Two experiments were conducted to evaluate the relationships between nutrients degradability, kinetics of hydration, functional specific gravity (FSG) of the three types of beet pulp (BP) including fine (FBP), normal (NBP) and pelleted (PBP) BP. In experiment 1, about 3 g of samples was weighed in sealed nylon bags (6 cm×7.5 cm, 40±5 µm pore size), incubated in rumen of two cannulated Holstein steers at 0, 3, 6, 12, 18, 24, 36 and 48 h. The dry matter (DM) degradation was different among the treatments for soluble, slowly and potentially degradable fractions, rate of degradation, and effective degradability. In experiment 2, after ruminal incubation of two bags at 0, 0.5, 1, 1.5, 3, 6, 12, 18, 24, 36 and 48 h, the bags were removed without and with washing, the kinetic of hydration, functional specific gravity (FSG) measured with pycnometer. Hydration rate and water holding capacity (WHC) were different. Grinding and pelleting decreased hydration rate and WHC of BP, but increased initial and final FSG over incubation time. Soluble, slowly degradable, and indigestible fraction of DM explained 82.4, 94.8, 2.7, 54.2, 87.3 and 79.7%; 34.1, 50.0, 2.2, 31.4, 62.2 and 63.4%; and 89.1, 12.2, 68.0, 84.7 and 92.9% of the total variation of the fractional rate of degradation, effective degradability, hydration rate, WHC, initial and final FSG, respectively. In addition, the correlations between digestion and hydration parameters were high. As BP has lower than critical size, can easily pass from the reticulorumen orifice, therefore, its FSG is more important to control ruminal retention time and degradation. پرونده مقاله

Two experiments were carried out to evaluate the effects of indigestible neutral detergent fiber (iNDF) of ration for estimation of rumen degradability of fiber in sheep. In experiment 1, to determine feed iNDF, four ruminal fistulated Zel ewes were used. Three grams of alfalfa hay, corn silage, barley grain, wheat bran, ration 1 (including alfalfa hay and concentrate), and ration 2 (including corn silage and concentrate) in four replications were put in nylon bags and incubated in the rumen for 288 h. In experiment 2, the ruminal degradation of dry matter (DM), NDF, iNDF and potential digestible NDF (pdNDF) of alfalfa hay, corn silage, in both ration 1 and 2, were determined with in situ method using four ruminal fistulated Zel ewes. Rumen disappearance of DM at 0 and 2 hour incubation time and NDF at 96 hour incubation time in ration 2 were higher. The soluble, slowly degraded fractions, and rate of degradation in rations 1 and 2 were 28.93 and 25.93%; 50.40 and 46.26%; 2.36 and 3.19 %/h for DM; 11.81 and 11.74%; 61.37 and 46.32%, and 1.74 and 2.33 %/h for NDF, respectively. The iNDF content in alfalfa hay, corn silage, barley grain, wheat bran, ration 1 and 2 were 30.74 ± 3.02, 16.84 ± 2.14, 4.77 ± 1.48, 11.75 ± 2.29, 21.32 ± 0.77, and 11.42 ± 0.91% of DM, respectively. The results showed that despite the similarities in NDF content in both rations, difference in the iNDF content could affect on degradability in rumen. پرونده مقاله

In order to determine of physical characteristics of rice straw as an effective source of fiber in ruminants, alfalfa hay, four varieties of rice straw (Taroum Neda, Taroum Neamat, Taroum Sangi, and Asgari), and four rations that contained four varieties of rice straws were investigated. The chemical (dry matter (DM), organic matter (OM), neutral detergent fiber (NDF), nonfiber carbohydrates (NFC) and crude protein (CP)), and physical characteristics (bulk density, water holding capacity (WHC), and soluble and insoluble DM and ash of samples, kinetics of hydration and change in functional specific gravity (FSG) and feed particle size, physically effective factor (pef)) of forages and total mixed ration (TMR) were determined. Except on ether extract and ash content, the DM, OM, NDF, NFC, and CP content of four rice straw and rations were similar but there was different among alfalfa and rice straws. The rice straws had a bulk density lesser than alfalfa. However, TMR had a similar bulk density, WHC, hydration rate, insoluble DM and ash and greater than alfalfa hay. Alfalfa had lesser WHC than rice straws and there were not different in straws. The soluble DM and FSG of rice straws were similar and lesser than those of alfalfa hay. The TMR had similar physical characteristics. Alfalfa hay had greater FSG than rice straw at all incubation times. Four rice straws and four rations were similar in indegradable NDF (iNDF)and total tract NDF digestibility (TTNDFD). Results showed that regardless the system, rice straws were similar in physically effectiveness and physically more effective than alfalfa because of having greater NDF and iNDF content, geometric mean and pef than alfalfa. پرونده مقاله

