ارزیابی تأثیر مقایسه‌ای غلظت‌های مختلف عصاره هیدروالکلی بابونه بر کیفیت اسپرم منجمد-یخ‌گشایی‌شده در قوچ
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
حسین دقیق کیا
1
,
فاطمه صادقی صادق آباد
2
,
مرضیه ابراهیمی
3
,
فرهاد صمدیان
4
1 - استاد گروه علوم دامی دانشکده کشاورزی دانشگاه تبریز
2 - دانش‌آموخته کارشناسی ارشد گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
3 - استادیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
4 - استادیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه یاسوج، یاسوج، ایران.
تاریخ دریافت : 1395/06/07
تاریخ پذیرش : 1396/02/20
تاریخ انتشار : 1396/02/01
کلید واژه:
بابونه,
اسپرم,
استرس اکسیداتیو,
آنتی‌اکسیدان طبیعی,
انجماد-یخ‌گشایی,
چکیده مقاله :
استرس اکسیداتیو به وجودآمده در طول فرآیند انجماد-یخ‌گشایی اسپرم باعث کاهش تحرک، زنده مانی، عملکردهای غشایی و در نهایت باروری سلول های اسپرم می شود. هدف این مطالعه بررسی تأثیر عصاره گیاه بابونه به‌عنوان آنتی اکسیدان طبیعی بر کیفیت اسپرم منجمد-یخ گشایی شده قوچ بود. در این مطالعه از 5 رأس قوچ دو بار در هفته توسط واژن مصنوعی اسپرم گیری شد و جهت از بین بردن اثرات فردی دام ها، انزال ها به نسبت مساوی باهم مخلوط شدند. سطوح مختلف عصاره گیاه بابونه (صفر، 50، 66/66، 100، 200 میلی‌لیتر در دسی لیتر) به رقیق کننده بر پایه زرده تخم‌مرغ-تریس افزوده شد. نمونه ها بعد از طی مراحل سردسازی و پرشدن در پایوت‌ها، در بخار ازت منجمد شده و تا زمان ارزیابی در ازت مایع نگه داری شدند. نتایج ارزیابی نشان داد که افزودن 66/66 میلی‌لیتر در دسی لیتر عصاره بابونه باعث بهبود معنی دار صفات تحرک کل، تحرک پیش رونده، سرعت در مسیر منحنی، سرعت در مسیر مستقیم، راستی مسیر طی شده ، زنده مانی و یکپارچگی غشای پلاسمایی بعد از فرآیند انجماد-یخ گشایی نسبت به گروه شاهد و سایر گروه‌های تیماری شد (05/0p<). همچنین افزودن 200 میلی‌لیتر در دسی لیتر عصاره به‌طور معنی داری اثر منفی روی صفات تحرکی، زنده مانی و یکپارچگی غشای پلاسمایی داشت (05/0p<). استفاده از سطوح مختلف عصاره گیاه بابونه موجب کاهش معنی دار غلطت مالون‌دی‌آلدئید نسبت به گروه شاهد نشد ولی کاهش تولید مالون دی آلدئید در سطح 66/66 میلی‌لیتر در دسی لیتر نسبت به سطوح 100 و 200 میلی‌لیتر در دسی لیتر معنی دار بود.
چکیده انگلیسی:
Oxidative stress during freeze-thawing process causes reduction in motility, viability, membrane functions and finally sperm fertility. The aim of this study was to determine the effect of Chamomile extract as natural antioxidant on quality of cryopreserved ram sperm. In this study semen was collected from five mature rams twice a week using an artificial vagina and the ejaculates were pooled equally in order to eliminate the individual effects. Different levels of extract of Chamomile (0, 50, 66.66, 100 and 200 ml/dl of diluent solution) were added to diluent based tris-egg yolk. After cooling, filling and sealing of the samples, they were frozen with nitrogen vapor and immersed in liquid nitrogen and were stored until evaluation time. After thawing, results showed that addition of 66.66 ml/dl of extract increased total motility, progressive motility, curvilinear velocity, straight–line velocity, linearity, viability and plasma membrane integrity of sperm compared to the control and other treatment groups (p<0.05). Also addition of 200 ml/dl extract had significant negative effects on motility parameters, viability and plasma membrane integrity of sperm (p<0.05). Inclusion of different doses of Chamomile extract caused no significant reduction on malondialdehyde concentration in comparison to control but this reduction was significant at 66.66 ml/dl in comparison to 100 and 200 ml/dl of extract. Conflict of interest: None declared.
منابع و مأخذ:
Aggag, M. and Yousef, R. (1972). Study of antimicrobial activity of chamomile oil. Planta Medica, 22(06): 140-144.
Arora, A., Byrem, T.M., Nair, M.G. and Strasburg, G.M. (2000). Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Archives of Biochemistry and Biophysics, 373(1): 102-109.
Bailey, J.L., Bilodeau, J.F. and Cormier, N. (2000). Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon. Journal of Andrology, 21: 1-7.
