آسیب‌شناسی بافتی بیماری طاعون نشخوارکنندگان کوچک در بزها و گوسفندان منطقه سیستان
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
یاسمن درخشان
1
(دانش‌آموخته دکترای حرفه‌ای دامپزشکی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران.)
سیده آیدا داوری
2
(استادیار گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران.)
علی سارانی
3
(استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران.)
تاریخ دریافت : 1397/04/01
تاریخ پذیرش : 1398/01/27
تاریخ انتشار : 1398/09/01
کلید واژه:
گوسفند,
سیستان,
بز,
آسیب‌شناسی بافتی,
طاعون نشخوارکنندگان کوچک,
چکیده مقاله :
طاعون نشخوارکنندگان کوچک (peste des petits ruminants; PPR) بیماری ویروسی واگیردار مهم گوسفند و بز می باشد. با توجه به اهمیت PPR در سیستان، مضرات اقتصادی ناشی از تلفات و هزینه های درمانی، مصرف بالای گوشت دام به شیوه ذبح سنتی و عدم نظارت کافی بر سلامت دام‌ها، مطالعه حاضر با هدف آسیب شناسی بافتی بیماری مذکور در جمعیت گوسفند و بز منطقه سیستان صورت گرفت. از 30 رأس گوسفند و بز گله های مشکوک به بیماری، نمونه های بافتی از دهان، ریه، روده‌ها و غدد لنفاوی جمع‌آوری و پس از تهیه مقطع مورد مطالعه میکروسکوپی قرار گرفتند. تعداد 9 نمونه دارای آسیب های بافتی بودند.داده ها با نرم‌افزار SPSS و آزمون کاپا مورد تحلیل آماری قرار گرفت. میزان آلودگی در گوسفند و بز به ترتیب 25/33 و 88/38 درصد بود که ارتباط آماری معنی داری بین گونه دامی و آسیب های بافتی همچون تجمع سلول‌های آماسی چندهسته‌ای و تشکیل سلول‌های سینسیتیا مشاهده شد (05/0>p ). همچنینمیزان عفونت در سنین زیر دو سال و بالای دو سال به ترتیب 18/35و 8/22 درصدبود و ارتباط آماری معنی داری از نظر سن دام و شدت تجمع سلول های آماسی تک هسته‌ای هم وجود داشت (05/0>p ). میزان آلودگی در جنس نر و ماده هم به ترتیب 98/49و 5/12 درصدبود و ارتباط آماریمعنی داری از نظر جنسیت دام و میزان بروز آسیب هایی چون تجمع سلول های آماسی چندهسته‌ای، تشکیل سلول های سینسیتیا وگنجیدگی‌های درون سیتوپلاسمی نیز مشاهده شد (05/0>p ). این مطالعه نشان داد که PPRدر منطقه سیستان در بزهای زیر دو سال با احتمال وقوع بیشتر و ضایعات پاتولوژیک شدیدتری همراه است.
چکیده انگلیسی:
Peste-des-petits ruminants, is an important viral contagious disease of sheep and goat. Considering the importance of PPR in Sistan region, its economic losses as a consequence of mortality and treatment expenses, the high consumption of animal meat in the traditional way of slaughter in this area and lack of adequate supervision on animal health, the present study was conducted with the aim of histopathological investigation of this disease in sheep and goats of Sistan region. Tissue specimens from mouth, lungs, intestines and lymph nodes of 30 cases from suspected flocks were collected and after tissue processing were microscopically examined. In microscopic investigation, lesions were observed in 9 specimens. The data were analyzed using SPSS software and Kappa test. The incidence of infection in sheep and goats was 33.25% and 38/88% respectively and significant association was observed between species and lesions such as multinucleated inflammatory cells accumulation and syncytial cells formation (p < /em><0.05). Also, the infection rates in ruminants less than and more than two years old were 35.18% and 22.8%, respectively and significant statistical relationship was found between the age and the severity of mononuclear inflammatory cells accumulation (p < /em><0.05). The contamination rates in males and females were 49.98% and 12.5%, respectively and significant statistical association was found between the sex and the incidence of lesions like multinucleated inflammatory cells accumulation, syncytial cells and intracytoplasmic inclusion bodies (p < /em><0.05). The results of this study demonstrated that there is a higher incidence of PPR occurrence in Sistan region in goats less than two years old with much severe pathologic lesions.
