The effect of the alcoholic extract of Metka plant on the expression of AGT, AGTR2 and ATP1A1 genes in kidney cells
Subject Areas : Biotechnological Journal of Environmental Microbiology
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Keywords: Ferula persica, blood pressure, essential oil, phenol, AGT gene, AGTR2 gene, ATP1A1 gene,
Abstract :
Ferula Persica (Metka) is a native plant from the Apiaceae family with numerous medicinal properties, including anti-inflammatory, antipyretic, and blood pressure-lowering effects. This study investigated the antioxidant properties of phenolic extract and essential oils of Metka flowering tops and their effects on the expression of three key blood pressure control genes (AGT, AGTR2, and ATP1A1) in kidney cells (ACHN IBRC C10105).First, the essential oils were extracted by steam distillation and the phenolic extract was extracted with 80% methanol. Evaluation of phenolic compounds by the Folin-Ciocalteu method and DPPH assay showed that the plant has significant antioxidant activity. MTT test at 24, 48 and 72 hours showed a greater effect of essential oils in reducing the viability of cancer cells than phenolic extract. Zemon TAC confirmed that the antioxidant properties of essential oils were superior. Gene expression analysis using Real-Time PCR revealed that both extracts were effective in increasing the expression of AGT, AGTR2, and ATP1A1 genes, but the effect of essential oils was more significant. These results indicate that Metka can be considered a natural option for controlling blood pressure through the regulation of these genes. Given the widespread distribution of the plant in Iran and its fewer side effects than chemical drugs, the development of herbal medicines based on Metka can be effective in managing blood pressure and reducing dependence on synthetic drugs.