تأثیر هشت هفته تمرین هوازی بر ژنوتیپ های AT و TT پلی مورفیسم rs1870377 ژن VEGFR و تغییرات عملکرد هوازی زنان تمرین نکرده

محورهای موضوعی : فصلنامه زیست شناسی جانوری

حدیث رحیمی ¹ (گروه تربیت بدنی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران)
مانیا روزبیانی ² (گروه تربیت بدنی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران)
عباس صارمی ³ (گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه اراک، اراک، ایران)

تاریخ دریافت : 1401/02/23 تاریخ پذیرش : 1401/04/18 تاریخ انتشار : 1401/12/01

کلید واژه: VO2max, چاقی, تمرین هوازی, ژنوتیپ‌ AT, ژنوتیپ TT, VEGFR,

چکیده مقاله :

پژوهش حاضر با هدف بررسی تأثیر هشت هفته تمرین هوازی بر ژنوتیپ های AT و TT پلی مورفیسم rs1870377 ژن VEGFR و تغییرات عملکرد هوازی زنان تمرین نکرده انجام شد. بدین منظور، تعداد 29 زن غیرفعال 30 تا 45 ساله به صورت تصادفی از زنان داوطلب شهرستان شهریار در استان تهران انتخاب شدند. آزمودنی ها 8 هفته تمرین هوازی بصورت 5 جلسه در هفته و هر جلسه 30 دقیقه با شدت 55 تا 75 درصد ضربان قلب ذخیره انجام دادند. در دو هفته اول با 55 تا 65 درصد ضربان قلب بیشینه، در دو هفته دوم با 60 تا 65 درصد ضربان قلب بیشینه و در 4 هفته پایانی با 65 تا 75 درصد ضربان قلب بیشینه به تمرین پرداختند. 10 دقیقه برای گرم کردن و 10 دقیقه برای سرد کردن در هر جلسه تمرینی در نظر گرفته شد. برای تعیین میزان VO2max از آزمون هفت مرحله ای بروس، پیش و پس از تمرینات استفاده شد. آزمون با شیبی معادل 10 درصد و سرعت 7/2 کیلومتر بر دقیقه روی نوار گردان شروع شده و هر مرحله در مدت سه دقیقه انجام شد به طوری که در مرحله هفتم شیب 22 درصد و سرعت 6/9 بود. سپس از بین آزمودنی هایی که توانستند آزمون مورد نظر را بر اساس انتظار محقق اجرا کنند، نمونه گیری بزاقی برای توالی یابی DNA برای تعیین ژنوتیپ ها انجام شد. جهت تعیین ژنوتیپ ژن از روش RFLP استفاده شد. هضم آنزیمی در دمای 65 درجه سانتیگراد و به صورت Overnight شامل یک میکرولیتر آنزیم، 3 میکرولیتر محصول PCR، 2 میکرولیتر بافر مخصوص و 15 میکرولیتر آب دیونیزه استفاده شد. نتایج حاصل از بررسی داده ها با استفاده از آزمون t وابسته آنالیز شد. نتایج نشان داد که میزان VO2max در زنانی که دارای ژنوتیپ AT بودند به طور میانگین قبل و بعد از مداخله ورزشی، معنی دار نبوده است (840/0 p =). میزان VO2max در زنانی که دارای ژنوتیپ AT بودند به طور میانگین قبل و پس از مداخله ورزشی، معنی دار نبوده است (633/0 p =). همچنین، میزان VO2max در زنانی که دارای ژنوتیپ AT بودند، پس از مداخله ورزشی، نسبت به ژنوتیپ TT معنی دار نبود. در این تحقیق نشان داده شد که بهبود معنی دار Vo2max به تفاوت های ژنوتیپی آن ها بستگی نداشت و بین ژنوتیپ های AT و TT پلی مورفیسم rs1870377 ژن VEGFR و تغییرات عملکرد هوازی زنان تمرین نکرده چاق بعد از هشت هفته تمرین هوازی رابطه معنی داری مشاهده نشد.

چکیده انگلیسی:

The present study was conducted with the aim of investigating the effect of eight weeks of aerobic training on the AT and TT genotypes of the VEGFR gene polymorphism rs1870377 and the changes in aerobic performance of untrained women. For this purpose, 29 inactive women aged 30 to 45 were randomly selected from volunteer women in Shahriar, Tehran Province. The Subjects did 8 weeks of aerobic training in 5 sessions per week and each session lasted 30 minutes with an intensity of 55 to 75% of reserve heart rate. In the first two weeks, they trained with 55-65% of the maximum heart rate, and in the second two weeks with 60-65% of the maximum heart rate, and in the last 4 weeks with 65-75% of the maximum heart rate. 10 minutes for warming up and 10 minutes for cooling down were considered in each training session. Bruce's seven-step test was used to determine the VO2max before and after the exercises. The test started with a slope equal to 10% and a speed of 2.7 km/min on the treadmill, and each stage was completed in three minutes, so that in the seventh stage, the slope was 22% and the speed was 9.6. Then, among the subjects who were able to perform the desired test based on the researcher's expectation, saliva sampling was done for DNA sequencing to determine the genotypes. The RFLP method was used to determine the gene genotype. Enzymatic digestion at 65 degrees Celsius overnight, including one microliter of enzyme, 3 microliters of PCR product, 2 microliters of special buffer and 15 microliters of deionized water was used. The results of data analysis were analyzed using the dependent t test. The results showed that VO2max in women with AT genotype was not significant before and after exercise intervention (p = 0.840). The amount of VO2max in women with AT genotype was not significant on average before and after exercise intervention (p = 0.633). Also, the amount of VO2max in women with AT genotype, after exercise intervention, was not significant compared to TT genotype. In this research, it was shown that the significant improvement of Vo2max did not depend on their genotypic differences, and between AT and TT genotypes, the rs1870377 polymorphism of the VEGFR gene and the changes in aerobic performance of obese untrained women after eight weeks of aerobic training no significant relationship was observed.

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