Beet pulp as component of cattle feed can be processed in different way. Tis study was performed to characterize the physical properties of so called fine beet pulp (FBP); normal beet pulp (NBP) and pelleted beet pulp (PBP). The following parameters were determined: bulk density, kinetics of hydration, functional specific gravity (FSG), water holding capacity (WHC), soluble dry matter and intrinsic osmotic pressure (IOP) were investigated. Furthermore particle size distribution and its geometric mean were determined according to ASAE S424.1. All three types of beet pulp had similar composition, but different acid detergent insoluble nitrogen, bulk density, WHC, hydration rate, soluble matter and IOP. PBP had the highest (0.967 g/mL) and NBP had the lowest (0.623 g/mL) bulk density. WHC was 4.318, 5.261, 4.881 (g/DM) and hydration rate 0.0527, 0.0663, 0.0657 (g/DM/Min) for FBP, NBP and PBP, respectively. Grinding and pelleting significantly decreased WHC. Initial FSG of FBP was higher than of NBP and PBP (1.416 vs. 1.371 and 1.384, respectively). FSG changed with incubation time as particle size decreased. Final FSG of all three beet pulp types were similar. FBP had the highest soluble DM (28.61 vs. 17.98 and 23.66% of initial DM in NBP and PBP, respectively). In addition, FBP had the highest soluble ash (45.18 vs. 37.79 and 39.36% of initial ash in NBP and PBP, respectively). FBP had the highest IOP and there are not significant differences between NBP and PBP. The studied physical properties were highly correlated with the chemical composition of the pulp. So was bulk density negatively correlated with the neutral detergent fiber (NDF), crude protein (CP) and non-fiber carbohydrates (NFC), hydration rate and WHC and was positively correlated with the DM, EE, FSG, soluble DM and ash, and IOP. In addition, WHC was positively correlated with dry matter (DM), NDF, NFC, CP and EE, but also negatively correlated with bulk density, FSG, soluble DM, soluble ash and IOP. FSG was highly negatively correlated with DM, NDF, CP, NFC, EE, hydration rate and WHC and positive correlated with bulk density, soluble DM, soluble ash and IOP. The physical properties of beet pulp aid in establishing the nutritive value of feedstuffs for ruminant. They physical properties of feedstuff take into account the modifying role of the reticulo-ruminal function on the speed of the character of the quantitative and qualitative biochemical degradation process. پرونده مقاله

This experiment was carried out to evaluate the effect of specific gravity (SG) and particle size (PS) of Chromium-mordanated alfalfa neutral detergent fiber (NDF) markers on estimation of particulate rumen turnover. Thirty-two multiparous, mid lactation Holstein dairy cows (body weight=654±24 kg) were allotted to a completely randomized design experiment with two replications in a 4×4 factorial method. The experiment was accomplished over 21 d (adaptation, 14 d; sample collection, 7 d). Cows were ad libitum fed twice daily at 09:00 and 21:00 with similar total mixed rations. To prepare the marker, alfalfa in the individual bales was chopped for theoretical cut length 19, 10 and 5 mm. The fine alfalfa was prepared by grinding the 5 mm cut alfalfa through a 2 mm screen. The marker preparation followed a modified procedure of Uden et al. (1980) by different PS (8.45, 4.38, 3.00 and 1.10 mm) and K2Cr2O7 concentration in mordanting solution (50, 300, 500 and 900 g/kg K2Cr2O7 instead of 300 g/kg). Fecal grab samples were taken at 0, 6, 12, 16, 20, 24, 30, 36, 48, 60, 72, 96, 120 and 144 h after dosing. Reduction of PS and enhancement of K2Cr2O7 concentration increased SG of marker, ruminal passage rate, but decreased ruminal mean retention time, time delay and total mean retention time. The coefficient of correlation between SG and PS of marker, ruminal passage rate, time delay and ruminal mean retention time were -0.62, -0.70 and 0.65; 0.45, 0.75 and 0.12, respectively. The SG of themarker was the most influential factor affecting ruminal passage rate and mean retention time. پرونده مقاله

Six ruminally fistulated Hereford steers (body weight=414±13 kg) were used in a switch back design to determine whether two particle sizes of alfalfa hay (18.75 and 4.65 mm theoretical cut length) influenced salivary secretion during eating. The experiment carried out in two 26-d periods, with 11-d of adaptation to ration, followed by 5 d for determining the level of voluntary feed intake, 7-d for adaptation to feeding to 90% of voluntary feed intake, and 3-d for measurements. Saliva secretion was measured during the morning meal by rumen evacuation technique at 35 minute after feeding through the rumen fistula of each steer. Coarse and fine alfalfa had the same chemical composition. The geometric mean of coarse and fine alfalfa particles during the eating time decreased by 40.62 and 45.53%, respectively. Reduction of particle size increased the functional specific gravity of alfalfa hay and particulate matter. In addition, the gas associated with particles had similar trend to the functional specific gravity. Production of saliva in milliliters (P=0.001), per kg of dry matter intake (P<0.0001) and per each kilogram of neutral detergent fiber (NDF) intake (P<0.0001) were affected by alfalfa particle size. Saliva production was higher in coarse alfalfa treatment. Reduction of alfalfa particle size reduces the ability of alfalfa forage as a physically effective fiber source in feeding of ruminant by decreasing the ability to saliva secretion. پرونده مقاله

