استخراج کیفی و کمی استویوزید از گیاه دارویی Stevia rebadiana Bertoni. با استفاده از روش فاز جامد قالب مولکولی

محورهای موضوعی : شیمی و آنالیز ترکیبات طبیعی

سمیه آقابیک ¹ (گروه شیمی، دانشکده علوم، واحد گرگان، دانشگاه آزاد داسلامی، گرگان، ایران)
مازیار احمدی گلسفیدی ² (گروه شیمی، دانشکده علوم، واحد گرگان، دانشگاه آزاد داسلامی، گرگان، ایران)
محمد هادی سلیمانی ³ (داروسازی گیاه اسانس، گرگان، ایران)

تاریخ دریافت : 1395/11/08 تاریخ پذیرش : 1396/02/19 تاریخ انتشار : 1396/06/01

کلید واژه: کروماتوگرافی مایع با کارایی بالا, استویا, استخراج فاز جامد, استویوزید, پلیمر قالب مولکولی, پلاروگرافی,

چکیده مقاله :

گیاه استویا با نام علمی Stevia rbaudiana Bertoni. حاوی شیرین کننده استویول ، گلیکوزیدی بدون کالری است که با اثر بر تنظیم میزان قند خون به خصوص در بیماران دیابتی به‌عنوان یک داروی موثر عمل می نماید. هدف از انجام این تحقیق دستیابی به یک روش نوین جهت جداسازی کیفی و کمی استویوزید به‌عنوان شیرین کننده بدون کالری از گیاه دارویی استویا می‌باشد.در این تحقیق نمونه برگهای خشک شده گیاه استویا در شهریورماه 1394 از شرکت تولید کننده گلساران شمال تهیه شد. به‌منظور استخراج استویوزید علاوه بر روش HPLC و پلاروگرافی، یک فاز جامد قالب مولکولی مناسب تهیه و سپس برخی از عوامل موثر بر استخراج توسط این روش نوین، بهینه سازی گردید. برای تهیه پلیمر استخراج‌گر، از یک مونومر عاملی اکریلاتی و یک اتصال دهنده عرضی استفاده شد. نسبت کمی اجزای تشکیل‌دهنده پلیمر و همچنین تاثیر درجه ریز شدگی فاز استخراج‌گر بر راندمان استخراج نیز مورد ارزیابی قرار گرفت. نتایج نشان داد که بیشترین درصد بازدهی استخراج استویوزید در نسبت 1 به 5 از مقدار مونومر عاملی به مقدار اتصال‌دهنده عرضی و بهترین درجه ریز شدگی این استخراج گر 1000 میکرون به‌دست آمد و میزان استویوزید نیز 11.16 درصد وزنی گزارش گردید.

چکیده انگلیسی:

Stevia rebaudiana Bertoni. with non-calorie steviol glycoside  is an effective drug to decrease of blood glucose in treat of  diabetics. The goal of this study is obtaining of  a novel method in order to a quantitative and qualitative extraction of stevioside as a non-calorie sweetener from Stevia. In this study the dry leaves of Stevia was prepared from Golsaran Shomal company and then in order to extraction of stevioside a solid phase molecularly imprinted extractor was prepared and some parameters affective on extraction of stevioside were optimized. An acrylate functional monomer and a cross linker were used to preparation of stevioside extractor polymer. Functional monomer to cross linker ratio and size and fining degree of the extractor were studied and evaluated. In this study analysis of the stevioside beside of HPLC was accrued by polarography method .The best ratio of functional monomer to cross linker was obtained in 0.2, also the best polymer particle size was obtained in 1000 micron for extraction of stevioside by the extractor and the amount of stevioside was determined 11.6% w/w in this plant.

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