منابع و مأخذ:
Abdollahpour, G., Raoofi, A., Najafi, J., Sasani, F. and Sakhaie, E. (2006). Clinical and paraclinical findings of a recent outbreaks of Peste des petits ruminants in Iran. Zoonoses and Public Health, 53(s1): 14-16.
Abubakar, M., Ali, Q. and Khan, H.A. (2008). Prevalence and mortality rate of peste des petitis ruminant (PPR): possible association with abortion in goat. Tropical Animal Health and Production, 40(5): 317-321.
Al-Dubaib, M.A. (2009). Peste des petitis ruminants morbillivirus infection in lambs and young goats at Qassim region, Saudi Arabia. Tropical Animal Health and Production, 41(2): 217-220.
Al-Naeem, A., Abu, E.E. and Al-Afaleq, A.I. (2000). Epizootiological aspects of peste des petits ruminants and rinderpest in sheep and goats in Saudi Arabia. Revue Scientifique Et Technique, 19(3): 855-858.
Aruni, A.W., Lalitha, P.S., Mohan, A.C., Chitravelu, P. and Anbumani, S.P. (1998). Histopathological study of a natural outbreak of peste des petits ruminants in goats of Tamilnadu. Small Ruminant Research, 28(3): 233-240.
Atta-ur-Rahman, M.A., Rahman, S.U., Akhtar, M. and Ullah, S. (2004). Peste des petits ruminants antigen in mesenteric lymph nodes of goats slaughtered at DI Khan. Pakistan Veterinary Journal, 24(3): 159-160.
Bazarghani, T.T., Charkhkar, S., Doroudi, J. and Bani Hassan, E. (2006). A Review on Peste des Petits Ruminants (PPR) with Special Reference to PPR in Iran. Journal of Veterinary Medicine Series B, 53(s1): 17-18.
Cam, Y., Gencay, A., Beyaz, L., Atalay, O., Atasever, A., Özkul, A., et al. (2005). Peste des petits ruminants in a sheep and goat flock in Kayseri province, Turkey. Veterinary Record, 157: 523-524.
Chauhan, H.C., Chandel, B.S., Kher, H.N., Dadawala, A.I. and Agrawal, S.M. (2009). Pesti des petits ruminants virus infection in animals. Veterinary World, 2(4): 443-447.
Davari, S.A., Sayyari, M. and Mohammadi, A. (2013). Genetic analysis of the viral agents causing muzzle crust in small ruminants of Shiraz, Iran. Bulgarian Journal of Veterinary Medicine, 16(3): 159-169.
Diallo, A., Minet, C., Le Goff, C., Berhe, G., Albina, E., Libeau, G., et al. (2007). The threat of peste des petits ruminants: progress in vaccine development for disease control. Vaccine, 25(30): 5591-5597.
Esmaelizad, M., Jelokhani, S. and Kargar-Moakhar, R. (2011). Phylogenetic analysis of peste des petits ruminants virus (PPRV) isolated in Iran based on partial sequence data from the fusion (F) protein gene. Turkish Journal of Biology, 35(1): 45-50.
Fallahi, R. (2017). Isolation and molecular diagnosis of Peste des petits ruminants (PPR) virus from contaminated areas in Iran. Iranian Journal of Veterinary Science and Technology, 9(1): 17-21.