The objective of this study was to investigate the effects of cut and irrigation water quality on chemical composition and in situ dry matter (DM), crude protein (CP) and neutral detergent fiber (NDF) degradability of alfalfa. Three fistulated Zel sheep (approximately 2 years old) were used in a complete randomized design to evaluate the in situ rumen degradability. Ruminal incubation times consisted of 0, 3, 6, 9, 12, 24, 48, and 72 h. The results showed that increasing the cut of maturity and irrigation with saline water significantly cause to increase the components of cell wall (P=0.0011) and decrease the protein concentrations in alfalfa (P=0.0001). Except the quickly rumen degradability of DM that in first cut of alfalfa was higher, other parameters of ruminal degradability of DM were not different. Ruminal NDF degradability in second cut of alfalfa, that irrigated with saline water, was higher (P=0.0143). Degradation rate of NDF in first cut of alfalfa, and effective rumen degradation of NDF at different passage rate in alfalfa that irrigated with saline water were higher. Slowly ruminal CP degradability (P=0.001) and effective degradation at different passage rate of alfalfa, that irrigated with saline water, were higher. The cut did not effect on CP degradability of alfalfa. Our experiment indicates that increasing water salinity have not a negative effect on alfalfa forage quality. پرونده مقاله

A study was conducted to evaluate the effects of energy and protein levels of maternal diets on growth, some blood parameters, health, and performance of baby goat kids at first month of life. On 95 d after successful mating, 28 synchronized singleton pregnant Sistani goats were randomly allocated to four treatments including 1) low protein and low energy (LPLE), 2) high protein and low energy (HPLE), 3) low protein and high energy (LPHE), and 4) high protein and high energy (HPHE) diets. In all treatments, body weight (BW) reduced at 1d and 28 d after party. The birth BW of kids was similar between the four groups but LPLE, LPHE and HPHE had numerically lower birth weight. The kids from nanny goats fed on high protein diets had greater BW and average daily gain (ADG) than those of others on days 7, 14, 21, 28. Plasma triglyceride and total protein were significantly high in HPHE and glucose concentrations were increased in HPLE. Milk production increased in response to prepartum crude protein (CP). The results show that maternal nutrition had a positive effect on weight and biometric traits of kids. پرونده مقاله

Most efficient animals in heat-stressed condition, intelligently regulate their metabolism for maximizing their productivity and fats play an important role in reducing heat stress in these animals but the underlying mechanisms remain elusive. The effects of different fat sources on some blood metabolites and hormones and enzyme activities of heat-stressed lambs with different residual feed intake (RFI) were studied. In the preliminary trial, 48 male lambs of four different breeds (Zel, Dalagh, and their hybrids with Romanov; BW=16.87±0.55 kg) in a block randomized complete design used to assay RFI for 67 days. After that, 32 lambs of the same breeds as a block (BW=30.74±1.21 kg) with two different RFIs in heat stressed condition were used in a 2 × 4 factorial trail over 84 days. The treatments included four rations: 1) basal ration (control), 2, 3, and 4) rations supplemented with calcium soap fatty acids (FA), beef tallow, and canola oil, respectively. Dry matter intake (DMI) was high in control and high RFI groups (low efficient) (p < 0.05). Lipid sources had significant effect on serum glucose, cholesterol, triiodothyronine (T3), and Insulin, pulse and respiration rate (p < 0.05). No differences found between treatments for triglyceride, thyroxine(T4), low-density lipoprotein (LDL), and Very-low-density lipoprotein (VLDL). Glucose concentration had strong correlation with the RFI (p < 0.01). The Low RFI lambs (LRFI) had high T3 and low LDH levels. These lambs had high respiration and pulse rate (p < 0.05). Lambs fed with fat supplemented rations had higher concentration of serum aspartate aminotransferase (AST), alkaline phosphatase (ALP), and lactic dehydrogenase (LDH) enzymes than control (p < 0.01). Lipid supplementation in heat stressed lambs markedly alters glucose, cholesterol, T3 and respiration and pulse rate that independently of reduced dry matter intake (DMI) through coordinated changes in fuel supply and utilization by multiple tissues. Even more challenging the most efficient lambs (LRFI) had high physiologic rate and high activity for removing heat from tissues. پرونده مقاله