The Study of Antioxidant and Cellular Toxicity Effects of Methanol, Ethyl Acetate, Aqueous and n-Hexane Extracts of Symphytum Kurdicum Plant

Subject Areas : Journal of Chemical Health Risks

Shiva Khalil_moghaddam ¹ , Atousa Aliahmadi ² , Nastaran Jalilian ³ , Mohamad Aref Tabad ⁴

1 - Department of Biology,Yadegar - e- Imam Khomeini (RAH) shahr-e-Rey Branch, Islamic Azad University,Tehran,Iran
2 - Department of Biology, Medicinal Plants and Drug Research Institute, Shahid Beheshti University,Tehran,Iran
3 - Forests and Rangelands Research Department, Kermanshah Agricultural and Natural Resources Research and Education Center, (AREEO), Kermanshah,Iran
4 - M. Sc. Graduate of Medicinal plants, Department of Medicinal Plants, ACECR Institute of Higher Education, Kermanshah,Iran

Received: 2020-06-16 Accepted : 2020-09-06 Published : 2021-10-01

Keywords: Antioxidant, Oxidative stress, Cell toxicity, Symphytum kurdicum,

Abstract :

This study was aimed to investigating the antioxidant and cellular toxicity of Symphytum kurdicum. The methanolic extracts of the aerial parts of the plant were prepared through soaking and non-polar to polar cutting of the extract by the liquid-liquid cutting method. The antioxidant effect of the samples was specified by the methods of determining the free radical scanenging 2, 2-diphenyl-1-picrylhydrazyl(DPPH), ferric reducing ability of plasma (FRAP) and the total phenolic content by folin ciocalteu method. Cellular toxicity of the samples on peripheral blood mononuclear cells (PBMC) was performed by 3-(4,5-dimethylthiazol-2-yl) and 2, 5-diphenyltetrazolium bromide (MTT). The results indicated that ethyl acetate and aqueous fractions with IC50 equal to 33.67 and 29.43 μg/ml, respectively, showed the highest ability in DPPH free radicals Scavenging. Moreover, in the study of ferric iron regeneration, the ethyl acetate fraction with a capability of 280.985± 14.007 mM/mg dry weight of sample showed the best regenerative effect against trolox control. The aqueous and ethyl acetate fractions had the highest total phenolic content with 150.765 ± 0.035 and 130.570 ± 0.056 (Gallic acid milligrams/ gram dry weight of sample), respectively. The results of MTT test revealed that all fractions at a concentration much higher than the effective antioxidant concentrations lacked cellular toxicity, too. Given the role of oxidative stress as a predisposing factor in diseases like diabetes, cancer, and cardiovascular disease, aqueous and ethyl acetate fractions are likely to be introduced as pharmacological supplements.

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