Foroughi, A., Chaharaein, B., Ownagh, A. and Mardani, K. (2013). Comparison of c-ELISA and RT-PCR methods in detection of Peste Des Petits Ruminants in early stage from clinical specimens in goats and sheep in Kermanshah Province. Experimental Animal Biology, 2(1): 51-56. [In Persian]
Kataria, A.K., Kataria, N. and Gahlot, A.K. (2007). Large scale outbreaks of peste des petits ruminants in sheep and goats in Thar Desert of India. Slovenian Veterinary Research, 44(4): 123-132.
Khan, H.A., Siddique, M., Arshad, M.J., Khan, Q.M. and Rehman, S.U. (2007). Sero-prevalence of peste des petits ruminants (PPR) virus in sheep and goats in Punjab province of Pakistan. Pakistan Veterinary Journal, 27(3): 109-112.
Khan, M.A., Hussain, S.N., Bahadar, S., Ali A. and Shah I.A. (2008). An outbreak of peste des petits ruminants (PPR) in goats in district Chitral, N.W.F.P., Pakistan. ARPN Journal of Agricultural and Biological Science, 3(2): 19-22.
Khan, M.R., Haider, M.G., Alam, K.J., Hossain, M.G., Chowdhury, S.M.Z.H. and Hossain, M.M. (2012). Pathological investigation of Peste des Petits Ruminants (PPR) in goats. Bangladesh Journal of Veterinary Medicine, 3(2): 134-138.
Mahajan, V., Filia, G., Bal, M.S., Kaur, G., Sharma, S., Dantotia, A., et al. (2017). Outbreaks of Peste Des Petits Ruminants (PPR) in goats in Punjab, India. International Journal of Current Microbiology and Applied Sciences, 6(8): 3705-3710.
Marashi, M., Masoudi, S., Moghadam, M.K., Modirrousta, H., Marashi, M., Parvizifar, M., et al. (2017). Peste des Petits Ruminants virus in vulnerable wild small ruminants, Iran, 2014-2016. Emerging Infectious Diseases, 23(4): 704-706.
Mokhtari, A., Azizi, Z. and Rabiaee Fradonbeh, S. (2017). Epidemiological study and spatial modeling of peste des petits ruminants (PPR) in central area of Iran. Revista MVZ Córdoba, 22(2): 5899-5909.
Özkul, A., Akca, Y., Alkan, F., Barrett, T., Karaoglu, T., Dagalp, S.B. and Burgu, I. (2002). Prevalence, distribution, and host range of Peste des petits ruminants virus, Turkey. Emerging Infectious Diseases, 8(7): 709-712.
Patel, J.M., Patel, D.R., Mavadiya, S.V., Solanki, J.B., Vihol, P.D., Sharma, K.K., et al. (2015). Clinicopathological investigation of an outbreak of peste des petits ruminants in small ruminants in South Gujarat, India. Indian Journal of Veterinary Pathology, 39(1): 20-23.
Rajak, K.K., Sreenivasa, B.P., Hosamani, M., Singh, R.P., Singh, S.K., Singh, R.K., et al. (2005). Experimental studies on immunosuppressive effects of peste des petits ruminants (PPR) virus in goats. Comparative Immunology, Microbiology and Infectious Diseases, 28(4): 287-296.
Rezazadeh, F., Madadgar, O. and Poureini, F. (2016). Study of Peste Des Petits Ruminants (PPR) in some border Areas of Iran by Nested- PCR. Iranian Journal of Ruminants Health Research, 1(1): 61-72.
Singh, R.P., Saravanan, P., Sreenivasa, B.P., Singh, R.K. and Bandyopadhyay, S.K. (2004). Prevalence and distribution of peste des petits ruminant’s virus infection in small ruminant’s in India. Revue Scientifique Et Technique, 23(3): 807-819.
Toplu, N. (2004). Characteristic and non-characteristic pathological findings in peste des petits ruminant’s (PPR) of sheep in the Ege district of Turkey. Journal of Comparative Pathology, 131(2): 135-141.
