Effect of Seasons, Gender and Agrobacterium rhizogenes Strains on Adventitious Root Induction of Male and Female Juniperus communis L.

محورهای موضوعی : مجله گیاهان زینتی

Mostafa Khoshhal Sarmast ¹ (Department of Horticultural Science, Faculty of Plant Production, Gorgan University, Faculty of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran)
Rudabeh Kordkatoli ² (Department of Horticultural Science, Faculty of Plant Production, Gorgan University, Faculty of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran)
Zahra Rezaei ³ (Department of Horticultural Science, Faculty of Plant Production, Gorgan University, Faculty of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran)
Azim Ghasemnezhad ⁴ (Department of Horticultural Science, Faculty of Plant Production, Gorgan University, Faculty of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran)

تاریخ دریافت : 1397/07/10 تاریخ پذیرش : 1397/11/09 تاریخ انتشار : 1397/12/10

کلید واژه: Season, Adventitious rooting, Common juniper, Dioecy, <i>Agrobacterium rhizogenes</i>,

چکیده مقاله :

Scrutinizing of different aspects in vegetative propagation of Iranian J. communis L. is of prime importance in order to prevent extinction of such valuable conifer. In this research, the effects of different seasons, gender, different chemical compounds (IBA, PBZ, putrescine and sodium nitroprusside) and three strains of Agrobacterium rhizogenes on ex-vitro rooting capacity of male and female J. communis L. were evaluated. Rooting of semi-hard wood J. communis L.cuttings in autumn were notably successful either in female or male plants. Research results indicated that cutting gained in September provides the highest rooting percentage in female plants while no significant differences in rooting ability were found in male plants which were collected in December and September. There were a female-biased root induction trends between genders. Treatment of female cuttings with IBA declined rooting to the half, even in male cuttings it completely suppress root induction ability compared to control plants. Our empirical evidence on presence of genetic difference in random amplified loci of male and female plants to some extent can explain the difference in rooting competency which can be influenced by difference in genetic make up. A4 strain of A. rhizogenes managed to yield 53.5% rooting compared to the other strains in male plants. The gender-mediated adventitious root formation of J. communis L. which has supported with some genetic evidence will pave the way for further fundamental studies to delineate the missing molecular key of rooting mechanisms.

چکیده انگلیسی:

موشکافی جنبه&shy;های مختلف افزایش رویشی سرو کوهی ایرانی یکی از مهمترین اولویت&shy;ها در رابطه با جلوگیری از انقراض این گونه&shy;ها به حساب می&shy;آید. در این پژوهش اثر سویه&shy;های مختلف اگروباکتریوم رایزوژنز، ترکیبات هومونی مختلف (ایندول بوتریک اسید، پاکلوبوترازول، پوتریسین و سدیم نیتروپروساید)، فصول مختلف و جنسیت&shy;های متفاوت در توانایی ریشه&shy;زایی دو پایه نر و ماده سرو کوهی مورد بررسی قرار گرفت. ریشه&shy;زایی قلمه&shy;های نیمه خشبی سرو کوهی در هر دو پایه در پاییز موفقیت&shy;آمیزتر بود. قلمه&shy;های گرفته شده در سپتامبر بالاترین درصد ریشه&shy;زایی را در پایه ماده داشتند در حالی که تفاوت معنی&shy;داری در توانایی ریشه&shy;زایی در پایه&shy;های نر که در دسامبر و سپتامبر گرفته شده بود دیده نشد.گرایش به ریشه&shy;زایی در پایه ماده بیشتر از نر بود. تیمار پایه&shy;های ماده با IBA ریشه&shy;زایی را به نصف کاهش داد و حتی این تنظیم کننده رشد ریشه&shy;زایی را در پایه نر نسبت به کنترل به صفر رساند. تفاوت در درصد ریشه&shy;زایی بین پایه&shy;ها تا حدی به&shy;وسیله تفاوت ژنتیکی مشاهده شده در دو پایه با استفاده از تکثیر تصادفی مکان&shy;های ژنی آن&shy;ها قابل توجیه خواهد بود. سویه A4 اگرو باکتریوم رایزوژنز در مقایسه با دیگر سویه&shy;ها ریشه&shy;زایی را در پایه ماده به 5/53 درصد رساند. تشکیل ریشه&shy;های نابجا مرتبط با جنسیت در سرو کوهی که به کمک داده&shy;های ژنتیکی حمایت می&shy;شود راه را برای مطالعات پایه&shy;ای برای آشکار نمودن حلقه گم شده در سازوکار ریشه&shy;زایی گیاهان آشکار خواهد نمود.

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