گیاه Juniperus sabina L.ازجمله گیاهان دارویی ارزشمند شمال ایران است که از اثرات فارماکولوژیکی بسیاری از جمله خواص ضدسرطانی، ضد مالاریا و ضد باروری برخوردار است. پژوهش حاضرباهدف ارزیابی فیتوشیمیایی اسانس و عملکرد آنتی اکسیدانی سرشاخه های گیاه مای مرزدر دو استان گلستان و مازندران انجام شد. نمونه ها از سه رویشگاه رامسر، رامیان وتوسکستان در فصل تابستان سال 1397 جمع آوری شدند. اسانس گیری به روش تقطیر با آب و شناسایی ترکیبات با استفاده از دستگاه GC-FID و GC-MS انجام شد. از دو روش TAOC و DPPH جهت بررسی توانمندی آنتی اکسیدانی و روشرنگ‌سنجی (فولین- سیکالچو) جهت سنجش فنل کل استفاده شد. بیشترین مقدار فنل‌کل در نمونه رامسر (mg GAE/g dw19/0± 33/3)، مهار رادیکال DPPH در نمونه رامیان (99/4 ±1/43 درصد) و آنتی اکسیدان کل در نمونه توسکستان (12/0±05/19 میلی‌گرم اسکوربیک اسید در گرم وزن خشک) مشاهده شد.اگرچه درصد اسانس و توانمندی آنتی اکسیدانی جمعیت‌های مناطق مورد مطالعه تفاوت معنی داری نداشتند، با این وجود تغییرات اجزای اسانس به ویژه ترکیبات قالب کاملاً متفاوت بود. مقدارسابینن به‌عنوان ترکیب غالب در سه رویشگاه مذکور به ترتیب 5/12، 8/49 و 58 درصدبود. نکته قابل توجه در این پژوهش وجود ترکیب میرتنیل-استات به مقدار 6/72 درصد در نمونه رامسر بود که تاکنون در رابطه با این گیاه گزارش نشده است.با توجه به تفاوت بارز مشاهده شده در ترکیب اسیدهای چرب ضروری اسانس مای مرز رامسر، پیشنهاد می‌گردد که این توده گیاهی به دقت بیشتری مورد بررسی قرار گرفته و ممکن است در آینده به عنوان کموتایپی از مای مرز معرفی گردد. پرونده مقاله

Abiotic stress conditions can be imposed on plants through either poor water quality or adverse climate condition. These types of stress affect plant growth and development throughout their life cycle. Plants that are tolerant to drought are therefore valuable, and turf grasses have the potential to grow tolerant. Turf grasses provide ground cover and offer multiple benefits, from their aesthetic value which affects our daily lives to their preservation of land against erosion. The objectives of the present work were to find the ideal media for callus induction and regeneration followed by overexpressing the P5CS1 gene encoding proline for improving drought stress resistance in Festuca arundinacea Schreb. The husks of tall fescue seeds were removed and the seeds were then sliced longitudinally. The result of this treatment showed a greater callus induction efficiency in comparison with intact seeds in the culture media. In addition, an increase in regeneration efficiency was observed in media supplemented with 2,4-D along with BAP. The average of G418‒resistant calli obtained in the experiment was around 10%. The heterologous transformation of P5CS1 in F. arundinacea background was confirmed by PCR and the transient Gus assay. More than 90% of calli expressed the uida gene which can most probably convey resistance to drought stress. پرونده مقاله

Scrutinizing of different aspects in vegetative propagation of Iranian J. communis L. is of prime importance in order to prevent extinction of such valuable conifer. In this research, the effects of different seasons, gender, different chemical compounds (IBA, PBZ, putrescine and sodium nitroprusside) and three strains of Agrobacterium rhizogenes on ex-vitro rooting capacity of male and female J. communis L. were evaluated. Rooting of semi-hard wood J. communis L.cuttings in autumn were notably successful either in female or male plants. Research results indicated that cutting gained in September provides the highest rooting percentage in female plants while no significant differences in rooting ability were found in male plants which were collected in December and September. There were a female-biased root induction trends between genders. Treatment of female cuttings with IBA declined rooting to the half, even in male cuttings it completely suppress root induction ability compared to control plants. Our empirical evidence on presence of genetic difference in random amplified loci of male and female plants to some extent can explain the difference in rooting competency which can be influenced by difference in genetic make up. A4 strain of A. rhizogenes managed to yield 53.5% rooting compared to the other strains in male plants. The gender-mediated adventitious root formation of J. communis L. which has supported with some genetic evidence will pave the way for further fundamental studies to delineate the missing molecular key of rooting mechanisms. پرونده مقاله