ارزیابی شاخصهای بیوشمیایی گیاه دارویی خرفه oleracea Portulaca تحت تنش کروم و شوری
محورهای موضوعی : ژنتیکزهرا طالب زاده 1 , راحله رهباریان 2 , محبت نداف 3 , حمید سبحانیان 4
1 - گروه زیست شناسی دانشگاه پیام نور تهران ایران
2 - استادیار،گروه زیست شناسی، دانشگاه پیام نور،تهران ایران
3 - گروه زیست شناسی دانشگاه پیام نور تهران ایران
4 - گروه زیست شناسی، دانشگاه پیام نور، تهران ایران
کلید واژه: پرولین, شوری, آنتی اکسیدان, خرفه, دی کرومات پتاسیم, مالون دآلدئید,
چکیده مقاله :
هدف از پژوهش حاضر، بررسی برهمکنش تنش شوری و کروم بر برخی شاخصهای بیوشیمیایی گیاه خرفه بود. این پژوهش درقالب طرح کاملا تصادفی گلخانهای و با 3 تکرارانجام شد. 4 سطح شوری (0، 4، 8 و 12 دسیزیمنس بر متر) بااستفاده از کلریدسدیم هر 4 روز ازطریق آبیاری و سطوح تیمار کروم (0، 7، 14، 21 و 28 میلیگرم بر کیلوگرم وزن خشک خاک) فراهم شد و قبل از کشت بذر در خاک، سطوح مختلف تیمار کروم با افزایش دی کرومات پتاسیم به خاک، تهیه شد. مقادیر کربوهیدراتهای محلول و نامحلول در الکل، پروتئینها، پرولین، مالون دآلدئید و پراکسیدهیدروژن پس از 60 روز از کشت گیاهان اندازهگیری و سنجش شدند. نتایج نشان داد با افزایش سطوح مختلف کروم و شوری مقادیر پرولین، پراکسید، مالون دآلدئید، کربوهیدرات محلول روند افزایشی داشت، اما کربوهیدراتهای نامحلول و پروتئینها روند کاهشی داشت. کمترین میزان کربوهیدراتهای نامحلول و پروتئینها و بیشترین میزان پرولین، پراکسید، مالون دآلدئید، کربوهیدرات محلول در الکل در تنش ترکیبی سطوح شوری 12 دسیزیمنسبرمتر و کروم 28 میلیگرم بر کیلوگرم مشاهده گردید. تنش ترکیبی شوری و کروم باعث کاهش بیشتری در کربوهیدراتهای نامحلول و پروتئینها خرفه گردید.
The aim of this study was to investigate the interaction of salinity and chromium stress on some biochemical characteristics of portulaca oleracea. This study was conducted in a completely randomized greenhouse design with 3 replications. 4 salinity levels (0, 4, 8 and 12 ds/m) were provided using sodium chloride every 4 days through irrigation and chromium treatment levels (0, 7, 14, 21 and 28 mg/kg dry weight of soil) and before from seed cultivation in soil, different levels of chromium treatment were obtained by increasing potassium dichromate to soil. The levels of Soluble and insoluble carbohydrates in alcohol, proteins, proline, malondialdehyde and hydrogen peroxide were measured after 60 days of plant cultivation. The results showed that with increasing levels of chromium and salinity, the amounts of proline, peroxide, malondialdehyde, soluble carbohydrates increased, but insoluble carbohydrates and proteins decreased. The lowest amount of insoluble carbohydrates and proteins and the highest amount of proline, peroxide, malondaldehyde and alcohol-soluble carbohydrates were observed in the combined stress of salinity levels of 12 ds/m and chromium 28 mg/kg. The combined stress of salinity and chromium caused a further reduction in insoluble carbohydrates and portulaca protein.
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