African violets Organogenesis Responding to Hormonal Interaction

Subject Areas : Plant Physiology

Maria Missaghi ¹ , Fataneh Yari ² , Amir Mousavi ³ , Younes Mostofi ⁴ , Hamideh Ofoghi ⁵

1 - Department of Horticultural Sciences and Agronomy, Science and Research Branch, Islamic Azad University, P.O. Box 14515/775, Tehran, Iran
2 - Department of Agriculture, Iranian Research Organization for Science and Technology, P.O. Box: 33535111, Tehran, Iran.
3 - Department of Plant Molecular Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, Iran.
4 - Department of Horticultural Sciences, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources. University of Tehran, P.O. Box4111, Karaj, Iran.
5 - Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box 33535111, Tehran, Iran

Received: 2021-02-07 Accepted : 2022-07-03 Published : 2023-04-01

Keywords: tissue culture, regeneration, genotype, PGRs, Saintpaulia ionantha,

Abstract :

In this study, the possibility of organogenesis of leaves and petioles of two African violet cultivars were evaluated using MS media containing various concentration of plant growth regulators (IAA (1,2 mg/l), BA (0.05, 0.1, 0.2, 0.5 mg/l), NAA (1, 2 mg/l), and TDZ (0.01 mg/l)). All experiments were arranged completely randomized design then data analyzed by one-way ANOVA, and means were separated using Duncan’s new multiple range test (𝑃 ≤ 0.01). Direct and indirect organogenesis from the whole leaf with petiole bases were observed in both cultivars. However, the separated leaf blade and petiole were unable to produce any organs. The organogenesis potential of cultivars was significantly different, although the medium containing BA (0.1 mg/l) + IAA (1 mg/l) showed the highest indirect organogenesis (90 %< in both cultivars). In contrast, the media containing BA 0.05 mg/l + TDZ 0.01 mg/l + IAA 2 mg/l and BA 0.05 mg/l + TDZ 0.01 mg/l + IAA 1 mg /l had more success on the induction of direct organogenesis. Therefore, the level of direct and indirect organogenesis appears to be dependent on the interaction between the PGRs and the explant type having a direct correlation with the genotype.

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