-Świder, J., Skutnik, E., Jędrzejuk, A. and Rochala Wojciechowska, J. 2020. Nanosilver and sucrose delay the senescence of cut snapdragon flowers. Postharvest Biology and Technology, 165: 111165. https://doi.org/10.1016/ j.postharvbio.2020.111165
Roy, S., Barua, N., Buragohain, A.K. and Ahmed, G.A. 2013. Study of ZnO nanoparticles: Antibacterial property and light depolarization property using light scattering tool. Journal of Quantitative Spectroscopy and Radiative Transfer, 118: 8-13.
Sastry, R., Rao, N. and Richard, C. 2010. Can nanotechnology provide the innovations for a second green revolution in India agriculture? Biological Trace Element Research, 13: 639-648.
Setyadjit, S., Joyce, D.C., Irving, D.E. and Simons, D.H. 2004. Effects of 6-benzylaminopurine treatment on the longevity of harvested grevillea ‘Sylvia’ inflorescences. Journal of Plant Growth Regulation, 43: 9-14.
Sikora, M. and Świeca, M. 2018. Effect of ascorbic acid postharvest treatment on enzymatic browning, phenolics and antioxidant capacity of stored mung bean sprouts. Food Chemistry, 239: 1160–1166.
Soleimani Aghdam, M., Naderi, R., Sarcheshmeh, M.A.A. and Babalar, M. 2015. Amelioration of postharvest chilling injury in anthurium cut flowers by γ-aminobutyricacid (GABA) treatments. Postharvest Biology and Technology, 110: 70–76.
Solgi, M., Kafi, M., Taghavi, T.S. and Naderi, R. 2009. Essential oils and silver nanoparticles (SNP) as novel agents to extend vase life of gerbera (Gerbera jamesonii cv. ‘Dune’) flowers. Postharvest Biology and Technology, 53: 155–158.
Waithaka, K., Reid, M.S. and Dodge, L.L. 2001. Cold storage and power keeping quality of cut tuberose (Polianthes tuberosa L.). Journal of Horticultural Science and Biotechnology, 76 (3): 271-275.
Yamada, K., Ito, M., Oyama, T. and Nakada, M. 2007. Analysis of sucrose metabolism during petal growth of cut roses. Postharvest Biology and Technology, 43: 175-177.
Zhao, D., Cheng, M., Tang, W., Liu, D., Zhou, S., Meng, J. and Tao, J. 2018. Nano-silver modifies the vase life of cut herbaceous peony (Paeonia lactiflora Pall.) flowers. Protoplasma, 1: 1–13.