Truong, T., Boshra, H., Embury-Hyatt, C., Nfon, C., Gerdts, V., Tikoo, S., et al. (2014). Peste des petits ruminants virus tissue tropism and pathogenesis in sheep and goats following experimental infection. PLOS ONE, 9(1): e87145.
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Abdollahpour, G., Raoofi, A., Najafi, J., Sasani, F. and Sakhaie, E. (2006). Clinical and paraclinical findings of a recent outbreaks of Peste des petits ruminants in Iran. Zoonoses and Public Health, 53(s1): 14-16.
Abubakar, M., Ali, Q. and Khan, H.A. (2008). Prevalence and mortality rate of peste des petitis ruminant (PPR): possible association with abortion in goat. Tropical Animal Health and Production, 40(5): 317-321.
Al-Dubaib, M.A. (2009). Peste des petitis ruminants morbillivirus infection in lambs and young goats at Qassim region, Saudi Arabia. Tropical Animal Health and Production, 41(2): 217-220.
Al-Naeem, A., Abu, E.E. and Al-Afaleq, A.I. (2000). Epizootiological aspects of peste des petits ruminants and rinderpest in sheep and goats in Saudi Arabia. Revue Scientifique Et Technique, 19(3): 855-858.
Aruni, A.W., Lalitha, P.S., Mohan, A.C., Chitravelu, P. and Anbumani, S.P. (1998). Histopathological study of a natural outbreak of peste des petits ruminants in goats of Tamilnadu. Small Ruminant Research, 28(3): 233-240.
Atta-ur-Rahman, M.A., Rahman, S.U., Akhtar, M. and Ullah, S. (2004). Peste des petits ruminants antigen in mesenteric lymph nodes of goats slaughtered at DI Khan. Pakistan Veterinary Journal, 24(3): 159-160.
Bazarghani, T.T., Charkhkar, S., Doroudi, J. and Bani Hassan, E. (2006). A Review on Peste des Petits Ruminants (PPR) with Special Reference to PPR in Iran. Journal of Veterinary Medicine Series B, 53(s1): 17-18.
Cam, Y., Gencay, A., Beyaz, L., Atalay, O., Atasever, A., Özkul, A., et al. (2005). Peste des petits ruminants in a sheep and goat flock in Kayseri province, Turkey. Veterinary Record, 157: 523-524.
Chauhan, H.C., Chandel, B.S., Kher, H.N., Dadawala, A.I. and Agrawal, S.M. (2009). Pesti des petits ruminants virus infection in animals. Veterinary World, 2(4): 443-447.
Davari, S.A., Sayyari, M. and Mohammadi, A. (2013). Genetic analysis of the viral agents causing muzzle crust in small ruminants of Shiraz, Iran. Bulgarian Journal of Veterinary Medicine, 16(3): 159-169.
Diallo, A., Minet, C., Le Goff, C., Berhe, G., Albina, E., Libeau, G., et al. (2007). The threat of peste des petits ruminants: progress in vaccine development for disease control. Vaccine, 25(30): 5591-5597.
Esmaelizad, M., Jelokhani, S. and Kargar-Moakhar, R. (2011). Phylogenetic analysis of peste des petits ruminants virus (PPRV) isolated in Iran based on partial sequence data from the fusion (F) protein gene. Turkish Journal of Biology, 35(1): 45-50.
Fallahi, R. (2017). Isolation and molecular diagnosis of Peste des petits ruminants (PPR) virus from contaminated areas in Iran. Iranian Journal of Veterinary Science and Technology, 9(1): 17-21.
Foroughi, A., Chaharaein, B., Ownagh, A. and Mardani, K. (2013). Comparison of c-ELISA and RT-PCR methods in detection of Peste Des Petits Ruminants in early stage from clinical specimens in goats and sheep in Kermanshah Province. Experimental Animal Biology, 2(1): 51-56. [In Persian]
Kataria, A.K., Kataria, N. and Gahlot, A.K. (2007). Large scale outbreaks of peste des petits ruminants in sheep and goats in Thar Desert of India. Slovenian Veterinary Research, 44(4): 123-132.
