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Manuscript ID : FH-2206-1112 (R1) Visit : 208 Page: 1 - 7

10.30495/fh.2022.68315.1112

Article Type: Original Research

Prevalence, virulence factor and antibiotic susceptibility patterns of Salmonella spp. from poultry products in Ardabil province

Subject Areas :

Ghadir Shahbazi 1 , Jalal Shayegh 2 , Siamak Ghazaei 3 , Mohamad Hossein Movassagh Ghazani 4 , Shahram Hanifian 5

1 - Department of Veterinary Medicine, Faculty of Veterinary and Agriculture, Shabestar Branch, Islamic Azad University, Shabestar, Iran
2 - Department of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
3 - Department of Microbiology, University of Mohaghegh Ardabili, Ardabil, Iran
4 - Department of Veterinary Medicine, Faculty of Veterinary and Agriculture, Shabestar Branch, Islamic Azad University, Shabestar, Iran
5 - Department of Food Science and Technology, Biotechnology Research Center, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Received: 2022-06-29 Accepted : 2022-08-30 Published : 2022-09-01

Keywords: / BOXAIR-PCR, / Drug resistance, / Biofilm, / REP- PCR, <i>Salmonella </i>spp,

Abstract :

Contamination of poultry products by Salmonella spp. is a critical issue in the poultry industry and public health. The present study aimed at molecular detection and typing of Salmonella isolated from poultry products. Moreover, antibiotic resistance patterns and the biofilm formation ability of isolates were determined. Eighty poultry product samples were collected from chicken supply and distribution centers. Salmonella spp. were identified by culture as well as the genus-specific PCR. A slide agglutination test using O grouping polyvalent sera were used for serological identification. BOXAIR and REP-PCR methods were evaluated for the discrimination of Salmonella isolates at the serotype level. Antibiotic susceptibility testing of serotypes against sixteen antibiotics was performed using the standard Kirby-Bauer disc diffusion method. The Microtiter-plate biofilm formation assay measured the extent of biofilm formation. From 80 samples, 11 Salmonella spp. were identified, divided into two serotypes belonging to B and A serogroups. BOX repeat-based PCR (BOXAIR-PCR) and Repetitive element-based PCR (REP-PCR) banding results of isolates revealed 7 and 6 reproducible fingerprint patterns, respectively. The highest resistance was observed in response to ampicillin and doxycycline, followed by chloramphenicol, sulfamethoxazole, trimethoprim, neomycin, and nalidixic acid. Multidrug resistance was detected in all Salmonella serotypes. Seven isolates possessed the ability to produce biofilm with varied adhesion strength. These results revealed the high and unexpected prevalence of Salmonella spp. in poultry products with multiple antibiotic resistance and biofilm production ability. Also, BOXAIR and REP-PCR results revealed high diversity in Salmonella serotypes and subsequently indicated variety in the origin of Salmonella spp.

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