Bansal, A.K. and Bilaspuri, G.S. (2010). Impacts of oxidative stress and antioxidants on semen functions. Veterinary Medicine International, 686137: 1-7.
Baumber, J. and Ball, B.A. (2005). Determination of glutathione peroxidase and superoxide dismutase-like activities in equine spermatozoa, seminal plasma, and reproductive tissues. American Journal of Veterinary Research, 66(8): 1415-1419.
Bilodeau, J.F., Blanchette, S., Cormier, N. and Sirard, M.A. (2002). Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender: protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase. Theriogenology, 57(3): 1105-1122.
Blokhina, O., Virolainen, E. and Fagerstedt, K.V. (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany, 91(2): 179-194.
Daghigh Kia, H., Olfati Karaji, R., Ashrafi, I. and Hosseinkhani, A. (2014). Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation. Spanish Journal of Agricultural Research, 12(1): 98-105.
Daghigh Kia, H., Farhadi, R., Ashrafi, I. and Mehdipour, M. (2016). Anti‐oxidative effects of ethanol extract of Origanum vulgare on kinetics, microscopic and oxidative parameters of cryopreserved Holstein bull spermatozoa. Iranian Journal of Applied Animal Science, 6(4): 901-907.
Gardiner, P. (2007). Complementary, holistic, and integrative medicine: chamomile. Pediatrics in Review, 28(4): e16-18.
Hatami, L. and Estakhr, J. (2013). The Effects of Hydroalcoholic Extract of Matricaria Recutita on the Hormonal Pituitary-Testis Axis and Testis Tissue Changes of Mature Male Rats. Journal of Fasa University of Medical Sciences, 3(1): 56-62. [In Persian]
Malo, C., Gil, L., Cano, R., Martinez, F. and Gale, I. (2011). Antioxidant effect of rosemary (Rosmarinus officinalis) on boar epididymal spermatozoa during cryopreservation. Theriogenology, 75(9): 1735-1741.
Masoumi Ardakani, Y., Abbasnejad, M., Derakhshanfar, A., Esmaeilpour Bezenjani, Kh. and Mostafavi A. (2010). Effect of Matricaria Recutita L. aqueous extract on acetic acid-induced ulcerative colitis in adult male rats. Physiology and Pharmacology, 14(3): 269-280. [In Persian]
Mehdipour, M., Daghigh Kia, H., Najafi, A., Dodaran, H.V. and García-Álvarez, O. (2016). Effect of green tea (Camellia sinensis) extract and pre-freezing equilibration time on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology, 73(3): 297-303.
Najafi, A., Daghigh Kia, H., Mohammadi, H., Najafi, M.H., Zanganeh, Z. and Sharafi, M., et al. (2014). Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender. Cryobiology, 69(1): 68-73.
Najafi, A., Zhandi, M., Towhidi, A., Sharafi, M., Akbari Sharif, A. and Khodaei Motlagh, et al. (2013). Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology, 66(3): 275-282.
Revell, S. and Mrode, R. (1994). An osmotic resistance test for bovine semen. Animal Reproduction Science, 36(1-2): 77-86.
Roca, J., Gil, M.A., Hernandez, M., Parrilla, I., Vazquez, J.M. and Martinez, E.A. (2004). Survival and fertility of boar spermatozoa after freeze-thawing in extender supplemented with butylated hydroxytoluene. Journal of Andrology, 25(3): 397-405.
Sadighara, P., Barin, A., Jahed, Gh. and Farjadmand, F. (2013). Assessment of Antioxidant Capacity and Anti-Inflammatory of Alcoholic Extraction of Chamomile, Morus, Marshmallow, Borage and Rosemary. Knowledge and Health, 8(1):31-34. [In Persian]
Sapanidou, V.G., Margaritis, I., Siahos, N., Arsenopoulos, K., Dragatidou, E. Taitzoglou, I.A., et al. (2014). Antioxidant effect of a polyphenol-rich grape pomace extract on motility, viability and lipid peroxidation of thawed bovine spermatozoa. Journal of Biological Research-Thessaloniki, 21:19.
Tasdemir, U., Buyukleblebici, S., Tuncer, P.B., Coskun, E., Ozgurtas, T. Aydin, A., et al. (2013). Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters. Cryobiology, 66(1): 38-42.
Tubaro, A., Zilli, C., Redaelli, C. and Della Loggia, R. (1984). Evaluation of anti-inflammatory activity of a chamomile extract after topical application. Planta Medica, 50(04): 359-359.
Zanganeh, Z., Zhandi, M., Zare-Shahneh, A., Najafi, A., Nabi, M.M. and Mohammadi-Sangcheshmeh, A. (2013). Does rosemary aqueous extract improve buck semen cryopreservation? Small Ruminant Research, 114(1): 120-125.
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Aggag, M. and Yousef, R. (1972). Study of antimicrobial activity of chamomile oil. Planta Medica, 22(06): 140-144.
Arora, A., Byrem, T.M., Nair, M.G. and Strasburg, G.M. (2000). Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Archives of Biochemistry and Biophysics, 373(1): 102-109.
