بررسی فعالیت آنتی‌اکسیدانی و ترکیبات فیتوشیمیایی میوه گیاه دارویی Rubus ulmifolius sub sp. sanctus جمع‌آوری شده از منطقه خان‌دره‌سی ارومیه

محورهای موضوعی : گیاهان دارویی

شیرین رحمن زاده ایشکه ¹ (گروه باغبانی- دانشکده کشاورزی- دانشگاه ارومیه- ایران)
محمدرضا اصغری ² (استاد گروه علوم باغبانی،دانشکده کشاورزی، دانشگاه ارومیه، ایران)
حبیب شیرزاد ³ (استاد یار گروه علوم باغبانی- دانشکده کشاورزی- دانشگاه ارومیه- ایران)
ابوالفضل علیرضالو ⁴ (استادیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه)

تاریخ دریافت : 1396/09/29 تاریخ پذیرش : 1396/12/14 تاریخ انتشار : 1399/12/01

کلید واژه: آنتی‌اکسیدان, فنل کل, فلاونوئید, آنتوسیانین کل, تمشک,

چکیده مقاله :

تمشک (Rubus ulmifolius sub sp. sanctus)، درختچه‌ای است چند ساله، از تیره گلسرخیان که دارای مصارف دارویی و خوراکی بوده و به صورت خودرو در منطقه خان دره‌سی ارومیه رویش و گسترش بسیاری دارد. میوه تمشک به دلیل برخورداری از فعالیت آنتی‌اکسیدانی بالا، انواع فلاونوئیدها و پروآنتوسیانین‌ها اهمیت زیادی در صنایع غذایی و دارویی دارد. در این تحقیق پس از برداشت میوه‌ها از منطقه خان دره‌سی ارومیه در اواخر تابستان سال 1395، میزان اسیدیته به وسیله‌یpH ‌متر، اسیدهای آلی به روش تیتراسیون با سود 1/0 نرمال، مواد جامد محلول با استفاده از دستگاه رفراکتومتر، فنل کل به روش فولین سیوکالتیو، فلاونوئید کل با استفاده از کلرید آلومنیوم، فعالیت آنتی‌اکسیدانی به دو روش DPPHو FRAP، آنتوسیانین به روش اختلاف pHها، و همچنین آنزیم‌های L-فنیل آلانین آمونیالیاز (PAL) و گایاکول‌پراکسیداز (G-POD) اندازه‌گیری شد. نتایج نشان دادند که میزان pH، اسیدهای آلی و مواد جامد محلول به ترتیب برابر با 23/3، 14/1 و 6/12 بودند، همچنین محتوای فنل و فلاونوئید کل به ترتیب 44/26 میلی‌گرم اسیدگالیک بر 1 میلی‌لیتر عصاره و 940/1 میلی‌گرم کوئرسیتین بر 1 میلی‌لیتر بودند. همچنین میزان فعالیت آنتی‌اکسیدانی اندازه‌گیری شده و میزان آنتوسیانین کل این میوه دارویی به ترتیب برابر با 29/55 درصد و 789/63 میلی‌گرم سیانیدین 3-گلیکوزید در 1 میلی‌لیتر عصاره بودند. نتایج این تحقیق نشان داد که میوه تمشک دارای محتوای بالایی از ترکیبات پلی‌فنلی بوده و فعالیت آنتی‌اکسیدانی قابل توجهی دارد، بنابراین می‌تواند در صنایع غذایی و دارویی کاربرد فراوانی داشته باشند.

چکیده انگلیسی:

The raspberry (Rubus ulmifolius subsp. Sanctus) is the perennial shrub in the genus Rubus of the Rosacea family. It is a medicinal and edible fruit throughout much of Khandaracy region of Urmia. Raspberry fruits are most important in food and medicinal sciences due to their excellent antioxidant activity and various flavonoids and proanthocyanin content. In this research, after harvesting fruits from the Khandaracy region of Urmia in the late summer, pH level by pH meter, organic acids by titration with 0.1 molar NaOH, Soluble solids using a refractometer, total phenolic content (by using Folin–Ciocalteu assays), total flavonoid content (aluminum chloride method), antioxidant capacity by two methods (DPPH and FRAP), total anthociyanin content (pH differential method), L-phenylalanine ammonia-lyase (PAL) and (guaiacol peroxidase) G-POD enzymes were determined. The results showed that level pH, organic acids and soluble solids were respectively 3.13, 1.14, 12.6, also values of total phenol content, total flavonoid, antioxidant activity and total anthocyanin content were 26.44 mg GAE.ml-1 Extract, 1.99 mg Qu. ml-1 extract, 55.29 % and 63.78 cyanidin-3-glucoside ml-1 Extract respectively. The results of this study showed that raspberry fruit has high content of polyphenol compounds and has significant antioxidant activity, so it can be used in food and pharmaceutical industries.

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