در سال‌های اخیر مایع‌های یونی به‌دلیل ویژگی‌های منحصربه فردشان توجه زیادی را به خود جلب کرده‌اند. این ویژگی‌ها شامل پایداری گرمایی بالا، فشار بخار ناچیز، حلالیت در آب و حلال‌های آلی، گستره وسیع نقطه ذوب و قابلیت بازیافت است. مایع‌های یونی عامل‌دار، افزون‌بر نقش حلال می‌توانند دارای ویژگی زیستی مؤثری باشند. در این پژوهش، یک روش ساده و کارامد برای تهیه دو مایع یونی کایرال 1- (4- (((1-کربوکسی‌اتیل)کربامویل)بنزیل)پیریدینیم کلرید CECBPy]Cl] و1- (4-(((1کربوکسی‌اتیل)کرباموتیویل)بنزیل) پیریدینیم کلرید CECTCBPY]Cl] از اسید آمینه طبیعی آل(+) آلانین ارائه‌شده است. ساختار این ترکیب‌ها با استفاده از طیف‌سنجی IR،ا1HNMR و 13CNMRمورد تأیید قرار گرفتند. پس از سنجش چرخش نوری و بررسی ویژگی ضد باکتری ترکیب‌های سنتز شده به‌روش تعیین کمترین غلظت مهارکننده رشد در محیط کشت مایع انجام شد. در ادامه، از روش اندازه‌گیری مقدار بازداری رادیکال‌های آزاد 2،2-دی فنیل-1-پیکریل هیدرازیل (DPPH ) برای سنجش اثر ضداکسیداتی نمونه‌ها استفاده شد. نتایج نشان داد که در بین مواد سنتز شده ، 1- (4- (((1-کربوکسی‌اتیل)کرباموتیویل)بنزیل)پیریدینیم کلرید CECTCBPY]Cl] اثر ضداکسیداتی بیشتری دارد. پرونده مقاله

This study was aimed to investigating the antioxidant and cellular toxicity of Symphytum kurdicum. The methanolic extracts of the aerial parts of the plant were prepared through soaking and non-polar to polar cutting of the extract by the liquid-liquid cutting method. The antioxidant effect of the samples was specified by the methods of determining the free radical scanenging 2, 2-diphenyl-1-picrylhydrazyl(DPPH), ferric reducing ability of plasma (FRAP) and the total phenolic content by folin ciocalteu method. Cellular toxicity of the samples on peripheral blood mononuclear cells (PBMC) was performed by 3-(4,5-dimethylthiazol-2-yl) and 2, 5-diphenyltetrazolium bromide (MTT). The results indicated that ethyl acetate and aqueous fractions with IC50 equal to 33.67 and 29.43 μg/ml, respectively, showed the highest ability in DPPH free radicals Scavenging. Moreover, in the study of ferric iron regeneration, the ethyl acetate fraction with a capability of 280.985± 14.007 mM/mg dry weight of sample showed the best regenerative effect against trolox control. The aqueous and ethyl acetate fractions had the highest total phenolic content with 150.765 ± 0.035 and 130.570 ± 0.056 (Gallic acid milligrams/ gram dry weight of sample), respectively. The results of MTT test revealed that all fractions at a concentration much higher than the effective antioxidant concentrations lacked cellular toxicity, too. Given the role of oxidative stress as a predisposing factor in diseases like diabetes, cancer, and cardiovascular disease, aqueous and ethyl acetate fractions are likely to be introduced as pharmacological supplements. پرونده مقاله

Ninhydrin reacts with poly-phenols in different ratios to produce tetracyclic adducts. Here, pyrogallol was used as a polyphenol compound. In the company of acidic ionic liquid (AIL), there was a selective reaction between the ortho-site of polyphenol and the ninhydrin’s carbonyl group. Mono-adduct (1:1) 3 and bis-adduct (2:1) 4 were prepared as a solvent and a catalyst for the reaction of ninhydrin with pyrogallol in 1-(carboxymethyl)-3-methyl-1H-imidazolium chloride (mcmimCl). Purity of the products was approved by 1H NMR, 13C NMR, IR and Mass Spectroscopy. Moreover, the antioxidant activity of novel derivatives investigated in this paper by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical assay and butylated hydroxytoluene (BHT) was considered to be standard. The results indicated that mono-adduct showed the strongest antioxidant activity (IC50 = 5.289µg/ml). پرونده مقاله

This study focused on the biological properties of Cruciata taurica, a plant found in the Irano-Turanian region, which has not been extensively studied in terms of its phytochemical and biological activities. The methanolic extract of the plant was obtained and its cytotoxic, antibacterial, antidiabetic, and antioxidant properties were investigated. The antibacterial activity of the fractions was evaluated using broth microdilution method, while the antioxidant properties were assessed through the DPPH assay and the cytotoxic properties were determined using the RRA assay. This study revealed that the residual aqueous fraction exhibited the highest extraction yield and the most potent antioxidant activity. The EtOAc fraction showed strong antibacterial activity against certain bacteria, while the n-hexane fraction exhibited greater antidiabetic effects. Additionally, the EtOAc fraction was found to be the most cytotoxic. Overall, the study suggested that C. taurica has potential therapeutic applications due to its various biological properties. پرونده مقاله