Khan, H.A., Siddique, M., Arshad, M.J., Khan, Q.M. and Rehman, S.U. (2007). Sero-prevalence of peste des petits ruminants (PPR) virus in sheep and goats in Punjab province of Pakistan. Pakistan Veterinary Journal, 27(3): 109-112.
Khan, M.A., Hussain, S.N., Bahadar, S., Ali A. and Shah I.A. (2008). An outbreak of peste des petits ruminants (PPR) in goats in district Chitral, N.W.F.P., Pakistan. ARPN Journal of Agricultural and Biological Science, 3(2): 19-22.
Khan, M.R., Haider, M.G., Alam, K.J., Hossain, M.G., Chowdhury, S.M.Z.H. and Hossain, M.M. (2012). Pathological investigation of Peste des Petits Ruminants (PPR) in goats. Bangladesh Journal of Veterinary Medicine, 3(2): 134-138.
Mahajan, V., Filia, G., Bal, M.S., Kaur, G., Sharma, S., Dantotia, A., et al. (2017). Outbreaks of Peste Des Petits Ruminants (PPR) in goats in Punjab, India. International Journal of Current Microbiology and Applied Sciences, 6(8): 3705-3710.
Marashi, M., Masoudi, S., Moghadam, M.K., Modirrousta, H., Marashi, M., Parvizifar, M., et al. (2017). Peste des Petits Ruminants virus in vulnerable wild small ruminants, Iran, 2014-2016. Emerging Infectious Diseases, 23(4): 704-706.
Mokhtari, A., Azizi, Z. and Rabiaee Fradonbeh, S. (2017). Epidemiological study and spatial modeling of peste des petits ruminants (PPR) in central area of Iran. Revista MVZ Córdoba, 22(2): 5899-5909.
Özkul, A., Akca, Y., Alkan, F., Barrett, T., Karaoglu, T., Dagalp, S.B. and Burgu, I. (2002). Prevalence, distribution, and host range of Peste des petits ruminants virus, Turkey. Emerging Infectious Diseases, 8(7): 709-712.
Patel, J.M., Patel, D.R., Mavadiya, S.V., Solanki, J.B., Vihol, P.D., Sharma, K.K., et al. (2015). Clinicopathological investigation of an outbreak of peste des petits ruminants in small ruminants in South Gujarat, India. Indian Journal of Veterinary Pathology, 39(1): 20-23.
Rajak, K.K., Sreenivasa, B.P., Hosamani, M., Singh, R.P., Singh, S.K., Singh, R.K., et al. (2005). Experimental studies on immunosuppressive effects of peste des petits ruminants (PPR) virus in goats. Comparative Immunology, Microbiology and Infectious Diseases, 28(4): 287-296.
Rezazadeh, F., Madadgar, O. and Poureini, F. (2016). Study of Peste Des Petits Ruminants (PPR) in some border Areas of Iran by Nested- PCR. Iranian Journal of Ruminants Health Research, 1(1): 61-72.
Singh, R.P., Saravanan, P., Sreenivasa, B.P., Singh, R.K. and Bandyopadhyay, S.K. (2004). Prevalence and distribution of peste des petits ruminant’s virus infection in small ruminant’s in India. Revue Scientifique Et Technique, 23(3): 807-819.
Toplu, N. (2004). Characteristic and non-characteristic pathological findings in peste des petits ruminant’s (PPR) of sheep in the Ege district of Turkey. Journal of Comparative Pathology, 131(2): 135-141.
Truong, T., Boshra, H., Embury-Hyatt, C., Nfon, C., Gerdts, V., Tikoo, S., et al. (2014). Peste des petits ruminants virus tissue tropism and pathogenesis in sheep and goats following experimental infection. PLOS ONE, 9(1): e87145.
