به کارگیری سه روش ERIC-PCR, RAPD-PCR, Rep-PCR در دسته بندی ژنتیکی ایزوله های کمپیلوباکتر ژژونی وکمپیلوباکترکولی جدا شده از گوشت خام مرغ
محورهای موضوعی : آلودگی میکروبی مواد غذائی
مریم هادیان
1
,
حسن ممتاز
2
,
امیر شاکریان
3
1 - گروه میکروبیولوژی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
2 - استاد میکروبیولوژی، گروه میکروبیولوژی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
3 - دانشگاه آزاد اسلامی واحد شهرکرد
کلید واژه: کمپیلوباکترژژونی, کمپیلوباکترکلی, ERIC-PCR, RAPD-PCR, Rep-PCR,
چکیده مقاله :
سابقه و هدف: کمپیلوباکترها به ویژه دو گونه کمپیلوباکترژژونی و کمپیلوباکترکولی با داشتن سویه‌ها و میزبان‌های مختلف، یکی از مهم‌ترین و شایع‌ترین باکتری‌های بیماری‌زای مشترک بین انسان و دام محسوب می‌شوند. مطالعه حاضر با هدف دسته بندی ژنتیکی ایزوله های های کمپیلوباکترژژونی و کمپیلوباکترکولی جدا شده از گوشت خام مرغ انجام شد. مواد وروش ها: این مطالعه مقطعی بر روی 36 ایزوله کمپیلوباکترژژونی و کمپیلوباکترکولی جداشده از گوشت خام مرغ انجام گرفت. به منظور ژنوتایپینگ ایزوله ها از سه روش ERIC-PCR, RAPD-PCR, Rep-PCR استفاده گردید. یافته ها: 36 ایزوله کمپیلوباکتر جدا شده از گوشت خام مرغ در روش ERIC-PCR در 7 پروفایل، در روش RAPD-PCR در 3 پروفایل و در روشREP-PCR در 3 پروفایل قرار گرفتند. نتیجه گیری: تمام جدایه های مورد بررسی دارای الگوی باندی پلی مورفیک بودندکه سرعت بالایی از پلی مورفیسم در ژنوم کمپیلوباکترژژونی و کمپیلوباکترکولی را نشان می دهد. روش های مورد استفاده در این مطالعه هر کدام ابزارهای قدرتمند در دسته بندی کمپیلوباکترژژونی و کمپیلوباکترکولی می باشد ولی طبق یافته های حاصله، روش ERIC-PCR روش مناسب تری نسبت به روش های دیگر برای تایپینگ ودسته بندی جدایه های کمپیلوباکترژژونی و کمپیلوباکترکولی می باشد .
Background and aim: Campylobacter, especially the two species Campylobacter jejuni and Campylobacter coli, having different strains and hosts, are considered one of the most important and common pathogenic bacteria between humans and animals. The present study was conducted with the aim of genetic classification of Campylobacter jejuni and Campylobacter coli strains isolated from raw chicken meat. Materials and Methods: This cross-sectional study was conducted on 36 strains of Campylobacter jejuni and Campylobacter coli strains isolated from raw chicken meat. In order to genotyping the isolates, three methods ERIC-PCR, RAPD-PCR, and Rep-PCR were used. Results: 36 Campylobacter strains isolated from raw chicken meat were placed in 7 profiles in ERIC-PCR method, in 3 profiles in RAPD-PCR method and in 3 profiles in REP-PCR method. Conclusion: All investigated strains had a polymorphic band pattern, which shows a high rate of polymorphism in Campylobacter jejuni and Campylobacter coli genomes. The methods used in this study are powerful tools for genotyping of Campylobacter jejuni and Campylobacter coli, but according to the findings, the ERIC-PCR method is a more suitable method than other methods for typing and classifying Campylobacter jejuni and Campylobacter coli strains.
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