The purpose of this study was to determine the relationship between personality type and two-factor theory with work motivation (a case study) among the staff of Islamic Azad University of Khuzestan province through using a descriptive-analytical method. With a statistical population of 2428 non-faculty members from among 15 university units, 332 persons were estimated and chosen based on the Cochran relation by stratified random sampling method. The applied tools included three questionnaires. The research questions consisted of 9 questions, of which questions 1 to 5 were in fact descriptive and explanatory, and questions 6 to 9 were the main research questions; The first question was related to measuring the components of motivation at work, the second question was related to measuring the component of introverted personality, the third question was related to measuring the component of extroverted personality, and the fourth question was related to measuring the factors of motivation, and the fifth question was related to retaining factors that all five questions were accepted with a high value based on the results of factor load and t and descriptive statistics. The sixth and seventh questions were about the relationship between introverted and extroverted personalities with motivation at work. The results of Pearson correlation coefficient indicated that there is a direct and significant relationship between them. The eighth and ninth questions were about the relationship between motivating and retaining factors and motivation at work. The results of Pearson correlation coefficient presented that there is a direct and significant relationship between them. In the last part of the research, some suggestions were presented to be used in the field and by other people. پرونده مقاله