Relationship of Lipid Factors in Blood Serum and Seminal Plasma of Afshari Rams

Subject Areas : Camel

A. Pirestani ¹ (Department of Animal Science, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran|Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran|Medicinal Plants Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran)
E. Ziya Motalebipour ² (Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran|Medicinal Plants Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran|Department of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran)

Received: 2022-09-13 Accepted : 2022-09-13 Published : 2022-09-01

Keywords: sperm, blood, Lipid, Seminal plasma, Afshari ram,

Abstract :

The aim of this study was to investigate the relationship of lipid factors of seminal plasma and blood serum in Afshari rams. Four Afshari rams were selected with a mean weight of 50 ± 5 kg and the mean age of 2.5 years. At the same time, blood and semen samples were collected to evaluate total cholesterol, triglycerides, high-density lipoprotein (HDL), and low-density lipoprotein (LDL), while beta-hydroxybutyric acid (BHBA) was measured only in blood samples. After adding semen to the extender, sperm motility, morphology and viability were evaluated by the CASA software, Papanicolaou and Eosin-Nigrosin staining, respectively. The sperm parameters of seasonal and non-seasonal breeding were assessed in pre-freezing and post-thawing. The results of this study showed that cholesterol and HDL levels of ram were significantly higher in seasonal breeding than non-seasonal breeding in both blood and semen samples (P<0.05). In non-seasonal breeding, LDL and triglyceride levels of blood and semen samples were significantly increased respectively (P<0.05). In seasonal breeding, the sperm parameters such as motility, morphology, and viability of rams were significantly higher in pre-freezing. In non-seasonal breeding, total motility was higher significant in post-thawing (P<0.05). Consequently, in the current study, there was a significant correlation between triglyceride of blood and triglyceride of seminal plasma. Also, there was a significant correlation between blood LDL and seminal LDL/HDL ratios for non-seasonal records. Blood LDL/HDL was also significantly correlated with the seminal cholesterol/HDL, which could improve the sperm quality parameters of Afshari ram in the season and non-seasonal breeding.

References:

Aitken R.J. (1990). Motility parameters and fertility. Pp. 285-302 in Control of Sperm Motility: Biological and Clinical Aspects. C. Gagnon, Ed. CRC Press, Boca Raton, Florida.

Am-In N., Kirkwood R.N., Techakumphu M. and Tantasuparuk W. (2011). Lipid profiles of sperm and seminal plasma from boars having normal or low sperm motility. Theriogenology. 75(5), 897-903.

Barbas J.P. and Mascarenhas R.D. (2009). Cryopreservation of domestic animal sperm cells. Cell Tissue Bank. 10(1), 49-62.

Beer-Ljubić B., Aladrović J., Marenjak T.S., Laškaj R., Majić-Balić I. and Milinković-Tur S. (2009). Cholesterol concentration in seminal plasma as a predictive tool for quality semen evaluation. Theriogenology. 72(8), 1132-1140.

Björndahl L., Söderlund I. and Kvist U. (2003). Evaluation of the one step eosin nigrosin staining technique for human sperm vitality assessment. Hum. Reprod. 18(4), 813-816.

Fiser P.S., Ainsworth L. and Fairfull R.W. (1987). Evaluation of a new diluent and different processing procedures for cryopreservation of ram semen. Theriogenology. 28(5), 599-607.

Frenkel G., Peterson R.N., Davis J.E. and Freund M. (1974). Glycerylphosphorylcholine and carnitine in normal human semen and in postvasectomy semen: Differences in concentrations. Fertil. Steril. 25(1), 84-87.

Herdt T.H. (2000). Variability characteristics and test selection in herd-level nutritional and metabolic profile testing. Veterinary clinics of North America, Food Anim. Pract. 16(2), 387-403.

Joshi A., Naqvi S. M.K., Bag S., Dang A.K., Sharma R.C., Rawat P.S. and Mittal J.P. (2003). Sperm motion characteristics of Garole rams raised for a prolonged period in a semi-arid tropical environment. Trop. Anim. Health Prod. 35(3), 249-257.

Kumar D., Joshi A., Naqvi S.M.K., Kumar S., Mishra A.K., Maurya V.P., Arora A.L., Mittal J.P. and Singh V.K. (2007). Sperm motion characteristics of Garole × Malpura sheep evolved in a semi-arid tropical environment through introgression of FecB gene. Anim. Reprod. Sci. 100(1), 51-60.

Liu C.Y., Chou Y.C., Lin S.H., Wu S.T., Cha T.L., Chen H.I. and Tsao C.W. (2017). Serum lipid profiles are associated with semen quality. Asian J. Androl. 19(6), 633-640.

Maldjian A., Pizzi F., Gliozzi T., Cerolini S., Penny P. and Noble R. (2005). Changes in sperm quality and lipid composition during cryopreservation of boar semen. Theriogenology. 63(2), 411-421.

SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc., USA.

World Health Organisation. (1999). WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge University Press, New York.

Yániz J.L., Marco-Aguado M.A., Mateos J.A. and Santolaria P. (2010). Bacterial contamination of ram semen, antibiotic sensitivities, and effects on sperm quality during storage at 15 C. Anim. Reprod. Sci. 122(1), 142-149.