Bailey, J.L., Bilodeau, J.F. and Cormier, N. (2000). Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon. Journal of Andrology, 21: 1-7.
Bansal, A.K. and Bilaspuri, G.S. (2010). Impacts of oxidative stress and antioxidants on semen functions. Veterinary Medicine International, 686137: 1-7.
Baumber, J. and Ball, B.A. (2005). Determination of glutathione peroxidase and superoxide dismutase-like activities in equine spermatozoa, seminal plasma, and reproductive tissues. American Journal of Veterinary Research, 66(8): 1415-1419.
Bilodeau, J.F., Blanchette, S., Cormier, N. and Sirard, M.A. (2002). Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender: protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase. Theriogenology, 57(3): 1105-1122.
Blokhina, O., Virolainen, E. and Fagerstedt, K.V. (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany, 91(2): 179-194.
Daghigh Kia, H., Olfati Karaji, R., Ashrafi, I. and Hosseinkhani, A. (2014). Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation. Spanish Journal of Agricultural Research, 12(1): 98-105.
Daghigh Kia, H., Farhadi, R., Ashrafi, I. and Mehdipour, M. (2016). Anti‐oxidative effects of ethanol extract of Origanum vulgare on kinetics, microscopic and oxidative parameters of cryopreserved Holstein bull spermatozoa. Iranian Journal of Applied Animal Science, 6(4): 901-907.
Gardiner, P. (2007). Complementary, holistic, and integrative medicine: chamomile. Pediatrics in Review, 28(4): e16-18.
Hatami, L. and Estakhr, J. (2013). The Effects of Hydroalcoholic Extract of Matricaria Recutita on the Hormonal Pituitary-Testis Axis and Testis Tissue Changes of Mature Male Rats. Journal of Fasa University of Medical Sciences, 3(1): 56-62. [In Persian]
Malo, C., Gil, L., Cano, R., Martinez, F. and Gale, I. (2011). Antioxidant effect of rosemary (Rosmarinus officinalis) on boar epididymal spermatozoa during cryopreservation. Theriogenology, 75(9): 1735-1741.
Masoumi Ardakani, Y., Abbasnejad, M., Derakhshanfar, A., Esmaeilpour Bezenjani, Kh. and Mostafavi A. (2010). Effect of Matricaria Recutita L. aqueous extract on acetic acid-induced ulcerative colitis in adult male rats. Physiology and Pharmacology, 14(3): 269-280. [In Persian]
Mehdipour, M., Daghigh Kia, H., Najafi, A., Dodaran, H.V. and García-Álvarez, O. (2016). Effect of green tea (Camellia sinensis) extract and pre-freezing equilibration time on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology, 73(3): 297-303.
Najafi, A., Daghigh Kia, H., Mohammadi, H., Najafi, M.H., Zanganeh, Z. and Sharafi, M., et al. (2014). Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender. Cryobiology, 69(1): 68-73.
Najafi, A., Zhandi, M., Towhidi, A., Sharafi, M., Akbari Sharif, A. and Khodaei Motlagh, et al. (2013). Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology, 66(3): 275-282.
Revell, S. and Mrode, R. (1994). An osmotic resistance test for bovine semen. Animal Reproduction Science, 36(1-2): 77-86.
Roca, J., Gil, M.A., Hernandez, M., Parrilla, I., Vazquez, J.M. and Martinez, E.A. (2004). Survival and fertility of boar spermatozoa after freeze-thawing in extender supplemented with butylated hydroxytoluene. Journal of Andrology, 25(3): 397-405.
Sadighara, P., Barin, A., Jahed, Gh. and Farjadmand, F. (2013). Assessment of Antioxidant Capacity and Anti-Inflammatory of Alcoholic Extraction of Chamomile, Morus, Marshmallow, Borage and Rosemary. Knowledge and Health, 8(1):31-34. [In Persian]
Sapanidou, V.G., Margaritis, I., Siahos, N., Arsenopoulos, K., Dragatidou, E. Taitzoglou, I.A., et al. (2014). Antioxidant effect of a polyphenol-rich grape pomace extract on motility, viability and lipid peroxidation of thawed bovine spermatozoa. Journal of Biological Research-Thessaloniki, 21:19.
Tasdemir, U., Buyukleblebici, S., Tuncer, P.B., Coskun, E., Ozgurtas, T. Aydin, A., et al. (2013). Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters. Cryobiology, 66(1): 38-42.
Tubaro, A., Zilli, C., Redaelli, C. and Della Loggia, R. (1984). Evaluation of anti-inflammatory activity of a chamomile extract after topical application. Planta Medica, 50(04): 359-359.
Zanganeh, Z., Zhandi, M., Zare-Shahneh, A., Najafi, A., Nabi, M.M. and Mohammadi-Sangcheshmeh, A. (2013). Does rosemary aqueous extract improve buck semen cryopreservation? Small Ruminant Research, 114(1): 120-125.
