پتانسیل ضد کاندیدیايی عصاره دارچین در مدل حیوانی
محورهای موضوعی : قارچ شناسی
فهیمه علیزاده
1
,
مریم رحیمی فرودی
2
,
صادق برازش
3
,
الهام پیشگر
4
,
علیرضا خداوندی
5
1 - گروه زیست شناسی-میکروبیولوژی، واحد یاسوج، دانشگاه آزاد اسلامی، یاسوج، ایران
2 - گروه میکروبیولوژی، دانشکده کشاورزی و علوم نوین، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
3 - گروه زیست شناسی، واحد گچساران، دانشگاه آزاد اسلامی، گچساران، ایران
4 - دانشجوی دکتری، گروه میکروبیولوژی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
5 - استادیار گروه پیراپزشکی دانشگاه گچساران
کلید واژه: کاندیدیازیس, کاندیدا آلبیکانس و مقاومت دارویی, اثر ضد قارچی عصاره دارچین, مدل حیوانی,
چکیده مقاله :
سابقه و هدف: کاندیدیازیس یک عفونت قارچی شایع است که توسط مخمر کاندیدا آلبیکانس ایجاد می‌شود. با افزایش مقاومت به داروهای ضد قارچی، درمان این بیماری با چالش‌های جدی مواجه شده است. بدین ترتیب، امروزه استفاده از ترکیبات گیاهی مانند دارچین به عنوان جایگزین های احتمالی مورد توجه قرار گرفته است. این پژوهش با هدف بررسی پتانسیل ضد قارچی عصاره دارچین در مدل حیوانی انجام شد.
مواد و روش‌ها: در این مطالعه جدایه‌های کاندیدا آلبیکانس و سویه استاندارد PTCC5027 استفاده شدند. عصاره آبی و الکلی دارچین تهیه و اثرات ضد قارچی آن‌ها با روش دیسک گذاری و تعیین MIC و MFC ارزیابی شد. موش‌های آلوده با غلظت‌های مختلف عصاره و فلوکونازول درمان شدند و بار میکروبی در مدفوع و کلیه آن‌ها اندازه گیری شد.
یافته‌ها: عصاره دارچین رشد کاندیدا آلبیکانس را در شرایط برون و درون تنی مهار کرد. همچنین، مقادیرMIC50 وMIC90 عصاره آبی دارچین به ترتیب در محدوده 6.82 - 6.25 میلی‌لیتر بر میلی‌گرم و 12.24 - 11.24 میلی‌لیتر بر میلی‌گرم تعیین شدند، این مقادیر برای عصاره الکلی دارچین به ترتیب در محدوده 6.90 - 6.15 میلی‌لیتر بر میلی‌گرم و 12.42 - 11.07 میلی‌لیتر بر میلی‌گرم بدست آمدند. میزان MFC عصاره‌ها به ترتیب در 13.64- 12.51 میلی‌لیتر بر میلی‌گرم و 13.80 -12.30میلی‌لیتر بر میلی‌گرم محاسبه شد. کاهش معنادار CFU در مدفوع و کلیه موش‌های تیمار شده با عصاره و فلوکونازول مشاهده شد.
نتیجه‌گیری: عصاره دارچین پتانسیل ضد قارچی قابل توجهی در برابر کاندیدا آلبیکانس نشان داد و می‌توانند به‌عنوان جایگزین یا مکمل در درمان کاندیدیازیس مورد استفاده قرار گیرد.
Background and objective: Candidiasis is a common fungal infection caused by Candida albicans. The increasing resistance to conventional antifungal drugs has posed significant challenges in treating this condition. Consequently, plant-derived compounds, such as cinnamon, have gained attention as potential alternatives. This study aimed to investigate the antifungal potential of cinnamon extract in an animal model of candidiasis.
Materials and Methods: Clinical isolates and the standard strain C. albicans PTCC5027 were used. Aqueous and alcoholic extracts of cinnamon were prepared, and their antifungal effects were evaluated through disk diffusion assays, minimum inhibitory concentration (MIC), and minimum fungicidal concentration (MFC). Mice infected with C. albicans were treated with varying concentrations of the extracts and fluconazole. Microbial load in feces and kidney tissues was measured.
Results: Cinnamon extract significantly inhibited the growth of C. albicans under both in vitro and in vivo conditions. The MIC50 and MIC90 values for the aqueous extract were determined to be 6.25–6.82 mg/mL and 11.24–12.24 mg/mL, respectively, while those for the alcoholic extract ranged from 6.15–6.90 mg/mL (MIC50) and 11.07–12.42 mg/mL (MIC90). The MFC values for the aqueous and alcoholic extracts were 12.51–13.64 mg/mL and 12.30–13.80 mg/mL, respectively. A significant reduction in colony-forming units (CFU) was observed in the feces and kidneys of mice treated with the extracts and fluconazole.
Conclusion: Cinnamon extract demonstrated notable antifungal potential against C. albicans and could serve as a complementary or alternative therapeutic agent for candidiasis.
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